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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
What is the functional unity of the kidneys? The functional (filtering) unity of the kidneys is the nephron. A nephron is made of afferent arteriole, efferent arteriole, glomer
Define the Fehling's Soxhlet method (Lane-Eynon method)? This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and t
Ethylene - Dormancy Ripening of fruits involves a chain of cellular events like: Rise in the rate of respiration, Breakdown of higher carbohydrates into carboxylic
explain hadzi''s theory with reference to metazoa
When the level of AMP is high, this shows the requirement for more ATP synthesis. AMP stimulates PFK increases the rate of glycolysis and inhibits fructose 1 and 6- bisphosphatase
write breif notes on oogenesis
Q. Carbohydrates requirements for ulcerative colitis? Carbohydrates: They form the easily absorbable source of energy. Bulk-producing vegetables are restricted so as to allow b
Q. What is the pineal gland? The pineal gland also known as epiphysis or pineal body is situated centrally in the head. It secretes the hormone melatonin. A hormone produced at
Explain about the Protein Isolates? Before we begin our discussion on protein isolates, let us first get to know how protein concentrates differ from isolates. Basically, the t
What are the two models that explain Mechanism of Hormone Action on their target?
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