Reference no: EM133261159
1. Why are bonds between amino acids "flat"?
ALL OF THESE
NONE OF THESE
Because of their partial double-bond character
All carbon-nitrogen bonds are planar.
Long-distance bonding between the nitrogen and oxygen limits movement.
2. Gradient centrifugation is commonly used for which of the following?
isolating small molecules
measuring the concentration of proteins in a solution
separating organelles out of a homogenate
separating proteins in a solution
3. 2-D electrophoresis involves
affinity chromatography and SDS-PAGE
isoelectric focusing and affinity chromatography
isoelectric focusing and ion-exchange chromatography
ion-exchange chromatography and SDS-PAGE
isoelectric focusing and SDS-PAGE
4. Some amino acids are called "nonessential" because
your body can make them
they are not necessary for your health
you can eat them
you have so many of them
they are not used to make proteins
5. Alpha helices, beta sheets, and turns are examples of......structure.
secondary
tertiary
primary
quaternary
6. Which of the following would have the most entropy?
molten globule
unfolded polypeptide
amyloid fibril
beta sheet
alpha helix
7. Which of the following can change the proteins found in a cell?
environmental conditions
NONE OF THESE
ALL OF THESE
enzymatic modifications
developmental stage
8. Which of the following can serve as antigens?
proteins and polysaccharides
proteins and small molecules
polysaccharides and ions
ALL OF THESE
ions and small molecules
NONE OF THESE
9. Essential amino acids are....
hydrophilic
the 20 that proteins are made of
polar
hydrophobic
important parts of your diet
10. Which of the following is NOT true of immunoprecipitation?
A) separates particles using an electric field
B) uses antibody-coated beads
C) good for isolating specific proteins from a solution
D) includes a denaturing step
11. Protein size is often determined using...
A) isoelectric-focusing gel
B) Edman degradation
C) SDS polyacrylamide gel electrophoresis
D) affinity chromatography
E) ELISA
12. Which of the following states of a protein would be the most stable?
A) amyloid fibril state
B) molten globule state
C) unfolded state
D) intermediate state
E) native state
13. Which pair of amino acids are negatively charged at pH 7?
A) glutamine and histidine
B) aspartic acid and glutamate
C) lysine and arginine
D) alanine and glutamate
E) lysine and proline
14-Which of the following qualities is most frequently used to measure the amount of a substance in a solution?
A) shape
B) density
C) size
D) weight
D) light absorbance
15. According to convention, the terminus is drawn on the left side of a peptide.
5'
carboxy
amino
3'
16. Which of the following is the usual order in the process of protein purification?
centrifugation, homogenation, chromatography, electrophoresis
homogenation, chromatography, electrophoresis, centrifugation
homogenation, electrophoresis, centrifugation, chromatography
homogenation, centrifugation, chromatography, electrophoresis
17. Which of the following is characterized by "multiple subunits"?
secondary structure
tertiary structure
quaternary structure
protein
primary structure
18. Misfolded proteins are not responsible for....
Alzheimer Disease
Parkinson Disease
Huntington Disease
kwashiorkor
prion disease
19. The protein structure where a series of amino acids are each bonded with the fourth amino acid down the chain is the....
alpha helix
beta sheet
coiled coil
turn
20. Which of the following amino acids would be LEAST likely to be found on the surface of a globular protein?
lysine
glutamine
serine isoleucine
cysteine