Obtaining blood cultures, Biology

Assignment Help:

• Blood cultures are critical in the diagnosis and management of IE.
• Obtain blood cultures before starting antimicrobial therapy whenever possible.
• Intravascular infection leads to constant bacteremia originating from vegetations. Therefore, it is unnecessary to await the arrival of a fever spike or chills to obtain blood cultures.
• There is no significant diagnostic benefit gained from using arterial versus venous blood for culture.
• Always use strict aseptic technique and optimal skin preparation when collecting blood for culture. The recommended antiseptic skin preparation is liberal swabbing with 70 per cent isopropyl alcohol applied in widening circles over a reasonably wide area of skin around the intended site for venepuncture and allowed to dry followed by similar application of an iodophore or tincture of iodine over the same area.
• Blood cultures should be obtained by way of fresh venepunctures and not through indwelling intravascular devices.
• For suspected cases of acute IE obtain atleast two, preferably three sets of blood cultures within 5 to 10 minutes of each other before starting empiric antibiotic therapy.
• For suspected cases of subacute IE draw three separate blood cultures, spaced 30 minutes to 1 hour apart. If these remain negative at 24 hours, draw two further separate cultures.
• The volume of each blood sample drawn should be 20 ml for adults, 1 to 2 ml for neonates, 2 to 3 ml for infants aged 1 month to 2 years, 3 to 5 ml for older children, and 10 to 20 ml for adolescents.
• Each separate blood culture should be divided for inoculation into two bottles. One anaerobic bottle should be included in the total of four bottles inoculated from the two samples to enhance the recovery of certain facultative anaerobes such as streptococci, especially nutritionally variant streptococci.
• If all blood cultures remain negative at 5 days but IE remains likely on clinical grounds, subculture bottles on to chocolate agar plates if the bottles are not held beyond 5 days.
• For optimal processing, the laboratory should be advised that endocarditis is a possible diagnosis and which, if any unusual bacteria are suspected (Legionella species, Bartonella species, HACEK organisms).


Related Discussions:- Obtaining blood cultures

Explain about the classification of fungi, Explain about the Classification...

Explain about the Classification of Fungi? The fungi, as you may recall reading above, is now placed in a separate kingdom called Myceteae. Traditionally, fungi were divided in

Define life cycle of spore forming bacteria, Define Life Cycle of Spore For...

Define Life Cycle of Spore Forming Bacteria? One endospore is formed per bacterial cell. The process of formation of endospore is called sporulation that occurs in an organized

Define regulation and excretion of sodium and chloride, Define Regulation a...

Define Regulation and Excretion of sodium and chloride? Renal excretion and retention of these elements is closely regulated. The total content of body sodium especially the co

Explain catastrophism, Catastrophism Once-popular belief which events in t...

Catastrophism Once-popular belief which events in the history of earth had occurred in the past a sudden events and by processes different from those operating today. The periods

Pair of alleles has different allelesdominance between them, Whenever a pai...

Whenever a pair of alleles has different alleles is there dominance between them? Not in all cases of a gene includes two different alleles is the dominance complete. There are

Exploitation of forests, Exploitation of Forests Exploitation of our f...

Exploitation of Forests Exploitation of our forests for commercial purposes and for food and fodder cannot be stopped completely because of symbiotic relationship between fore

Proteins, assignment on the topic proteins

assignment on the topic proteins

What are Modes of Nutrition, What is Nutrition? Explain types of nutrition?...

What is Nutrition? Explain types of nutrition? Nutrition - Taking Nutrients from environment Nutrients - Substances which are needed for the sources of chemical potential en

Chemical element is the central in the chlorophyll molecule, Q. Which chemi...

Q. Which chemical element is the central in the chlorophyll molecule? The chemical element that is the central in the chlorophyll molecule is magnesium. One atom of magnesium

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd