Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Define the fixed type of macrophages, Define the Fixed Type of Macrophages ...

Define the Fixed Type of Macrophages The fixed types are located permanently in certain organs, which include: Lungs - alveolar macrophages. Liver - kupffer cells.

Geocentric theory , .How did the geocentric theory change over time as incr...

.How did the geocentric theory change over time as increased scientific knowledge led to increased consensus within the scientific community?.

Explain the histotoxic hypoxia, Explain the Histotoxic Hypoxia It is a ...

Explain the Histotoxic Hypoxia It is a type of hypoxia in which due to the action of a toxic agent oxidative enzymes are poisoned hence the tissue cells cannot utilize oxygen s

Explain cutabolic reactions, Explain Cutabolic reactions Cutabolic  re...

Explain Cutabolic reactions Cutabolic  reactions: The cycle provides  a means  for the degradation  of two carbon acetyl residues which are derived from carbohydrates, fatty

Saturated zone of groundwater called, What is the upper surface of the satu...

What is the upper surface of the saturated zone of groundwater called: The Water Table

Determine about the endodynamorphic soils, Determine about the endodynamorp...

Determine about the endodynamorphic soils There are instances where the effect of climate is subdued or masked by the parent material.  Soils formed under such conditions where

Phylum coelenterata, what is the economic importance of phylum coelenterata...

what is the economic importance of phylum coelenterata

A glacier that has neither advanced nor retreated, A glacier that has neith...

A glacier that has neither advanced nor retreated for many years deposits:- end moraine

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd