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Define Reagent for Determination of Blood Glucose by Nelson Somogyi?
1. Alkaline copper reagent
a) Dissolve 15 g of sodium potassium tartarate and 30 g of anhydrous Na2 CO3 in about 300 ml of water. Add 20 g NaHCO3 . Dissolve 180 g of anhydrous Na2 SO4 in 500 ml of boiling water and cool. Mix the two solutions and make up to 1Litre with water.
b) Dissolve 5 g CuSO4 .5H2 O and 45 g anhydrous Na2 SO4 in H2 O and make upto 250 ml. c)Mix reagents A (4 vol) and B (1 vol) just before use.
2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2 SO4 with stirring. Dissolve 3 g Na2 HAsO4 .7H2 O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.
3. Sodium sulphate copper-copper sulphate solution-13.2 g of anhydrous sodium sulphate and 6 g of copper sulphate in 1 litre of water
4. Stock standard - 1mg glucose/ml of isotonic sodium sulphate- copper sulphate solution.
5. Working standard solution-Dilute the stock standard (1 ml and 2.5 ml) to 100 ml with isotonic solution.
Define Metabolic Aberrations and Clinical Manifestations? The state of obesity brings about certain alterations in the normal body processes which are enumerated herewith and h
keel is the characteristic feature of flower of 1.tomato 2. tulip 3.ubdugifera 4.aloe
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