Transcribed regions, Biology

Assignment Help:

Transcribed regions

The transcribed regions of the genes contain a number of regulatory elements that control exon splicing, mRNA transfer to cytoplasm translation, mRNA targeting, and mRNA half-life. A vector for transgene expression must contain at least one intron, which is required for the transfer of the mRNA to cytoplasm, although several introns may be present. The intron may be added before or after the cDNA. Alternative splicing is a quite frequent event in higher-vertebrate mRNA to control gene expression and to enhance the diversity of the cellular mechanisms using a limited number of genes. The association of introns and cDNAs often leads to alternative splicing, which prevents the transgene from driving the synthesis of the expected protein. A model gene construct using whey acid protein promoter is shown below:

 

2102_transcribed regions.png

Fig. WAP (whey acidic protein) construct for expression in mammary gland.


Related Discussions:- Transcribed regions

Difference in sympathetic and parasympathetic nervous system, Difference be...

Difference between Sympathetic and Parasympathetic Nervous System - S.NO . SYM P A THETIC PERASYM P A THETIC 1.

Do enzymes act better under basic or acid ph, Q. Do enzymes act better unde...

Q. Do enzymes act better under basic or acid pH? Most enzymes act in pH between 6 and 8, a range that corresponds to the general acidic level of blood and cells. There are enzy

Enzyme creates double bonds, Suppose you treated butter with a fatty acid d...

Suppose you treated butter with a fatty acid desaturase, an enzyme that removes hydrogen from fatty acids and creates double bonds. Please explain what would happen?

How many chambers does the fish heart have, Q. How many chambers does the f...

Q. How many chambers does the fish heart have? The fish heart is a tube made of two consecutive chambers such as one ventricle and one atrium.

Phylum plattyheminthes, what are structural adaptation of flatworm to it''s...

what are structural adaptation of flatworm to it''s enviroment /

Explain infants and preschool children, Explain Infants and Preschool Child...

Explain Infants and Preschool Children Growth? In this unit we will be studying about one of the crucial phases of human growth and development i.e. infancy to preschool years.

How is the concept of chromosome related to the gene, How is the concept of...

How is the concept of chromosome related to the concept of the gene? A chromosome is a DNA molecule. A chromosome may have several dissimilar genes and also DNA portions tha

In the metagenesis of obelia and aurelia, Q. In the metagenesis of Obelia a...

Q. In the metagenesis of Obelia and Aurelia what is the form that produces gametes? What is the form that reproduces asexually? In the metagenesis of some coelenterates, like O

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd