Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

How does the intensity of facilitated diffusion differ, How does the intens...

How does the intensity of facilitated diffusion vary in relation to the concentration of the moved substance? What is the limiting factor? Like simple diffusion facilitated dif

Define the sequence-specific dna binding, Which of the following statements...

Which of the following statements regarding the interactions among sequence-specific DNA binding domains and their recognition sites is incorrect? A. Sequence-specific DNA bind

Why do organisms live in certain places, Why do organisms live in certain p...

Why do organisms live in certain places? Think of that, the temperature dissimilarity in the desert is huge. So in order to survive, the cactus plant decreases heat gain and he

The difference between plasmalogen and glycerophospholipids, What is the st...

What is the structural difference between plasmalogen and other glycerophospholipids? What physiological advantage arises from this structural difference?

Can pathophysiology causes cardiovascular disease, Q. Can Pathophysiology c...

Q. Can Pathophysiology causes cardiovascular disease? Cigarette use activates platelets, increases circulating fibrinogen, increases heart rate, and elevates blood pressure. It

Excretion in cockroach, EXCRETIO N IN COCKROACH- Animal is uricotelic....

EXCRETIO N IN COCKROACH- Animal is uricotelic. Excretory organs are malphighian tubules. 60-150 in 6-8 groups. In each group 10-15. Yellow coloured, blind tube, open in gut

What is wall resistance of plant cells, Q. What is wall resistance of plant...

Q. What is wall resistance of plant cells? Does this resistance make difficult the entrance of water into the cell or facilitate? Turgor Pressure TP, or Wall resistance, is the

What are the major organic molecules for living beings, Q. What are the maj...

Q. What are the major significant organic molecules for living beings? There are many types of organic molecules that are important for the living beings. Particularly importan

Define briefly about the nutrition security, Define Briefly about the Nutri...

Define Briefly about the Nutrition security? Nutrition security can be briefly defined as a balance between biological requirements in energy and nutrients and the quantity and

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd