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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Which type Ribosome occurs exclusivley in Mitocondria?
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Planning the Nursing Care Monitor fluid intake and urinary output Administer drugs as advised/prescribed Monitor the child on dialysis Provide therapeutic diet
Explain The absorption of itraconazole capsules It is decreased with concurrent use of drugs that reduce gastric acidity, such as antacids, H2-receptor blockers, proton pump in
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Suppose that the type of drink did not affect which can floated or sank. May be the cans themselves were different in some way. May be something besides soda got into one of the ca
Explain Relative Quantitative difference of Protein Taxonomy This analysis involves the study between the amounts of different proteins and more significantly, of different con
Tertiary Butylhydroquinone (TBHQ) TBHQ is moderately soluble in oil and slightly soluble in water. In many cases, TBHQ is more effective than any other antioxidant in providing
Define Iron Factors affecting absorption of dietary iron? We have read earlier that iron in foods occurs either as haem or non-haem iron. Haem iron comprises of iron in combina
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