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Procedure for testing Presence of Vanaspati and Rancidity in Ghee?
Carry out the steps enumerated herewith for conducting this exercise.
For Vanaspati Checking:
1. Take 5 ml of ghee in a stoppered test tube.
2. Add 5 ml of concentrated HCl and shake.
3. Add 5 ml of 2% furfural solution and shake. Observe for the colour in acid layer.
4. Development of pink colour in acid layer indicates the presence of vanaspati.
Rancidity Test:
1. Take 5 ml of melted ghee in a test tube.
3. Add 1 ml of 0.1% Phloroglucinol and observe for development of a colour. If pink colour develops, it is a positive test, i.e., ghee is rancid.
Which one of the following is used as vector for cloning genes into higher organisms ? 1. Baculovirus 2. Salmonella typhimurium 3. Rhizopus nigricans 4. Retrovirus
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