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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
What is the Population of Ecology explain? Ecology is also studied at a higher level of organization the population level. In biological terms, a population is defined as a gro
Q. What is the concept of universality of the genetic code? What are the exceptions to this universality? The genetic code is universal because the rules of protein codificatio
Explain Lyme disease The disease - About 70-80% of patients infected by B. burgdorferi develop the characteristic skin lesion, erythema migrans, which occurs at the site of the
In one study of a heavily polluted area near Birmingham, England, a researcher observed a frequency of 87% melanic Biston betlularia. Calculate the frequency of the dominant allele
what microbe is the one that is shaped like a ball with spikes comming out
Q. Nutritional Management for lactose intolerance? Diagnostic tests are available that can give information about the level and activity of the lactase enzyme. Depending on the
What is the secondary structure of a protein? The secondary protein structure is formed by the manner its amino acids interact by the intermolecular bond. These interactions ma
Mating and Fertilisation In all animals sperms are motile and have to move and seek the eggs to fertilise them. For this an aqueous environment is necessary. This need of a li
What is Septa Septa are thin bony plates, which divide the inferior portion of the antrum into sections and may even create separate compartments. A buttress or web formation m
During mitotic anaphase is there separation of homologous chromosomes or separation of identical chromatids? In the anaphase of mitosis the identical chromatids separate and co
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