Define standard titration for estimation of reducing sugar, Biology

Assignment Help:

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method?

Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Add approximately 8-9 ml standard glucose solution from a burette so that about 40-45 mg of glucose are added to the flask (to reduce 10 ml of Fehling's solution about 50 mg of glucose are required). Heat the flask on wire gauze till it starts boiling. After about 30-40 seconds add 1 ml of 0.2% methylene blue solution. Continue titratation by adding sugar solution from the burette a few drops at a time and try to finish the titration in 1 minute. At the end point the methylene blue turns colourless. The remaining solution is reddish brown. Repeat the titration till concordant values are obtained. Calculate the amount of glucose required to reduce 10 ml of Fehling's solution and standardize the Fehling's solution.


Related Discussions:- Define standard titration for estimation of reducing sugar

Isolating mechanism, In this section we are to discuss the means by which p...

In this section we are to discuss the means by which populations get separated or isolated from each other, first gain the status of'Sub-species and finally evolve mechanisms which

Could you survive on a diet which contained no carbohydrate, Could you surv...

Could you survive on a diet which contained no carbohydrate?   It should be possible to survive without carbohydrate as energy can be obtained from fats and proteins.

Arterial conduits and internal mamnrary artery (ima), Arterial Conduits ...

Arterial Conduits LIMA UMA: anastomosis side-t-side with diaganal8 end-lo-sode with LAD Figure: Arterial conduits Internal Mamnrary Artery (IMA)

What is the futile cycle, What is the futile cycle The futile cycle is ...

What is the futile cycle The futile cycle is avoided because covalent modification, by  phosphorylation, has opposite effects on  the  enzymes concerned with  the  synthesis  a

Ecology, what is ecology?sub divison of ecology?

what is ecology?sub divison of ecology?

Objective of nutrient needs during periods of pregnancy, Define Objective o...

Define Objective of nutrient needs during periods of pregnancy? describe the various physiological changes during pregnancy, describe foetal growth and development and

Characteristics of hormones, CHARACTERISTIC S OF HORMONES - (1)      T...

CHARACTERISTIC S OF HORMONES - (1)      They are regulatory chemicals that control and coordinate functions of different body organs. (2)      Hormones are formed by ductle

What are proteins, What are proteins? How can the protein diversity of livi...

What are proteins? How can the protein diversity of living beings be explained? Proteins are molecules made of sequences of amino acids bound by a peptide bond. The genetic

Do we know what causes mental illnesses or how to treat them, Do we know wh...

Do we know what causes mental illnesses, or how best to treat them? Mental illnesses take various forms: debilitating sadness in depression; uncontrollable repetitive actions i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd