Reagent for determination of blood glucose by nelson somogyi, Biology

Assignment Help:

Define Reagent for Determination of Blood Glucose by Nelson Somogyi?

1. Alkaline copper reagent

a) Dissolve 15 g of sodium potassium tartarate and 30 g of anhydrous Na2 CO3 in about 300 ml of water. Add 20 g NaHCO3 . Dissolve 180 g of anhydrous Na2 SO4 in 500 ml of boiling water and cool. Mix the two solutions and make up to 1Litre with water.

b) Dissolve 5 g CuSO4 .5H2 O and 45 g anhydrous Na2 SO4 in H2 O and make upto 250 ml. c)Mix reagents A (4 vol) and B (1 vol) just before use.

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2 SO4 with stirring. Dissolve 3 g Na2 HAsO4 .7H2 O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

3. Sodium sulphate copper-copper sulphate solution-13.2 g of anhydrous sodium sulphate and 6 g of copper sulphate in 1 litre of water

4. Stock standard - 1mg glucose/ml of isotonic sodium sulphate- copper sulphate solution.

5. Working standard solution-Dilute the stock standard (1 ml and 2.5 ml) to 100 ml with isotonic solution.


Related Discussions:- Reagent for determination of blood glucose by nelson somogyi

Maintenance of the normal quantity of chromosomes, Q. Why is meiosis signif...

Q. Why is meiosis significant for the maintenance of the normal quantity of chromosomes of a species with sexual reproduction? A reduction to a half of the maximum normal quant

How are gases exchanged in sponges, Q. How are gases exchanged in sponges? ...

Q. How are gases exchanged in sponges? The gas exchange in sponges happens by diffusion from the exterior to the cells that absorb molecular oxygen and liberate carbon dioxide.

Kin and group selection, Before we proceed to discuss kin selection we shou...

Before we proceed to discuss kin selection we should define the term altruism. It refers to the behaviour pattern of an individual in the population for the benefit of other member

Acupunture-osteopathy-herbal therapy, Alternative Therapies Assignment 1...

Alternative Therapies Assignment 1. A. Acupunture, B.  Osteopathy, C.  Herbal therapy For each one of alternative therapies above, answers the three questions below! 1. Th

Explain the natural history of coronary artery diseases, Explain the NATURA...

Explain the NATURAL HISTORY OF CORONARY ARTERY DISEASES (GAD)? The natural history of CAD is very important from the preventive point or view. Though the usual manifestations o

Arterial blood gas studies, Arterial Blood Gas Studies: Purpose 1)  A me...

Arterial Blood Gas Studies: Purpose 1)  A measurement of partial pressure of oxygen and carbon dioxide  in arterial blood, as well as the pH  of  the blood.  2) The Partial p

Hypoglycemic effects of dietary factor, Define Hypoglycemic effects of Diet...

Define Hypoglycemic effects of Dietary Factor with antinutritional effect? Of the different carbohydrate foods tested for starch digestibility and blood glucose response, those

Porifera, method of digestion in porifera

method of digestion in porifera

What are the values of dpd for plant cells under hypertonic, What are the v...

What are the values of DPD for plant cells under hypertonic, isotonic and hypotonic media? In plant cells under hypertonic medium there is loss of water for the exterior, SF >

Medical records, Medical Records This includes: health history o...

Medical Records This includes: health history of patient/client  a report of the findings on examination  signs and symptoms  observation records  progress

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd