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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
Explain Procedure for Simple Staining of Bacterial Cultures? Now carry out the exercise following the steps given herewith: (1) Prepare thin bacterial smear on clean glass s
A charged particle A exerts a force of 2.39uN to the right on charged particle B when the particles are 12.3 mm apart. Particle B moves straight away from A to make the distance be
Define need of vitamin C during pregnancy period? Vitamin C deficiency has not been shown to affect the course or outcome of pregnancy in humans, although low plasma levels hav
What is adaptive radiation? The Adaptive radiation is the appearance of several other species from one common ancestral species that have spread to various regions or environme
Dissociation and double dissociations Neuropsychologists typically try to design studies that provide evidence of the differential performance of brain damaged and control subj
Q. How does the male gamete penetrate the egg cell? How does the female gamete protect itself from the entrance of more gametes after the entrance of the first sperm cell? The
Q. What are the three fundamental sexual life cycles studied in Biology? Which of them corresponds to metagenesis? Which of them is the human life cycle? Sexual reproduction ma
Why Deficiency of Folate take place? If there is inadequate dietary folate, the activity of both the DNA and the methylation cycles, described above, will be reduced. A decrea
Following are the functions of Insulin: 1) Insulin controls the level of the sugar (glucose) in the blood. 2) Insulin permits cells to use sugar (glucose) for energy. Cells c
Why is ridge mapping required and what is its alternative The selection of the implant diameter apart from the mesiodistal space available also depends on the buccolingual widt
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