Techniques of Plant Tissue Culture

A standard tissue culture laboratory should provide facilities for washing and storage of glass ware, preparation and storage of nutrient media, aseptic manipulation of plant material, maintenance of cultures under controlled temperature, light and humidity. At least two separate laboratories or rooms should be available one for glassware washing storage and media preparation and another to store cultures. Also it is very essential to maintain a sterile environment during plant tissue culture. By following a few simple precautions against microbial contamination you will save valuable laboratory time in repeating experiments. In the following subsections we will discuss

• Initiation of aseptic techniques
• Culture media and
• Culture conditions