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Head stream of Highland brook zone
This is formed by a number of small streams, which originate from a marsh, a spring or a glacier. This is a small shallow zone with an irregular course. It is formed after torrential rain with no pools and has a low water temperature. The only plant life forms are mosses or liverworts. Fish are absent in this zone.
Sally Subcloner wants to clone a fragment into pUC19 (Map above – From New England Biolabs). Her fragment was a protein coding gene of 2.7 kb, and had XhoI sites on either end (and
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