Preparation of dilutions - standard or total plate counts, Biology

Assignment Help:

Define Preparation of Dilutions - Standard or Total Plate Counts?

1. Serial dilution of homogenized sample in an appropriate diluent.

Preparation of dilutions - Decimal dilutions are prepared in a diluent [peptone water (1%) and 0.5% NaCl] and some dilutions are plated. Selection of the dilutions to be plated is made on the basis of expected microbial load in the sample.

2. Plating on to suitable agar medium by pour plate or surface plating.

3. Incubation of plates at appropriate temperature (usually 35-37°C) for a given time (24-48 hours).

4. Counting of all visible colonies manually or using a Quebec or electronic counter.

5. Calculating CFU/ml or CFU/gm of the food sample.


Related Discussions:- Preparation of dilutions - standard or total plate counts

What are the dominate phenotype, There is a population of 100 frogs. 20 are...

There is a population of 100 frogs. 20 are heterozygotes, 30 are homozygous recessive, 25 are homozygous dominate. What is the frequency of the dominate allele? The recessive allel

Explain functional properties of gellan gums, Explain functional properties...

Explain functional properties of gellan gums One of the most important features of gellan gums is its versatile texture which is defined in terms of hardness (measure of ruptur

How hyperventilation affect blood ph, Freediving is an activity in which a ...

Freediving is an activity in which a person dives, sometimes to great depth, without the use of scuba gear. The diver must hold his or her breath for the duration of the dive. (The

Explain procambium in primary growing shoot, Explain Procambium in primary ...

Explain Procambium in primary growth in shoot ? The vascular tissue in the shoot is produced from a primary tissue called the procambium, located in the apical meristem. New vas

Explain functions of proteins, Explain Functions of proteins Functions ...

Explain Functions of proteins Functions of proteins are included herewith. These include: Source of energy: Constituent amino acids can be deaminated and metabolized to

What is the destination of the branchial clefts in humans, What is the dest...

What is the destination of the branchial clefts in humans? In humans the branchial clefts situated in the anterior region of the pharynx (also called as pharyngeal clefts) are

Explain the equilibrium dialysis, Explain the Equilibrium Dialysis? Equ...

Explain the Equilibrium Dialysis? Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The mac

Excretory system, what is the excretory organ in protozoa?

what is the excretory organ in protozoa?

Impact of technological change on the cost of health service, Impact of Tec...

Impact of Technological Change on the Cost of Health Service Technological developments entail improvement in production/service frontiers either by providing cost benefit adv

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd