Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

What is results of recipient operation, What is Results of Recipient Operat...

What is Results of Recipient Operation ? International Society for Heart and Lung Transplantation (ISHLT) maintains data from more than 300 centres starting from 1980. Data fo

Fractures-first aid treatment of localized injuries, Fractures : It is not...

Fractures : It is not our intention to introduce you to many types of fractures that can occur or all the ways a first aider might deal with them. The essential thing is that you

Explain the intercostal muscles, Explain the Intercostal Muscles Look a...

Explain the Intercostal Muscles Look at Figure Can you spot the intercostal muscles? Yes, there are 11 pairs of intercostal muscles. The intercoastal muscles are the muscles

Explain the passive factors or passive soil formers, Explain the Passive Fa...

Explain the Passive Factors or Passive Soil Formers   Passive factors are by and large slow acting factors and include parent material, topography and time.

Explain rancidity, Rancidity:- Formation  of aldehyde due  to  oxidatio...

Rancidity:- Formation  of aldehyde due  to  oxidation  of  unsaturated glycerides.

Broad area, descibe the factors which decidethe broad area of scientific ac...

descibe the factors which decidethe broad area of scientific activity?

Method for all other wastes, For all other wastes i) Burial The spil...

For all other wastes i) Burial The spillage could be buried using any of the following methods: The spillage could be mixed with sand and buried deep. Examples: Iodine

Enviromental, what is the structure of the ecosystem

what is the structure of the ecosystem

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd