Clone (verb), Biology

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.

Posted Date: 8/1/2012 7:19:17 AM | Location : United States







Related Discussions:- Clone (verb), Assignment Help, Ask Question on Clone (verb), Get Answer, Expert's Help, Clone (verb) Discussions

Write discussion on Clone (verb)
Your posts are moderated
Related Questions
Transgenic Animal Over the centuries, animals with new combinations of genes have been produced using conventional breeding methods by means of careful selection of particular


Explain Some food applications of pectin As with other viscous polyanions such as carrageenan, pectin may be protective towards milk casein colloids, enhancing the properties (

How do mineral salts participate in osmotic regulation? Osmotic pressure depends on the number of particles dissolved in a solution and not on the nature of such particles. Min

(a)What do you understand by the term 'monoculture'? (b) What is one disadvantage of a monoculture?   a) 'Monoculture' is the term applied to the growing

Crustaceans - Hormones in Growth and Reproduction Arthropods have a tough and frequently hard exoskeleton made up of cuticle composed of chitin and protein. The cuticle impose

Question: (i) Which species of microalgae are associated with ciguatera fish poisoning? (ii) Name five fish species generally implicated in ciguatera fish poisoning in Mau

Extraoral Examination It includes examining the following basic structures which are related to oral cavity. TMJ: Rule out any tenderness, crepitus, clicking or snapping

What is Archaebacteria ? Archaebacteria is a relatively new grouping that was created in the light of relatively recent biochemical and electron microscopic evidence. These fin

Importance of Forests - Habitat and Food Forests provide habitat, and food as well as protection to wildlife species against extremes of climate and help in balancing carbon d