What is cdna libraries, Biology

Assignment Help:

A cDNA library is made by using the reverse transcriptase of a retrovirus  to synthesize cDNA (complementary  DNA)  copies  of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector.  Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.

 


Related Discussions:- What is cdna libraries

Levels of prevention and role of a nurse, LEVELS OF PREVENTION AND ROLE OF ...

LEVELS OF PREVENTION AND ROLE OF A NURSE: Caplan describes the levels of  prevention as follows:  1)   Primary Prevention  It is concerned with  reduction of  incidence

Define the metabolism and respiration of cornea, Define the metabolism and ...

Define the metabolism and respiration of cornea. Metabolism and Respiration of Cornea The cornea requires energy for the maintenance of its transparency. Energy in

Explain the term- horizons, Explain the term- Horizons These processes ...

Explain the term- Horizons These processes take a long time- may be a few thousand years to create a soil. As the time passes, the soil matures and generally becomes deeper and

Effects of long term malnutrition on adults, Effects of long term malnutrit...

Effects of long term malnutrition on adults In adults, once the stored carbohydrates and fats are used up, the body starts using proteins to generate energy. These protei

Explain genetically modified foods, Normal 0 false false fa...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Plant biodiversity, discuss why obelia is considered to be a special organi...

discuss why obelia is considered to be a special organism in zoology

Ringworms (cutaneous mycoses), Ringworms (cutaneous mycoses) Cutaneous...

Ringworms (cutaneous mycoses) Cutaneous mycoses – also called dermatomycoses, occur worldwide and represent the most common fungal diseases in humans. The dermatophytes are th

Compare and contrast changes in carbon, Compare changes in the carbon cycle...

Compare changes in the carbon cycle to that of the nitrogen cycle on human health. Please make a conceptual graphical model for how changing the carbon cycle is likely to impact hu

Left border of the heart, Connect point A and D by line which is convex to ...

Connect point A and D by line which is convex to the left. The left border is formed by the left ventricle except at its upper end which is formed by the left auricle where there i

Define low water permeability across the luminal membranes, A healthy perso...

A healthy person is on a diet that having of very small amounts of water. A. This person will make a large volume of dilute urine with low amounts of dissolved solutes. B. T

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd