Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Explain somatic mutation theory of ageing, Explain somatic mutation theory ...

Explain somatic mutation theory of ageing Random mutation makes the cells inefficient. An increase in the number of mefficient cells in an organ impairs their functioning. Rand

And the Winner Is..., From the start of this course, we have emphasized the...

From the start of this course, we have emphasized the importance of research in shaping our understanding of human behavior and mental process. Several major studies and social psy

Explain mouth to mouth respiration, Explain Mouth To Mouth Respiration ...

Explain Mouth To Mouth Respiration If no instrument is available, one's own lung may be used as a positive pressure pump. Air may be blown into the lungs of the subject by way

Motion in one dimension, what is the speed and velocity of a car that trave...

what is the speed and velocity of a car that travels 32 meters to the east and 12 meters in the same direction? the total time of travel in 3.0 seconds

Intrusion into the water cycle, Intrusion into the Water Cycle: Water m...

Intrusion into the Water Cycle: Water management is one of  the leading concerns of man  today. The natural water cycle has not been able to compensate for the effects of human

??, what is the best part of the body??

what is the best part of the body??

Role of bile in digestion, Role of Bile in Digestion Bile is produced b...

Role of Bile in Digestion Bile is produced by  liver and stored in gall bladder. During digestion,  the gall bladder contracts  and supplies bile, rapidly to the duodenum throu

Electrochemistry, why we use mercury in the measurement of conductance usin...

why we use mercury in the measurement of conductance using wheatstone bridge principle

Explain fehling''s test, In Fehling's Test The free sugar group (aldehy...

In Fehling's Test The free sugar group (aldehyde or ketone) reduces the Cu  (cupric) ions in  an alkaline environment  to form Cu 10 (cuprous oxide) and the sugar is itself ox

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd