Reagent for determination of blood glucose by nelson somogyi, Biology

Define Reagent for Determination of Blood Glucose by Nelson Somogyi?

1. Alkaline copper reagent

a) Dissolve 15 g of sodium potassium tartarate and 30 g of anhydrous Na2 CO3 in about 300 ml of water. Add 20 g NaHCO3 . Dissolve 180 g of anhydrous Na2 SO4 in 500 ml of boiling water and cool. Mix the two solutions and make up to 1Litre with water.

b) Dissolve 5 g CuSO4 .5H2 O and 45 g anhydrous Na2 SO4 in H2 O and make upto 250 ml. c)Mix reagents A (4 vol) and B (1 vol) just before use.

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2 SO4 with stirring. Dissolve 3 g Na2 HAsO4 .7H2 O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

3. Sodium sulphate copper-copper sulphate solution-13.2 g of anhydrous sodium sulphate and 6 g of copper sulphate in 1 litre of water

4. Stock standard - 1mg glucose/ml of isotonic sodium sulphate- copper sulphate solution.

5. Working standard solution-Dilute the stock standard (1 ml and 2.5 ml) to 100 ml with isotonic solution.

Posted Date: 6/17/2013 5:56:01 AM | Location : United States







Related Discussions:- Reagent for determination of blood glucose by nelson somogyi, Assignment Help, Ask Question on Reagent for determination of blood glucose by nelson somogyi, Get Answer, Expert's Help, Reagent for determination of blood glucose by nelson somogyi Discussions

Write discussion on Reagent for determination of blood glucose by nelson somogyi
Your posts are moderated
Related Questions
How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?

Define Interaction of Vitamin K with Vitamins A and E? Excess vitamin A interferes with vitamin K absorption while the a-tocopherol or vitamin E, as we have already studied ear

Explain the types of foods used in Space? The various types of foods are enumerated herewith. Thennostabilized (T): Heat processed foods ("off-the-shelf' items) in aluminium

Define the density of egg  The density of egg products is not affected by dehydration. When a dried egg product is reconstituted to its natural solids, it has about the same d

A study is made to verify the effects of pesticide exposure on pupal weight of butterflies. In a pilot study, 5 randomly selected larvae are raised on plants exposed to the pestici

Define about the Cellulose acetate electrophoresis (CAE)? If the hydroxyl groups in cellulose are reacted with acetic anhydride, cellulose is acetylated to form the raw materia

What is sustainability? Sustainability is the ability to meet human needsin a way that will allow a human population to survive indefinitely.

Q. Do protozoans have asexual or sexual reproduction ? In protozoans reproduction is asexual or sexual. The most frequent form of sexual reproduction is binary scissiparity or d

B o v i ne rotavirus diarrhoea The bovine rotavirus is a RNA virus with 11 segments of double stranded RNA belonging to the genus Rotavirus in the family Reoviridae. Rotavi

What is the cell division process directly related to the embryonic growth? The embryonic growth depends directly on mitosis. By this type of cell division the zygote separates