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A genomic DNA library is made from the genomic DNA of an organism. For instance, a mouse genomic library could be made by digesting mouse nuclear DNA with a restriction nuclease to make a huge number of several DNA fragments but all with identical cohesive ends.
Figure: A simple method of DNA cloning using a plasmid vector
The DNA fragments would then be ligated into linearized plasmid vector molecules or into an appropriate virus vector. This library would hold all of the nuclear DNA sequence of the mouse and could be searched for any ceratin mouse gene of interest. Each clone in the library is called as a genomic DNA clone. Not each genomic DNA clone would hold a complete gene since in various cases the restriction enzyme will have cut at least once within the gene. Thus some clones will have only a category of a gene.
Metamerism oe Segmentation In the early coelomates the coelom was not divided into segments, the whole body cavity was a single space. Hence body movements were not precise.
Osseus defects around implants. They are classified into four main groups. Group I defects: These demonstrate moderate horizontal bone loss with a minimal intrabony component
Explain Indications for Surgery of Total Anomalous pulmonary Venous Connection ? Once TAPVC is diagnosed in a neonate with symptoms, operation should be undertaken immediately.
Risk analysis- Definiton of Food Food : Any substance, whether processed, semi- processed or raw which is intended for human consumption, containing drinks, chewing gu
Oxidation Some enzymes which have the sulfhydryl (-SH) group in the catalytic site are very sensitive to oxidation. Due to oxidation of the -SH group by aerial oxygen
CARBOHYDRATE METABOLISM The endproducts of carbohydrate digestion absorbed into the blood and taken to the liver by hepatic portal vein are four monosaccharide sugar ( C 6
Q. Which are mineral salts? Where in living beings can found mineral salts? Mineral salts are simple inorganic substances made of metallic chemical elements, like sodium, iron,
10 organisms &there mode of nutrition
Seminal plasma in human males is rich in : 1. fructose and calcium 2. glucose and calcium 3. DNA and testosterone 4. ribose and potassium Fructose and Calcium
Define Precautions for Quantitative Determination of Viable Microbes? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Asep
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