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Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA is digested to completion and fragments ligated under conditions that favour circularization of the fragments; pair of the PCR primers, designed from known sequence, are used to prime PCR from the opposite strands resulting in amplification of fragment of unknown sequence.
Explain Chemical Structure of saponins? Let us briefly understand their chemical nature. Their structures are characterized by the presence of a steroid or triterpene group, i.
CHROMOLIPID Formed by lipid & pigment, eg. carotene, xanthophylls. Lycopene present in tomato & red chilli. Carrot is rich in b-carotene, converted into vitamin A.
Proteins of Plant Origin Vegetable Proteins: Fresh vegetables are not considered to be a very good source of proteins. On fresh weight basis, the average protein content of s
In the case story, Reggie presented with three typical signs of hip fracture-shortening, adduction, and the lateral rotation of the affected limb. What causes these signs? (HINT-th
Determine the Effect of Iodine Deficiency? Iodine deficiency affects all populations at all stages of life, from the intrauterine stage to old age. However, pregnant women, lac
Q. What is the tympanum? In which part of the ear is it located and what is its function? The tympanum (or ear drum) is a membrane located in the middle ear just after the audi
#applications of biotechnology..
Epithelial cells of the kidney proximal tubule ? A. The sodium-glucose co-transporter in the luminal membrane is responsible for the net flux of glucose from luminal space to
Temperature and Concentration of Nutrients Temperature Temperature like salinity remains almost constant in the oceans in contrast to the land or terrestrial ecosystems
Q. What is diabetes mellitus? Diabetes mellitus is the disease caused by action or deficient production of insulin and the consequent high blood glucose level and low glucose u
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