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Oxidation
Some enzymes which have the sulfhydryl (-SH) group in the catalytic site are very sensitive to oxidation. Due to oxidation of the -SH group by aerial oxygen or oxidizing agents, a disulfide linkage (-S-S-) forms with the subsequent loss of enzyme activity. The enzyme activity can be restored by the reduction of the enzyme by some reducing agent such as cysteine or glutathione.
Glycolysis generates two ATPs net per glucose whereas gluconeogenesis uses four ATPs and two GTPs per glucose. Thus, if both glycolysis and gluconeogenesis were allows to opera
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