Calculating total magnification for each objective.

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Be familiar with lab safety regulations and practices for microbiology lab

Be able to properly label parts of the microscope.

Know how to correctly handle and focus the microscope.

What is the purpose of immersion oil?

How to use oil immersion lens

Calculating total magnification for each objective

What light adjustment did you need to perform when going from 40x to 100x oil immersion?

What is the purpose of course adjustment? And fine adjustment?

Be able to ID from micrographs obvious bacterial shapes and arrangements

Know and understand the importance of aseptic technique: hand washing with antibacterial soap before and after the lab, disinfection of tables before and after the lab, and using the proper procedures for handling microbes, etc....

Know how to properly handle and use the inoculation loop: How is an inoculating loop sterilized? Why is it important to avoid damaging the agar with the loop? Why is it important to flame metal inoculating loop prior to and after each inoculation? Why is it important to cool the inoculating tool before obtaining the inoculum?

Know how to properly use the bacticinerator

What is a culture in microbiology? What is a pure culture? How can you tell if your culture is contaminated?

What is the purpose of streaking bacteria for isolation?

Demo a streak plate for isolation of pure colonies if asked. What are the steps? Which part of the plate should have most growth? Which part will have isolated colonies?

If you didn't obtain isolated colonies on your streak plate, suggest why this may have happened?

How can you determine if your plate has been contaminated? What factors could've caused contamination? Is there anything you could you have done to avoid contamination?

Why do we handle and place the inoculated agar plates in an inverted position in the incubator?

When using tube cultures, why is it important to flame the neck of the tubes after uncapping and before replacing the cap? Why is it essential to hold test tube caps in your hand?

Know how to do a regular wet mount and how it is different from hanging drop mount.

What is a purpose of a hanging drop preparation? Are bacteria stained for hanging drop?

Explain the difference between true motility and Brownian motion

Which method is better at detecting motility: hanging drop of motility agar?

How does motility agar work? How can you tell if your organism is positive for motility or negative? Know how to interpret results. Is it possible to get false positive motility results?

Why is TTC added to motility agar? Why do you need a negative control?

Reference no: EM133893540

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