Reagent for determination of blood glucose by nelson somogyi, Biology

Define Reagent for Determination of Blood Glucose by Nelson Somogyi?

1. Alkaline copper reagent

a) Dissolve 15 g of sodium potassium tartarate and 30 g of anhydrous Na2 CO3 in about 300 ml of water. Add 20 g NaHCO3 . Dissolve 180 g of anhydrous Na2 SO4 in 500 ml of boiling water and cool. Mix the two solutions and make up to 1Litre with water.

b) Dissolve 5 g CuSO4 .5H2 O and 45 g anhydrous Na2 SO4 in H2 O and make upto 250 ml. c)Mix reagents A (4 vol) and B (1 vol) just before use.

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2 SO4 with stirring. Dissolve 3 g Na2 HAsO4 .7H2 O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

3. Sodium sulphate copper-copper sulphate solution-13.2 g of anhydrous sodium sulphate and 6 g of copper sulphate in 1 litre of water

4. Stock standard - 1mg glucose/ml of isotonic sodium sulphate- copper sulphate solution.

5. Working standard solution-Dilute the stock standard (1 ml and 2.5 ml) to 100 ml with isotonic solution.

Posted Date: 6/17/2013 5:56:01 AM | Location : United States







Related Discussions:- Reagent for determination of blood glucose by nelson somogyi, Assignment Help, Ask Question on Reagent for determination of blood glucose by nelson somogyi, Get Answer, Expert's Help, Reagent for determination of blood glucose by nelson somogyi Discussions

Write discussion on Reagent for determination of blood glucose by nelson somogyi
Your posts are moderated
Related Questions
Q. Physical Signs of mitral regurgitation? Pulse is of normal character but carotid upstroke may be brisk. Atrial fibrillation is often present in a patient with advanced disea

Q Show Rules followed by diabetic patients? A  "sick day" is any health problem diabetic patient may have. This could include a common cold, infection, diarrhoea, fever, injury

STRUCTURE OF WATER It may interest you to know that water is a universal solvent and is a major constituent of all living organisms. Earth is the only planet where water exists i

what is the bet way to asses energy production in the body of an athlete?

Plant sources Although there is a multitude of colours in the plant kingdom, their extraction and use in food systems is not an easy task. Unless the colourants have some outst

NEUROPSYCHOLOGICAL BATTERY The battery was published in 1980 by Western Psychological Services and is now extensively used in clinical and research applications. An alternate

Types of Development of Animals Different animals have evolved various methods of development. These methods can be broadly categorized into two categories (i) Direct devel

Define Criteria for Assessment of Vitamin B 12 Status? Low serum or plasma levels of vitamin B 12 should be the first indication of poor  status and this could be confirmed

SODIUM The element is available in table salt. A proper balance of sodium and potassium is essential. Its absorption is under control of aldasterone. The various f

Remove the crown for reuse - If the decision is made to remove the crown for reuse - The visibility is increased - Allowing for much easier removal of canal obstructions -