Reagent for determination of blood glucose by nelson somogyi, Biology

Define Reagent for Determination of Blood Glucose by Nelson Somogyi?

1. Alkaline copper reagent

a) Dissolve 15 g of sodium potassium tartarate and 30 g of anhydrous Na2 CO3 in about 300 ml of water. Add 20 g NaHCO3 . Dissolve 180 g of anhydrous Na2 SO4 in 500 ml of boiling water and cool. Mix the two solutions and make up to 1Litre with water.

b) Dissolve 5 g CuSO4 .5H2 O and 45 g anhydrous Na2 SO4 in H2 O and make upto 250 ml. c)Mix reagents A (4 vol) and B (1 vol) just before use.

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2 SO4 with stirring. Dissolve 3 g Na2 HAsO4 .7H2 O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

3. Sodium sulphate copper-copper sulphate solution-13.2 g of anhydrous sodium sulphate and 6 g of copper sulphate in 1 litre of water

4. Stock standard - 1mg glucose/ml of isotonic sodium sulphate- copper sulphate solution.

5. Working standard solution-Dilute the stock standard (1 ml and 2.5 ml) to 100 ml with isotonic solution.

Posted Date: 6/17/2013 5:56:01 AM | Location : United States

Related Discussions:- Reagent for determination of blood glucose by nelson somogyi, Assignment Help, Ask Question on Reagent for determination of blood glucose by nelson somogyi, Get Answer, Expert's Help, Reagent for determination of blood glucose by nelson somogyi Discussions

Write discussion on Reagent for determination of blood glucose by nelson somogyi
Your posts are moderated
Related Questions
Interactions between Volatile Substances and Proteins               Flavour binding may involve adsorption at the surface of food or penetration to the food interiority by diffu

Implantation - Pre-Embryonic Development After entering the uterus and formation of ICM, the blastocyst starts to embed in the endometrium of the uterine wall. By one week aft

Energy Metabolism In the preceding sections of the unit, you studied how the products of digestion of food, viz: amino acids, sugars and fatty acids are absorbed and transport

Explain saturated fatty acids Some of the more significant saturated fatty acids with their systematic and common names are listed in table, and some of the unsaturated fatty a

What are the valves of the venous system? What is their function? The valves of the venous system are structures within the veins that permit blood to flow only in the normal w

Explain the Basic Concept of Proteins? Proteins, as we already knows by now are products of amino acids. Each molecule of protein is composed of many molecules of amino acids j

State the term - Symbolic representation Symbolic representation is the representation of both the external and internal world through symbols and has been discussed in relatio

Explain Procedure for Checking the Presence of Rhodamine B? Conduct the experiment following the procedure given herewith: 1. Pour liquid paraffin or mineral oil in a Petri

Scope of zoology

according to the graph,which age is the amount og growth hormone produced at its highest?