Reference no: EM133935296

Question

Henrietta Lacks was an African American who died in 1951 of a cancerous tumor in very rapid development. During his processing, samples were taken without his consent, and were subsequently cultured in the laboratory. These cells are ultimately became the immortal HeLa cell line, a cell line that revolutionized research biomedical, and led to many pharmaceutical discoveries. The contribution of Henrietta in contemporary research has recently been recognized, and can be considered as the first immortal / zombie woman, the first to be in space, and the most important for biomedical studies.

Although immortal, chemical compounds toxic to human cells can kill HeLa cells. Your laboratory work led to the discovery of a non-chemical compound toxic produced by the intestine of the worm Caenorhabditis elegans. However, you also have discovered that HeLa cells can metabolize compound into a toxic product. In the in order to save the HeLa cell line, your supervisor asks you to identify the genes encoding the proteins involved in this metabolic pathway. In addition, this must be done screening with a large-scale method in order to save HeLa cells from extinction. Suggest an approach to identify all (or most) of these genes. Describe the methods, what you are going to measure and the expected results.