Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Food borne diseases, Food Borne Diseases: If  the food handler, like ...

Food Borne Diseases: If  the food handler, like the cook, suffers  from some infection and is not careful about washing his hands after defecation,  the germs or eggs of worm

Composition of bile - liver and bile stored in gall bladder, Composition of...

Composition of Bile - Liver and Bile Stored in the Gall Bladder There is a slight difference between the composition of bile produced by the liver and bile stored in the gall b

Protozoa, whats the whole profile for the protozoa fossil

whats the whole profile for the protozoa fossil

State the harmul effects of acidity of soil, State the harmul effects of Ac...

State the harmul effects of Acidity of soil Acidity of soil may have harmful effects on plants due to the effect on enzymatic changes, reduced availability of nutrients like c

Discuss about the life on earth, Discuss about the life on earth We can...

Discuss about the life on earth We can say that all life on Earth is locked in the minerals and by weathering nutrients important to life are made available. Even life in the s

Matter., what is the concepts of matter#

what is the concepts of matter#

Plant Parts, Primary source from plants for humans and animals

Primary source from plants for humans and animals

Define metabolism - physiology of growth and development, Define Metabolism...

Define Metabolism - Physiology of Growth and Development Resting metabolic rate of a newborn is twice as that of an adult/unit body weight basis. Since only colostrums are avai

A famous downslope wind that occurs in north america, A famous downslope wi...

A famous downslope wind that occurs in North America on the East side of the Rocky Mountains is called a: chinook

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd