Define isotope dilution method, Biology

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.

Posted Date: 6/20/2013 5:30:42 AM | Location : United States







Related Discussions:- Define isotope dilution method, Assignment Help, Ask Question on Define isotope dilution method, Get Answer, Expert's Help, Define isotope dilution method Discussions

Write discussion on Define isotope dilution method
Your posts are moderated
Related Questions
Q. What are the Communication process steps?Communication process steps: 1. Rapport Building 2. Finding the Problem 3. Planning 4. Implementation of the Plan 5. Ter

Factors affects oxygen dissociation curve - Carbon dioxide Carbon dioxide lowers the oxygen affinity of haemoglobin even if the pH is kept constant. This effect is due to the

what diseases are caused by trypanosoma and entameoba histolytica

Q. What do you mean by Systematic Zoology ? It contains articles on animals systematic, and papers dealing with cytological attributes of species and higher taxa, distribution p

Use of the Activity Chart Organizing teams and to decide its strength of different operations on mass production work. Establishing teams for maintenance and similar

Personal Protective Equipment These equipments are essentially required during working to protect face, eyes, respiratory system hands and clothes. Additionally in various in

List the five major phases of the cell cycle, and briefly explain what happens during each phase. G1 phase: the cell grows. S phase: DNA is copied. G2 phase: the cell prepares

Acute Myocardial Infarction with Haemodynamic Deterioration :  These patients may require urgent insertion of intra aortic balloon pump (IABP) followed by PTCA or surgery. 50 per

• Cyclins accumulate during the G1, S, and G2 phases of the cell cycle. • By the G2 checkpoint (the red bar in the figure), enough Cyclin is available to form MPF complexes (aggre

Deficency Diseases and Metabolic Disorders Nutrients required for life sustenance are grouped into 6 basic classes, viz. water, carbohydrates, proteins, fats, minerals and vita