Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Acute and chronic effects, Acute and Chronic Effects : The effects of acci...

Acute and Chronic Effects : The effects of accidents due to chemicals are severe and immediate on the human body. These effects are called acute effects and can be traced without

Arthropoda, structure and function of respiratory organs in arthropoda with...

structure and function of respiratory organs in arthropoda with diagram

1, what is a chemical

what is a chemical

Experiments with man in space, Experiments with man in space: We have a...

Experiments with man in space: We have already discussed  the usefulness of space  flights in Blocks 3&4. One is able to make astronomical observations from satellites; get met

The important physico-chemical properties of thiamin, The important physico...

The important physico-chemical properties of thiamin are as follows: a) Thiamin hydrochloride is a white, needle-shaped crystalline substance. b)  It has a characteristic sm

Earth science, why does sedimentary rock form fossils

why does sedimentary rock form fossils

Experiment of simple hydraulic press, Simple hydraulic press The princi...

Simple hydraulic press The principle of the hydraulic press is illustrated by the following model. Half fill a cylindrical jar with water. Pour melted paraffin wax on the su

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd