Define Reagent for Separation of Amino Acid by Paper Chromatography?

The following reagents will be required for carrying out the experiment:

1. Amino acid solution-

Weigh abour 3-4 mg of each amino acid and dissolve it in dil HCl (1-2 drops). Make the volume upto 10 ml.

2. Chromatographic solvent

Take a separating funnel. To this add 500 ml of freshly shaken mixture of equal volumes of water and butanol. Add 60 ml of glacial acetic acid. Mix. Shake. Discard the lower layer. Take the upper layer.

3. Ninhydrin solution

1% ninhydrin in acetone.