Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Where to find fossils, Where to find fossils In some localities fossils...

Where to find fossils In some localities fossils may be found in stone quarries or where there are rock out- crops. Try to search someone in the community who knows about fossi

Experiment of simple hydraulic press, Simple hydraulic press The princi...

Simple hydraulic press The principle of the hydraulic press is illustrated by the following model. Half fill a cylindrical jar with water. Pour melted paraffin wax on the su

Fst-01, Give reason for the benifits of our technological development not r...

Give reason for the benifits of our technological development not reaching all the strata of our society.

Describe snop coding system, Question 1 Explain the purpose and uses of...

Question 1 Explain the purpose and uses of nomenclature and classification of diseases 2 With a help of an example explain the steps in coding 3 Discuss the medical recor

Define oxytocin hormone - posterior pituitary, Define Oxytocin Hormone - Po...

Define Oxytocin Hormone - Posterior Pituitary Oxytocin is a polypeptide containing 9 amino acids. An 'oxytoxic' agent is a substance that causes the gravid uterus (uterus of a

Factors which decide the br, factors which decide the broad area of Scienti...

factors which decide the broad area of Scientific activity

What is an index, Question 1 What is an index? Explain physician's inde...

Question 1 What is an index? Explain physician's index with the help of examples 2 How to use the Electronic Medical Record for Prior authorization and SOAP technique 3 G

Explain the dialysis, Explain the Dialysis   By now, you must have w...

Explain the Dialysis   By now, you must have well understood the functions of kidney and the implications of abnormal kidney functioning as well. Here in this section, we sh

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd