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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
To study the conditions essential for the germination of seeds:- In the diagram below a having seeds on cotton wool with air, warmth, but no water; b has water, warmth, but no
pioneers of developmental biology and their contribution
Demonstration of specific antigen(s): The presence of the viral antigen in the infected tissues and cells can be demonstrated by fluorescent antibody technique (FAT) and
Determine the Food Sources of Pyridoxine? Raw foods contain more of this vitamin than cooked foods. The food sources include: 1. Rich sources: Rice polishing s, wheat bran,
working of pcr
Diagnosis Clinical signs: In most of the cases in initial stages like bacterial infections there is leucocytosis with neutrophilia, which at later stages of the disease may c
Quantitative Methods - Therapeutic diets Quantitative Methods: These are often essential for constructing Lherapeutic diets. The two ways by which this could be done are as
Why do you think the mitochondria contain their own DNA (circular) and have a double membrane?
Classification according to the modes of transmission 1. Direct zoonoses: The direct zoonoses are those zoonoses that are transmitted from an infected vertebrate host to a
Why is rubella during gestation a threat to the fetus? If occurring during gestation rubella is a dangerous disease due to the virus crosses the placenta and contaminates the f
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