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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Define Urinary Excretion Test - Thiamin Status? The urinary excretion of thiamin is an index of recent dietary intake of the vitamin. Hence, determination of thiamin in urine s
Which invertebrate groups possess one, three, four or all of the features below? Segmented bodies, hard exoskeleton, jointed legs, compound eyes, three pairs of walkin
What are trophic levels? How many trophic levels can a food chain have? The Trophic levels correspond to positions on a food chain. thus producers always belong to the first tr
What are meninges and cerebrospinal fluid? Meninges are the membranes that enclose and protect the central nervous system (CNS). Cerebrospinal fluid is the fluid that separates
Q. What do you mean by Ventricular Arrhythmias? Considerable disagreement exists regarding the significance of resting ventricular ectopic beats. In study comparing the coronar
Q. Explain Aortic Valvuloplasty? Valvar aortic stenosis accounts for 4-6 per cent of CHD. LV Outflow Tract obstruction eventually leads to LV dysfunction and congestive heart f
what are the sources of infection (human, animal and non-living reservoirs), the routes of transmission (way in which the infection spreads) and different ways in which micro-organ
Explain Personal Risk Factors in CAD? The personal risk factors are the factors that are specific and characteristic for a particular individual and are non-modifiable in natur
what is the importance of lignin in xylem formation
WHAT DOES CYTON HAVE TO DO WITH NEURON
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