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Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA is digested to completion and fragments ligated under conditions that favour circularization of the fragments; pair of the PCR primers, designed from known sequence, are used to prime PCR from the opposite strands resulting in amplification of fragment of unknown sequence.
Define Popularity of Chromatography - Basic Separation Technique? Chromatography is popular because: (a) minute quantities can be analysed qualitatively and quantitatively,
Explain the Altered Glucose Metabolism - Metabolic Response to Injury? Following injury, hypoglycemia commonly occurs and is related to the severity of the stress. In the ebb p
what is cell?
Which is the normal sign of the electric charge among the two sides of the neuron plasma membrane? What is the potential difference (voltage) generated among these two sides? What
CAUSE S OF VARIATION - Effect of environmental conditions Change in the gene pattern like - (a) Random distribution of homologous genes in meiosis (b) Crossing over
elementatr cannal and feeding mechenism
Assortment is a method in which the meiosis produces new combinations of the genetic information. The paternal and maternal chromosomes line up randomly during the process of syna
Define requirements of Zinc during pregnancy period? Zinc, as a mineral, was found to be associated with foetal growth and birth weight. Low plasma zinc was associated with mor
Explain about the Alitame - artificial sweeteners? Alitame (L-α-aspartyl-N-(2, 2, 4, 4-tetramethyl-3-thietanyl)-D-alaninamide), as illustrated in figure, is a sweetener based o
Explain cigrates smoking from lifestyle risk factors ? Smoking is the single most important preventable risk factor for CAD. There is strong evidence that smoking can significa
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