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Why Capsulate Staining is Difficult?
Capsule staining is difficult because capsular material is soluble in water and may be dislodged or removed during vigorous washing. Negative staining can be used to visualize the capsule. Here presence of capsule is shown by mixing the bacteria in a loopful solution containing tiny colloidal suspension of coloured particles e.g. India Ink or nigrosin which cannot penetrate the cell or its capsule. After spreading the mixture in a thin film and air-drying, counter staining of cells is done with simple stains such as safranin, crystal violet etc. Capsule does not accept these dyes so appear as a halo surrounding coloured cells in a dark blue-black background.
Mineral availability of organic mineral complexes A number of mineral chelates and complexes are available from a variety of manufacturers. A chelate is described as a metal c
Explain the Bulimia Nervosa? Diagnostic Criteria Bulimia Nervosa patients, unlike those of anorexia nervosa with binge and purge subtype, are typically within the normal weight
Explain the OBJECTIVES of history of mart disease? After reading this unit, you should be able to: 1. Understand the importance of Cardio-vascular diseases as the leading cause
Air movements at tropical latitudes Let us now explain air movements at tropical latitudes. The surface air that rushes to fill the equatorial void from the north is deflected
Explain the Bio availability of Vitamin A? By now it is clear that vitamin A is supplied in two forms. One form is retinol, from animal foods such as liver, fatty fish, eggs, a
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Thermodynamics and Cell Shapes Why are protoplasts (cells devoid of cell wall) spherical? Why are most of the unicellular organisms (prokaryotes and eukaryotes) spherical? A s
Define the Energy Cost of Growth? The energy cost of growth has two components: 1) The energy needed to synthesize growing tissues; and 2) The energy deposited in these tis
Pulse Oximetry: Procedure: Pulse Oximetry is a safe and simple method of assessing oxygenation. An advantage is that this method is noninvasive and continuous. Previously,
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