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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
As the name shows, the cytoskeleton is responsible for the supporting of the normal shape of the cell; it also acts as a facilitator for substance transport across the cell and for
Q. What is Etiopathology? Who is likely to develop gout? What are the risk factors? Let us find out. Gout is caused when there is over production of uric acid in normal purine
Restriction endonucleases are enzymes which: 1. make cuts at specific positions within the DNA molecule 2. recognize a specific nucleotide sequence for binding of DNA ligase
The left coronary artery arises from the left posterior aortic sinus. It runs to the left behind the pulmonary trunk and appears between left auricle and pulmonary trunk. In that p
Insect killing jar Secure a wide-mouth glass jar with a screw top or single which closes very tightly. Place a wad of cotton in the bottom and cover it with a round piece of ca
Q. Surgical Treatment of mitral stenosis? Available surgical treatment modalities include closed mitral valvotomy, open mitral valvotomy, and mitral valve replacement. With th
HOW ARE THE ORIGIN AND EVOLUTION OF ALGAE..
Define Some Example of Acidic and Basic Buffer? Frequently used example of acidic and basic buffer includes: - Acidic buffer: a mixture of CH 3 COOH (acetic acid) + CH 3 C
Hydrops amnii (Hydramnios) Hydramnios is a rare condition. Excessive accumulation of amniotic fluid can be the result of foetal dysgenesis and agenesis. The increase of amniot
Mechanical Properties of Ti and Ti Alloy as Compared to Bone Titanium tends to seize when in sliding contact with itself or other metal. Titanium based alloys have a high co-e
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