Reagent for separation of amino acid by paper chromatography, Biology

Assignment Help:

Define Reagent for Separation of Amino Acid by Paper Chromatography?

The following reagents will be required for carrying out the experiment:

1. Amino acid solution-

Weigh abour 3-4 mg of each amino acid and dissolve it in dil HCl (1-2 drops). Make the volume upto 10 ml.

2. Chromatographic solvent

Take a separating funnel. To this add 500 ml of freshly shaken mixture of equal volumes of water and butanol. Add 60 ml of glacial acetic acid. Mix. Shake. Discard the lower layer. Take the upper layer.

3. Ninhydrin solution

1% ninhydrin in acetone.


Related Discussions:- Reagent for separation of amino acid by paper chromatography

Synthetic conduits, Synthetic Conduits Dacron, Polytetra fluro ethylen...

Synthetic Conduits Dacron, Polytetra fluro ethylene (PTFE) and polyglycolic acid grafts have extremely poor results and are not used any more.

Explain gluten-free diet, Explain Gluten-free diet Gluten-free diet : ...

Explain Gluten-free diet Gluten-free diet : It is a diet recommended for the patients with gluten enteropathy. Gluten is present in wheat, rye, barley and oats. Thus, foods co

Is transpiration the only way by which leaves lose water, Is transpiration ...

Is transpiration the only way through which leaves lose water? Plants do not only lose water as vapor, as by transpiration. The leaves also lose liquid water by a phenomenon c

Left atrio-ventricular, The left atrio-ventricular aperture in man is guard...

The left atrio-ventricular aperture in man is guarded by

Explain the importance for a hypothesis, What's the difference between theo...

What's the difference between theory and hypothesis and explain the importance for a hypothesis to be testable and falsifiable in order for the scientific method to be applied?

Define changes in body composition due to microgravity, Define Changes in B...

Define Changes in Body Composition due to Microgravity? Major stress in space is microgravity. Microgravity refers to an environment in which there is very little net gravitat

Rate of conversion of atmospheric nitrogen into ammonia, The biological con...

The biological conversion of atmospheric nitrogen into ammonia in the group of plants known as legumes (peas, beans lentils and clover etc.) is achieved by the action of soil bacte

Determine the biological diversity of an ecosystem, Is monoculture a system...

Is monoculture a system that contributes to great biological diversity of an ecosystem? Monoculture means that in a large area a single crop (only single species of plant) is c

Photochemical stage of the photosynthesis process, Q. What are the procedur...

Q. What are the procedures of the photochemical stage of the photosynthesis process? Photolysis of water, with liberation of molecular photophosphorylation and oxygen of ADP, w

Structural features of viruses, Structural Features of Viruses Size of...

Structural Features of Viruses Size of Viruses Virus particles vary widely in size. The smallest viruses e.g. virus of foot and mouth disease  measuring about 20  mill mic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd