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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
Oxidation Some enzymes which have the sulfhydryl (-SH) group in the catalytic site are very sensitive to oxidation. Due to oxidation of the -SH group by aerial oxygen
Discuss about xeno-transplanation Most defects / diseases treated by xeno-transplanation are not genetic in origin so the therapy will have no impact on gene pool in terms of c
Storage Excretion Many insects exhibit a phenomenon known as storage excretion. If excretory products are stored in the body instead of being eliminated, no water is expended
Rejection Reaction For rejection reaction the physiological and biochemical processes are set in the pistil by the recognition reaction specific to the type of pollen that lan
What is the meaning of term - Plasticity The notion of brain plasticity has been of interest to researchers and clinicians alike for decades. The outcome of injury is the resul
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DNA sequencing is the process of reading the nucleotide bases in a DNA molecule. It includes any technology or method which is used to verify the order of the four bases- guanine,
Enzymes utilization in Baking Industry In the baking of bread, the preliminary process involves the mixing of wheat flour (mainly starch and proteins) with yeast and water. Sta
An amino acid is amphiprotic (have both acid and base) o Some are polar, non polar, acidic or basic List of amino acids (red are non-polar, yellow are
The Eukaryotic cells have five several DNA polymerases; α, β, γ, δ or ε. The DNA polymerases contained in replication of chromosomal DNA are α and δ. DNA polymerases β or ε are con
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