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During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?
A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme.
B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).
C. DNA polymerase is not added to the ligation mixture.
D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.
List three human activities which could cause the loss of a species. Human activities which threaten species with extinction are: (i) hunting of individual species, (ii) over-
MOD E OF HORMONE ACTION THROUGH INTRACELLULAR RECEPTORS - Steroid hormones are lipid-soluble and easily pass through the cell membrane of a target cell into the cytoplasm.
A snapdragon plant that bred true for white petals was crossed to a plant that bred for purple petals, and all the F1 had white petals. The F1 was selfed. Among the F2, three other
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State the process of metabotropic receptors Consider a system that contains two neurons and one cardiac SA node cell in a culture dish bathed in normal physiological saline. A
What are the excretory organs in protozoa.
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