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Reference solution:
A proper reference solution contains color reagent plus sample buffer. The difference between the reference and a sample is that the concentration of the assayable substance in the reference solution is zero. The reference tube transmits as much light as is possible with the assay solution you are using. A sample tube with any concentration of the assayable substance absorbs more light than the reference, transmitting less light to the photometer. In order to obtain the best readability and accuracy, the scale is set to read zero absorbance (100% transmission) with the reference in place. Now you can use the full scale of the spectrophotometer. If you use a water blank as a reference, you might find that the assay solution alone absorbs so much light relative to distilled water that the usable scale is compressed, and the accuracy is very poor.
principle and application
Potassium permanganate is prepared from pyrolusite (MnO 2 ) . Pyrolusite is fused with KOH in the presence of atmospheric oxygen or an oxidising agent like potassium nitrate or po
calculate the voltage of the cell mg/mg+//cd2+/cd at 250c.when[cd2+]=7*10-11 M,[mg2+]=1.0M and standerd electrode potential=1.97V.
general methods of preparation of ethanol
On mixing acetone and chloroform, a reduction in total volume occurs. What kind of deviations from ideal behavior for solutions is given in this case and why?
Fe3+ + e ------> Fe2+
The type of orbitals present in Fe is : (1) s (2) s and p (3) s, p and d ()s, p, d, and f Ans: s, p and d
What are two factors that contribute to the acidity of 2-naphthol
merits
Marine lipids Marine lipids originate from the liver of lean white fish such as cod, the body of oily fish such as mackerel, and the blubber of marine mammals such as seal. T
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