Inverse pcr, Biology

Assignment Help:

Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA is digested to completion and fragments ligated under conditions that favour circularization of the fragments; pair of the PCR primers, designed from known sequence, are used to prime PCR from the opposite strands resulting in amplification of fragment of unknown sequence.


Related Discussions:- Inverse pcr

What are the chiasms of homologous chromosomes, What are the "chiasms" of h...

What are the "chiasms" of homologous chromosomes seen in prophase I? Chiasms are intersections of two tracts in the form of X. The chiasms seen in prophase I are chromosome

Sedative hypnotics - psychological drug dependence, SED A TIV E HYPNOTIC...

SED A TIV E HYPNOTICS - They depress the activity of CNS. Reduce excitement, give feeling of calm. Higher doses induces sleep. Sleep inducing drugs are also called

Explain ketogenic diet, Explan Ketogenic diet Ketogenic diet:   It is ...

Explan Ketogenic diet Ketogenic diet:   It is occasionally used to facilitate the control of epilepsy. Here, the patient  is  initially  fasted  for  48 hours and thereafter,

Ecosystem, ECOSYSTEM Plants, animals and human beings live in association...

ECOSYSTEM Plants, animals and human beings live in association with a wide variety of other plants and animals. These communities of organisms are not mere ad boc collections of

How can the abundance and diversity of living beings, How can the abundance...

How can the abundance and diversity of living beings in the tropical forests be explained? The biodiversity of these ecosystems can be defined by the great availability of the

Define descending type of paper chromatography, Define Descending type of p...

Define Descending type of paper chromatography? In descending chromatography, the movement or flow of mobile phase is in downward direction. In this technique the solvent is ke

How cells of neoplastic tumors obtain oxygen and nutrients, Q. How do cells...

Q. How do cells of neoplastic tumors obtain oxygen and nutrients and release wastes? In the neoplastic tumors a phenomenon called as angiogenesis occurs. The Angiogenesis is th

Explain about the dessicator, Explain about the Dessicator? A dessicato...

Explain about the Dessicator? A dessicator is used to prevent an item from absorbing moisture. It is able to accomplish this because of a chemical, known as the dessicant, is h

Zoology, General characters of phylum protozoa

General characters of phylum protozoa

ASSINMENT, HISTORY ON CLASSIFICATION IN BIOLOGY

HISTORY ON CLASSIFICATION IN BIOLOGY

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd