Retroviral method for gene transfer, Biology

Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication.  The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.

Posted Date: 9/18/2012 7:49:49 AM | Location : United States







Related Discussions:- Retroviral method for gene transfer, Assignment Help, Ask Question on Retroviral method for gene transfer, Get Answer, Expert's Help, Retroviral method for gene transfer Discussions

Write discussion on Retroviral method for gene transfer
Your posts are moderated
Related Questions
Q. Implementation of the Plan in diabetes mellitus? You have helped the client to find out an alternative solution and to develop a plan to solve the problem. Now, it has to be

The major ecological imbalance on Earth is between the human species and the rest of Earth's biota. What are two specific actions we could do to achieve a balance (for example, rec

Q. What is the anatomical reason for the left bronchus to be more elevated than the right bronchus? Why in most cases of aspiration of foreign material by children is the object fo

Q. What are twins? Genetically what are the two types of twins that can be generated? Twins are at the same time generated (within the mother's uterus) offspring. Twins classif

Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-

Dose-response extrapolation In order to be compared to human exposure levels, the animal data need to be extrapolated to doses much lower than those studied. This extrapolation


Valve Thrombosis :  Valve thrombosis causes sudden deterioration of the patient's haemodyarnics. A stuck valve may produce both stenosis and incompetence. If diagnosed early s

Describe the term Epinephrine? Epinephrine is administered IV in increments of 1 mg diluted in 10 mls of normal saline (1:10,000). It can be given through the endotracheal tube

Growing leaves in the classroom A sweet potato will make dense foliage in the classroom if it is placed in water. Set the potato, root end down, in a glass or jar and keep the