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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Define Procedure for Testing the Presence of Urea in Milk? 1. To 0.5 ml of milk add 0.5 ml of DMAB solution (prepared by dissolving 0.4 gm of p-dimethyl amino benzaldehyde po
Q. Use of Gloves as personal protective equipment? Gloves are task specific. The different types of gloves worn in the dental office are: i) Examination gloves ii) Over o
COMMON RESPIRATORY DISORDERS: Respiration is one of the most vital functions of the body. The purpose of respiration is to provide oxygen ta the body cells and to remove exc
Explain the technology in which transgenic plant was constructed in which seeds that will produce plants for a single crop.
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Describe 5 ways that a microorganisms directly or indirectly affects our lives.
Q. What do you mean by Alpha Taxonomy? This relates to the basic or preliminary classification based almost entirely on external morphology. This can be considered as the empir
What are heterochromatin and euchromatin? Chromatin is uncondensed nuclear DNA, the typical DNA morphology in interphase (the phase of the cell cycle in which the cells is not
Is it more indicated for a geneticist desiring to map the X chromosome of the mother of a given family (the researcher does not have access to her DNA, only access to the genetic m
what is the biological significance of skeleton
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