Define isotope dilution method, Biology

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.

Posted Date: 6/20/2013 5:30:42 AM | Location : United States







Related Discussions:- Define isotope dilution method, Assignment Help, Ask Question on Define isotope dilution method, Get Answer, Expert's Help, Define isotope dilution method Discussions

Write discussion on Define isotope dilution method
Your posts are moderated
Related Questions
The citric acid cycle also called as the TCA cycle (tricarboxylic acid cycle) and the Krebs cycle or the Szent-Györgyi-Krebs cycle is a series of chemical reactions used by all aer

Do algae reproduce sexually or asexually? There are algae that replicate sexually and there are algae that replicate asexually. In unicellular algae reproduction is usually

Plants and animals The activity of living organisms like lichens, fungi, bacteria, blue green algae, bryophytes is also responsible for weathering.  It is referred to as biolog

1. You are observing adsorption of a virus to the host cell, it uses long leg-like structures, what are they called?

What category do pisces, mammalla, amphibia, reptilia and aves fall into

Q. Class Reptilia identity card. How are they characterized according to examples of representing beings, basic morphology, skin, respiration, circulation, nitrogen waste, thermal

Explain the drawback of protein hydrolysates The drawback of many protein hydrolysates, is that when hydrolysed, most of the food proteins liberate bitter  tasting peptides, wh

why is the cartilage more flexible than bone? in general, why does cartilage take longer to repair than bone?

Define the term - microfilaments and microtubules The cytoskeleton is, in fact, termed as the "bone and muscle" of the eukaryotic cell, and is composed of a network, consisting

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4