Mrna levels and rna species, Biology

You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mutations, that lead to differential expression of the mutated gene in different tissues. For a variety of reasons, you cannot, or do not wish to use micro-array technology. So, you choose to start by measuring by some other means the mRNA levels in a variety of tissues.

What is a good combination of techniques that will allow you to rapidly quantify the amounts of multiple RNA species and to give some indication of the amounts of specific messages (transcripts of genes)? You do have the ability to do many types of PCRs and also have an assortment of DNA probes that are known to effectively hybridize to regions of mRNAs that may contain the mutated regions of interest.

 

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