Gene targeting in es cells, Biology

Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.

Posted Date: 9/18/2012 7:52:09 AM | Location : United States







Related Discussions:- Gene targeting in es cells, Assignment Help, Ask Question on Gene targeting in es cells, Get Answer, Expert's Help, Gene targeting in es cells Discussions

Write discussion on Gene targeting in es cells
Your posts are moderated
Related Questions
Driving Force - Mineral Nutrition Let us now find out what is the driving force involved in protein mediated transport. Many membrane transport proteins allow specific solute

Define briefly about the Conjugated Proteins? Conjugated proteins are made up of simple proteins combined with a non- proteinous substance. The non-proteinous substance is know

Protonephridia and Metanephridia Nephridia take place in two major forms - the protonephridium and metanephridium. Protonephridia are found in flat worms. The protonephridial


The state of balance in the internal environment of the body it includes control of the water balance of the blood, body temperature, blood sugar level and blood urea level. Each o

In which meiotic division does the separation of the homologous occur? What are the ploidies of the generated cells after the end of that process? The separation of the homolog

Haemocyanin - Respiratory Pigments This pigment is found in several arthropods and molluscs. Haemocyanins do not consist of haee groups. The metal they consist of is copper, w

Linkage of Health With Development Health, in its comprehensive or holistic sense, refers to the state of completephysical, mental, social and spiritual well being of a person.

Define the Recommended Dietary Allowance of Vitamin E? The requirements for the vitamin E are expressed in terms of tocopherol equivalents (TE) as mentioned earlier - 8 mg fo

Define Steps for prevention strategies of Obesity? The first and most important step for the rapidly progressing developing countries is to collect and organize data from vario