Explain the equilibrium dialysis, Biology

Assignment Help:

Explain the Equilibrium Dialysis?

Equilibrium dialysis is a useful technique for studying the binding of a small uncharged solute species (a ligand) to a macromolecule. The macromolecule solution is placed on one side of a membrane through which it cannot pass, with a solution without the macro- molecule on the other side, and the ligand is allowed to come to transfer equilibrium across the membrane. If the same solute standard state is used for the ligand in both solutions, at equilibrium the unbound ligand must have the same activity in both solutions. Measure- ments of the total ligand molality in the macromolecule solution and the ligand molality in the other solution, combined with estimated values of the unbound ligand activity coefficients, allow the amount of ligand bound per macromolecule to be calculated.


Related Discussions:- Explain the equilibrium dialysis

St-segment depression after hyperventilation, Q. ST-segment depression aft...

Q. ST-segment depression after  hyperventilation? Patients with abnormal autonomic drive have demonstrated ST-segment depression after hyperventilation as well as after exercis

Which statement about asexual reproduction is false, Which statement below ...

Which statement below about asexual reproduction is FALSE? a.With asexual reproduction, offspring are genetically identical to the parent. b.Asexual reproduction requires no

What is atopic dermatitis, Atopic dermatitis is an inflammatory skin diseas...

Atopic dermatitis is an inflammatory skin disease. This disease is observed frequently in young children. There will be skin eruptions.

What are the major characteristics of the bryophytes, Q. What are the major...

Q. What are the major characteristics of the bryophytes? Bryophytes are nonvascular plants that is they do not have conductive tissues and they perform transport of nutrients a

Explain the lactose fermenters and non lactose fermenters, Explain the Lact...

Explain the Lactose Fermenters and Non lactose Fermenters 1) Lactose Fermenters - These produce acid on lactose fermentation, which results in red colouration on bacterial colo

Conduction system - heart, In this unit, you have learnt that heart is a mu...

In this unit, you have learnt that heart is a muscular organ situated in thorax covering with pericardium and consist of four chambers i.e. right atrium, left atrium, right ventric

Discuss in detail about the mitochondria, Discuss in detail about the Mitoc...

Discuss in detail about the Mitochondria An important function of the cell is to derive energy from the different oxidisable nutrients, such as, carbohydrates and fats supplied

What are the phytoplankton and the zooplankton, What are the phytoplankton ...

What are the phytoplankton and the zooplankton? Phytoplankton and zooplankton are parts of the plankton. The phytoplankton comprises the autotrophic floating beings: cyanobacte

Bque, what is working of heart

what is working of heart

Body musculature - locomotion, Body Musculature - Locomotion The layou...

Body Musculature - Locomotion The layout of the muscle layers surrounding the coelom is necessarily the same in all the major classes of Annelida. The body wall has a layer of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd