Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Serial Dilution Process - Plate Counts?
Dilution factor is the reciprocal of the dilution used. As number of microorganisms in original sample is not known, the original sample is diluted several times in sterile water blanks to ensure that separate colonies are developed and some colony counts will be in the specified range. The process is called serial dilution. The procedure is shown in Figure. Usually a ten-fold serial dilution is used in the process where 1 ml of the sample is transferred to a 9 ml of the diluent to get 1:10 dilution. 1 ml of this is transferred to another tube of 9 ml of diluent to get 1:100 dilution and so on till the required dilutions are obtained.
Figure: Serial dilution process
It is important that the dilution used should produce a limited number of colonies on plate. When many cells are present, overcrowding of cells may not allow many colonies to develop and may also result in many colonies to fuse. This leads to inaccuracies in count. Similarly, too less colonies are not statistically significant. According to Food and Drug Administration, only those plates having 25-250 colonies should be considered for calculation; but most microbiologists use plates with 30-300 colonies to get the microbial numbers in the sample. Plates getting colonies less than 30 are marked too less to count (TLTC) and getting more than 300 are marked as too numerous to count (TNTC) and are discarded.
Explain the term - Muscle Contraction Headache This is one of the most common headaches. It is also known as tension or nervous headaches. They result from sustained contractio
Q. How can an organism that once underwent contact with an antigen be immunized against future infections by the same agent? This phenomenon is called as immune memory when an
INORGANI C SUBSTANCES They are small, simple, low molecular weight substances which are made of elements other than Carbon and Hydrogen combined together. Inorganic substan
B o vi n e spongiform encephalopathy (BSE) Bovine spongiform encephalopathy (BSE) is a transmissible, neurodegenerative, fatal brain disease of cattle characterized by post
A microbiology student isolated a hypothetical bacterial cell that seems to quadruple every generation. Knowing that binary fission is an exponential function, derive a mathematica
State the Definition of Temperature Temperature may be defined as degree or amount of body heat. It is the balance between heat produced and heat lost by the body. It is meas
Therapeutic diets - Qualitative methods Qualitative methods: This is where you give the individual choices, clear guidelines, menu guidance and supporting information su
Q. Outbreak of Staphylococcal food poisoning? Conditions necessary for outbreak of Staphylococcal food poisoning: • Presence of viable staphylococcal bacteria in the food
what is welfare in biology
Q. Requirements of Glutamine in diverticular disease? Glutamine: Wile specific nutrients that may have an impact on diverticula disease have not been studied as thoroughly as t
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd