What is cdna libraries, Biology

Assignment Help:

A cDNA library is made by using the reverse transcriptase of a retrovirus  to synthesize cDNA (complementary  DNA)  copies  of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector.  Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.

 


Related Discussions:- What is cdna libraries

What is the salivary digestive enzyme, What is the salivary digestive enzym...

What is the salivary digestive enzyme? Which type of food does it digest and into which smaller molecules does it transform the food? The salivary hydrolase is called as saliva

Coevolution of host-parasites, Coevolutionary relationships between parasit...

Coevolutionary relationships between parasites and their hosts can be more complicated than between predators and their prey. As in the case of prey-predator relationship, one can

Determine the term - essential element, Determine the term - essential elem...

Determine the term - essential element It becomes difficult to meet all the conditions so as to establish essentiality. This is particularly so for the elements that are requir

Drawbacks of lamellar model, Drawbacks of Lamellar model This model ...

Drawbacks of Lamellar model This model does not explain the elasticity of PM. Although this model says that PM is elastic but it also states that head of lipid molecules is

ASSINMENT, HISTORY ON CLASSIFICATION IN BIOLOGY

HISTORY ON CLASSIFICATION IN BIOLOGY

Into which classes are mollusc divided, Into which classes are mollusc divi...

Into which classes are mollusc divided? What are some representing beings of each class? The phylum Mollusca is separated into five major classes: pelecypods, or bivalves (Pele

Haccp - what is validation, HACCP - What is Validation Validation   : ...

HACCP - What is Validation Validation   :  That element of verification focused on collecting and evaluating scientific and technical information to determine  if  the HACCP

Ecology, three types of trophic pyramids

three types of trophic pyramids

Define instrument for absorbed radiation - spectrophotometer, Define Instru...

Define Instrument for Absorbed Radiation - Spectrophotometer? In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation

Agarose gel electrophoresis, Agarose gel electrophoresis  is the method to ...

Agarose gel electrophoresis  is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slowe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd