The Eukaryotic transcription is more difficult than prokaryotic transcription. For example, in eukaryotes the genetic material DNA and therefore transcription is basically localized to the nucleus, where it is separated from the cytoplasm in which translation occurs by the entaamoeboic nuclear membrane. DNA is also available in mitochondria in the cytoplasm and mitochondria utilize a specialized RNA polymerase for transcription. This allows for the temporal regulation of gene expression by the sequestration of the RNA in the nucleus and permits for selective transport of RNAs to the cytoplasm where the ribosomes reside.