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Which of the following best defines the reason why DNA ligase is needed to complete the replication of internal chromosomal segments?
A. DNA ligase is able to delete the last nucleotide of the oligonucleotide that is left by RNaseH.
B. DNA ligase is able to incorporate nucleotides into the gap that is left by RNaseH and the exonuclease.
C. DNA ligase is able to make the final phosphodiester bond thereby fixing single stranded nicks that are left by DNA polymerase.
D. DNA ligase is able to relieve the tangles that happen ahead of the replication fork.
There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a pl
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