Mrna levels and rna species, Biology

You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mutations, that lead to differential expression of the mutated gene in different tissues. For a variety of reasons, you cannot, or do not wish to use micro-array technology. So, you choose to start by measuring by some other means the mRNA levels in a variety of tissues.

What is a good combination of techniques that will allow you to rapidly quantify the amounts of multiple RNA species and to give some indication of the amounts of specific messages (transcripts of genes)? You do have the ability to do many types of PCRs and also have an assortment of DNA probes that are known to effectively hybridize to regions of mRNAs that may contain the mutated regions of interest.

 

Posted Date: 3/4/2013 8:14:21 AM | Location : United States







Related Discussions:- Mrna levels and rna species, Assignment Help, Ask Question on Mrna levels and rna species, Get Answer, Expert's Help, Mrna levels and rna species Discussions

Write discussion on Mrna levels and rna species
Your posts are moderated
Related Questions
Explain the Toxicity of Folate? There is no evidence to suggest that it is possible to consume sufficient natural folate to pose a risk of toxicity. However, this clearly does

What  is digestion? The  ingested food material  is  broken down  into smaller constituents which  are assimilable  by  the  blood.  The  process  through which  the major nutr

organism that can not exist in the presence of oxygen ?

Q. Chemical Agents Available for Sterilization? Chemical Agents: these include the following: Alcohols: ethyl alcohol, isopropyl alcohol, trichlorobutanol Aldehydes: f

Mechanical Prosthetic Valves :  These patients should have lifelong anticoagulation. In the absence of anticoagulation systemic embolism and strokes have been reported to be 5-50

The oxidation of sugar in the cell of higher organisms takes place in the ?

Characters of vitamins: Vitamins are organic substances. They are micronutrients and are required in small quantities. They are not synthesised in the body and therefo

Alkaline slant - Observation for Carbohydrate Utilization Pattern Test Alkaline slant (red) and acid butt (yellow) with or without gas production - This indicates fermentation

Aeolian Wind transported materials constitute this category. This can be further divided into dunes or loess. Dunes are found in thr& types of situations. Fitly, these may occu

ALIMEN T A R Y CANAL Endodermal in origin. Long, 6-9 metres. In herbivorous more long than carnivorous.