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Radioanalytical methods:
Radioanalytical methods using radiotracers and activation analysis are more sensitive and specific because of ease of detection of nuclear radiation. Being dependent on nuclear property there is complete absence of chemical interferences. Details of isotope dilution analysis (IDA), radiometric titration, radiochromatography and radioimmunoassay including their principles of methodology, applications, advantages and limitations are discussed. The principle of neutron activation analysis, its methodology including instrumentation and typical applications are discussed. Instrumental neutron activation analysis (INAA) has the special advantage of being nondestructive and multielemental besides being blank free and hence very useful for forensic and archaeological studies. However, INAA is suited for the determination of total elemental content only but not suited for speciation studies. A comparative account of NAA and IDA with other trace analysis methods is presented. These methods are preferred in spite of the fact that specially licensed laboratory and radiation safety measures are required.
Merits of atomic fluorescence spectrometry technique: The merit of the AFS methods lies within the greater sensitivity achievable because of low background and interference.
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Geiger Marsden experiment The Rutherford experiment (also known as the Gold foil experiment or the Geiger-Marsden experiment) was an experiment to probe the structure of the at
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There are two type of vitamines 1.water soluble 2.fat soluble water soluble vitamines are B complex and C vitamines vitamine c is soluble in water so dissolve in water and ca
Lassaigne method This is employed to detect halogen, nitrogen and sulphur. Organic compounds are fused along with dry sodium in a fusion-tube and fused mass after extraction wi
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Protein determination In experimental chemistry, biochemistry, biology and biotechnology, it is often vital to know the amount of protein present in a solution. For instance yo
This cycloaddition reaction involves sixteen electrons; hence, it must be suprafacial on one component and antarafacial on the other. Thus, it must be a [14s + 2a] or a [14a + 2s]
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