Explain the pour plate method, Biology

Explain the Pour Plate Method

Isolated colonies can also be obtained by pour plate method. The method involves mixing of small volume of microbial suspension with molten nutrient agar at 45°C and pouring immediately into sterile petri plate. The microbial suspension should be diluted sufficiently to obtain separate colonies on plating. The pour plate method involves adding specified amount (0.1 ml or 1.0 ml) of the dilution to the sterile petri plate. Twenty to twenty five (20-25) ml of nutrient agar medium kept liquefied in a water bath at 45°-50° C is then added to the sterile plate and mixed with the dilution properly by gentle rotation of plate in a circular motion on the table top.

This results in uniform distribution of microorganisms. Once the agar has hardened, each cell is fixed in place and forms a distinct colony on incubation. Colonies appeared both within the nutrient agar, as well as, on the surface of agar plate. Microbial cells get fixed on solidification of agar and forms individual colonies on incubation. Colonies are present both on the agar surface and embedded in the nutrient medium. Colonies obtained at different dilutions are also highlighted in the Figure. Colonies growing on the surface can be used to inoculate fresh medium for pure cultures. The method can also be used for microbial cells enumeration in the original sample. We will practically try out this technique in the next practical. But here let us now highlight the advantages and disadvantages of this technique.

Posted Date: 6/6/2013 6:52:54 AM | Location : United States

Related Discussions:- Explain the pour plate method, Assignment Help, Ask Question on Explain the pour plate method, Get Answer, Expert's Help, Explain the pour plate method Discussions

Write discussion on Explain the pour plate method
Your posts are moderated
Related Questions
Q. Explain Food borne toxic infections? Ans. The food borne toxic infections, you may recall reading earlier, are caused by the ingestion of a large numbers of ent

Name two possible why the number of live bacteria cell have reached the stationary growth by 60hrs and start to die off after 12hrs?

Q. Differentiate between sterilization and disinfection? 1) Sterilization is defined as freeing the object or substance from all life of any kind. It is the process by which a

Explain in brief Chitinous? A structure which is composed of chitin, a complex carbohydrate composed of linearly arranged Nacetyl-glucosamine units. Chitin is a characteristic

What is Waist-to-Hip Ratio? Waist-to-Hip Ratio (WHR) is defined as the measurement of waist circumference divided by hip circumference. For example, if a person's waist measure

Define the role of Fluorine in Human Body? Fluorine is potentially a toxic element. Its essentiality for humans is not established although the role of fluoride in providing pr

At the end of the ventricular contraction, maximum arterial pressure is attained and blood flow is most rapid. This is correctly termed the: a) Arterial maximum b) Diastolic

Are there living beings without cells? Viruses are measured the only living beings that do not have cells. Viruses are constitute by genetic material (DNA or RNA) enwrapped by

Describe the monomer unit of a protein and how monomer units are assembled into peptides.

What is the best microscope to get a detailed view of the parts inside of a preserved plant cell