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Define Principle Colorimetric Method for the Estimation of Phosphorous?
The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.
How to make an experimental biter Fit a one-hole stopper carrying a short length of glass tube into the small end of a lamp chimney. Put a little cotton wool in the bottom and
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give the importants segnificant figure in measure quantities
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As glaciers erode the surface over which they move, sets of parallel linear grooves are sometime worn into the rock surface. These grooves are known as: Striations
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