Method to estimate phytochrome, Biology

Assignment Help:

Method to Estimate Phytochrome

Since there is no chemical test, the only method available to test and estimate phytochrome is the spectrophotometric method. Firstly, the difference in the absorbance between 660 and 730 nm is measured after red light irradiation (Δ OD red, No. 1.). Next, the sample is irradiated with far-red light and again the difference in Δ OD is measured (Δ OD far-red, No. 2). The content of total phytochrome is measured by subtracting the value obtained after red light irradiation from far-red light irradiated value.

2343_Method to Estimate Phytochrome.png

Nowadays special, spectrophotometers are also available which can measure the absorption difference between two wavelengths. These are called dual wavelength spectrophotometers.


Related Discussions:- Method to estimate phytochrome

Injury to adrenal cortex, Injury to adrenal cortex is not likely to affect ...

Injury to adrenal cortex is not likely to affect the secretion of which one of the following? 1. Aldosterone 2. Both Androstenedione and Dehydroepiandrosterone 3. Adrenali

How are parasites classified, Concerning the number of hosts how are parasi...

Concerning the number of hosts how are parasites classified? Parasites that need only one host are known as monoxenous parasites. Parasites that need more than one host for th

Determine the term - coupling factors, Determine the term - coupling factor...

Determine the term - coupling factors The coupling factors, also called the F l ATPase, present on the inner mitochondrial membrane facing the matrix side, are a very complex a

Epidemiological theory - accident causation, Epidemiological Theory - Accid...

Epidemiological Theory - Accident Causation Epidemiological theory of accident causation considers industrial hygiene that concerns environmental issues and may result in sick

What is interdental threaded cleaners, What is Interdental Threaded Cleaner...

What is Interdental Threaded Cleaners The use of floss is recommended around any implant-supported bridge, crown, or bar. Instruct the patient to wrap the floss around the impl

Define precautions for check the presence of rhodamine b, Define Precaution...

Define Precautions for Check the Presence of Rhodamine B? 1. Sample should be clean and dry. 2. Dispose off the paraffin oil carefully, so that it does not contaminate food/

Explain barrier techniques - personal protective equipment, Q. Explain Barr...

Q. Explain Barrier Techniques - Personal Protective Equipment? The use of personal protective equipment (PPE) - clothing, masks, eyewear, and gloves - is part of routine dental

Exercise and medications in bronchial asthma, Exercise Stress or em...

Exercise Stress or emotional upset  Medications Aspirin, non-steroidal anti-inflammatory drugs(NSAIDs), beta- blockers(including eye drops), cholinergic dr

Giant nerve fibres, Giant Nerve Fibres We now know that giant nerve f...

Giant Nerve Fibres We now know that giant nerve fibres take place in the central nervous system of many polychaetes, oligochaetes, insects cephalopod etc. They are broader an

Lactose intolerance - modification of carbohydrate intake, Define Lactose i...

Define Lactose intolerance - Modification of carbohydrate intake? This has been covered under the section on digestion and absorption earlier. We learnt that in case of lactose

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd