A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer to Eco RI site than Pst I site of the polylinker, nevertheless get rid of the promoter+RBS+Hin insert already into the plasmid. Describe how you would accomplish the cloning by using a series of numbered steps.

Map of plasmid and a human gene

Remember that in the plasmid, there (1) refers to the frequency of such sites in the plasmid plus existing insert. Into the gene’s restriction map, each the relevant restriction sites are specify d when they are present.