Principle colorimetric method for estimation of phosphorous, Science

Assignment Help:

Define Principle Colorimetric Method for the Estimation of Phosphorous?

The proteins in the blood are precipitated with the help of trichloroacetic acid. The protein free filtrate containing inorganic phosphorus is then treated with molybdic acid reagent and phosphomolybdic acid is formed. The phosphomolybdic acid is reduced to blue coloured molybdenum blue or hetropolymolybdenum blue (NH4)3{Po4 (Mo03)12}) by 1 amino 2 napthol4 sulfonic acid (ANSA). The intensity of the colour formed is directly proportional to the amount of inorganic phosphorus present. A mild reducing agent like ANSA is employed in order to avoid reduction of the excess of molybdate present.


Related Discussions:- Principle colorimetric method for estimation of phosphorous

Explain fatigue - respiratory adjustments, Explain Fatigue - Respiratory Ad...

Explain Fatigue - Respiratory Adjustments Fatigue means a state of decreased efficiency due to prolonged exertion. It may occur due to sustained muscle contraction. The muscles

Explain the functions of the liver, Explain the Functions of the liver ...

Explain the Functions of the liver Secretion of bile: The liver cells are able to synthesize bile. All constituents of bile are not present in the liver. Liver cells use differ

Protozoa, #question.what is the importance of pritozoa to humans?.

#question.what is the importance of pritozoa to humans?.

Importance of parent materials, Importance of Parent materials Differe...

Importance of Parent materials Different rocks or parent materials placed under similar climatic conditions give rise to soils which are very similar to one another.  On the o

Explain oxidation of fatty acids, Explain Oxidation of Fatty Acids ? F...

Explain Oxidation of Fatty Acids ? Fatty acids released from chylomicrons and VLDL are transferred across cell membranes by passive diffusion, which depends upon the concentra

Disposal of microbial cultures, DISPOSAL OF MICROBIAL CULTURES: After the ...

DISPOSAL OF MICROBIAL CULTURES: After the study is finished microbial cultures should not be left in the laboratory or taken out of the laboratory alive because availability of th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd