Bergmann's method of cell plating, Biology

Bergmann's Method of Cell Plating

In this method free cells are suspended in a liquid medium at a density twice the finally desired plating density. Melted agar containing medium of otherwise the same composition as the liquid medium is maintained at 35?c in water bath. Equal volumes of the two media are mixed and rapidly spread out in petri dishes in such a manner that the cells are evenly distributed and fixed in a thin layer (about 1 mm thick) of the medium after it has cooled and solidified.

The dishes are sealed with parafilm. The cells to be followed are marked on the outside of the plate and before the colonies derived from individual cells grow large enough to merge with each other. They are transferred to separate plates. Another popular method for single cell culture is the microchamber technique, developed by Jones et al. (1960). In this method mechanically isolated single cells are cultured in separate droplets of liquid medium. While Jones et al. used sterile microslides and three coverglasses to make microchamber, it is now possible to buy pre-sterilised plastic plates with several microwells. Individual cells are cultured in separate wells each containing 0.25 ml of the liquid medium. The culture requirement of single cells increases with decrease in the plating cell density, and the cell cultured in complete isolation require a very complex culture medium. A simple medium conditioned by growing cell suspension for some time also fulfils the requirements of single cell culture at low density.

Posted Date: 1/24/2013 5:05:28 AM | Location : United States







Related Discussions:- Bergmann's method of cell plating, Assignment Help, Ask Question on Bergmann's method of cell plating, Get Answer, Expert's Help, Bergmann's method of cell plating Discussions

Write discussion on Bergmann's method of cell plating
Your posts are moderated
Related Questions
Food Gathering and Hunting : Food Gathering and Hunting In order to live, man needed to eat and to protect himself from the weather and animals.  For both purposes he found it

There is no splitting of the centromere. The kinetochore fibers contract and the spindle or pole fibers elongate , As a result homologous chromosomes of each pair rather than

Occurrence of Vitamin D Cholecalciferol is synthesized in the skin by the action of ultraviolet light on 7-dehydrocholesterol which is widely distributed in animal fat. Vitami

Coronal Disassembly -If the existing restoration functionally designed well fitting and esthetically pleasing , -The access the pulp chamber during retreatment is better app

Q. What are zoonoses? What are few examples of zoonoses transmitted by birds? Zoonoses are human diseases transmitted by animals. Psittacosis, a bacterial disease, cryptococcos

Neurosyphilis  Symptomatic neurosyphilis, including ophthalmic or otic infection, requires treatment with high doses of aqueous penicillin G IV or procaine penicillin G IM with

Biological catalysts à speed up chemical reactions in the body without being consumed in the process Reduce the activation energy needed for reactants to reach the transit

Adefovir dipivoxil (Hepsera)  This phosphonate nucleoside analog, administered as the oral prodrug, inhibits replication of HBV, including variants resistant to lamivudine. Ade

Alterations occurring in nuts and oilseeds Some oilseeds have acquired a great significance in the large-scale industrial production of edible oils.  You have already studied t

Nucleic Acids Friedrich Miescher  (1868) discovered the presence  of these compound  in protoplasm ,but Altman (1889) introduced the term Nucleic acid. These  acids are the lar