Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Earth''s layers, how are the earth''s layers formed?

how are the earth''s layers formed?

Media and educational environment, Media and Educational Environment: S...

Media and Educational Environment: Some  recent technological developments  in media, have opened up new horizons. Besides, increasing use of media, to support and expand educa

Developmental plasticity, The developing brain is highly plastic in humans....

The developing brain is highly plastic in humans. The downside of this is that the developing human is, compared to other species, practically helpless and without any skills requi

Plaster of paris, Plaster of Paris Secure some plaster of Paris and mix...

Plaster of Paris Secure some plaster of Paris and mix a small amount with water. It must be worked quickly or it will harden whereas being mixed. Place the mixture in moulds an

Science fair , what kind of questions do they ask at a science fair

what kind of questions do they ask at a science fair

Why starch is a non-reducing substance, Starch is a non-reducing substance ...

Starch is a non-reducing substance , as except one carbonyl group, all other carbonyl groups are  involved in glycosidic bond  formation. It may be  hydrolyzed by  boiling with hyd

LP, WHAT ARE THE APPLICATIONS OF LP

WHAT ARE THE APPLICATIONS OF LP

Define the categories of autonomic nervous system, Define the Categories of...

Define the Categories of Autonomic Nervous System Autonomic nervous system is divided into 2 parts - sympathetic and parasympathetic. Sympathetic is a thoracolumbar outflow, para

What are covalent bonds, What are covalent bonds? Covalent bonds are th...

What are covalent bonds? Covalent bonds are those in which electrons are shared by the atoms, and this sharing results in an attraction between the atoms to form a molecule. A

How To Get Back The Super Qualities Of The Brain, Hey There,I Have a Very I...

Hey There,I Have a Very Important Question,A Friend of Mine Watched the Movie The Secret About One Year Ago...And Something Happened To Him,As He Always Knew His Purpouse and Was V

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd