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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
What is recombination frequency? The Recombination frequency, or crossing over rate, is the percentage of recombinant gametes made by crossing over (in relation to the number o
What is the Microflake - T Dehydration? This technique involves the drying of a continuous sheet of foam 20 mm thick on a continuous stainless steel belt. The later is heated f
What is the most excellent identification hypothesis for a plant tissue seen under the microscope having most cells undergoing cell division? The most excellent hypothesis is t
trycocyst
Transposition of the Great Arteries with VSD and Left Ventricular Outflow Tract Obstruction: The presence of such a condition has to be surgically corrected title is differenc
what is skeleton?
How Factors Affecting Energy Expended in Physical Activity? Physical activity as we learnt earlier is the second largest component of daily energy expenditure, after BMR. Howev
Define the General Mortality and Morbidity Risk? Obesity increases the risk of morbidity and mortality. The obese are more prone to developing morbidities or other chronic dise
Define regulation of malice enzyme by starvation & refeeding? ME catalyzes the oxidative decarboxylation of malate to pyruvate and CO 2 simultaneously generating the reduced
Methods to Overcome Incompatibility It has been possible to facilitate the germination of incompatible pollen by extracting pollen wall proteins from compatible pollen and su
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