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Why Capsulate Staining is Difficult?
Capsule staining is difficult because capsular material is soluble in water and may be dislodged or removed during vigorous washing. Negative staining can be used to visualize the capsule. Here presence of capsule is shown by mixing the bacteria in a loopful solution containing tiny colloidal suspension of coloured particles e.g. India Ink or nigrosin which cannot penetrate the cell or its capsule. After spreading the mixture in a thin film and air-drying, counter staining of cells is done with simple stains such as safranin, crystal violet etc. Capsule does not accept these dyes so appear as a halo surrounding coloured cells in a dark blue-black background.
Glycogen is a multibranched polysaccharide which serves as a part of energy storage in fungi and animals. In humans the glycogen is recognized and stored primarily in the cells of
CHLAMYDIOSIS Amongst various causes of morbidity and mortality chlamydiosis is recognized as one of the important infectious conditions and emerging as threat to the livestock due
A woman was injured several years ago in a car accident and she was diagnosed with a herniated disc. Can you please explain using the correct anatomical terminology what herniation
Define Disadvantages of Direct Microscopic Count? 1. Small cells are difficult to see under the microscope and may be missed. 2. It gives total count, i.e., both live and de
Researchers (Helle et al., 2004) analyzed rates of twin births in the Sami population of Northern Scandinavia during the eighteenth and nineteenth centuries. They found that (1) a
Fatty acids are synthesized in the cytosol, but acetyl CoA is produce from pyruvate in the mitochondria. Therefore the acetyl CoA must be transferred from the mitocho
Maintenance and Regulation of Peripheral Circulation Pressure receptors (baroreceptors) located in the wall of the internal carotid arteries and in the arch of aorta, when
What is Septal Ablation (TASH/PTSMA) ? This is a nonsurgical interventional treatment. The septal myocardium supplied by 1 st septal branch of left anterior descending artery
These are areas where woody shrubs predominate rather then trees. In regions with a Mediterranean type of climate i .e., hot dry summers and cool wet winters, shrubs grow close tog
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