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Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA is digested to completion and fragments ligated under conditions that favour circularization of the fragments; pair of the PCR primers, designed from known sequence, are used to prime PCR from the opposite strands resulting in amplification of fragment of unknown sequence.
Q Which is the biological molecule that holds the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that contr
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