Genomic dna libraries , Biology

Assignment Help:

A genomic DNA library is made from the genomic DNA of an organism. For instance, a mouse genomic library could be made by digesting mouse nuclear DNA with a restriction nuclease to make a huge number of several DNA fragments but all with identical cohesive ends.

175_22.png

           Figure:  A simple method of DNA cloning using a plasmid vector

The DNA fragments would then be ligated into linearized plasmid vector molecules or into an appropriate virus vector.  This library would hold all of the nuclear DNA sequence of the mouse and could be searched for any ceratin mouse gene of interest.  Each clone in the library is called as a genomic DNA clone. Not each genomic DNA clone would hold a complete gene since in various cases the restriction enzyme will have cut at least once within the gene. Thus some clones will have only a category of a gene.

 


Related Discussions:- Genomic dna libraries

What are transcription process and the replication processes, Q What are th...

Q What are the differences and similarities between the transcription process and the replication processes? A DNA polynucleotide chain serves up as a template in the replication (

Phylum platyhelminthes, PHYLUM  PLATYHELMINTHES Definition and Introd...

PHYLUM  PLATYHELMINTHES Definition and Introduction Bilateral and protostominal  organ  grade eumetazoans  without  a body cavity ( acoelomates)  platyhelminthes means fl

CRUSTACEA, Respiratory system of Crustacea ?

Respiratory system of Crustacea ?

How water content influence the rs content, How Water content Influence the...

How Water content Influence the RS content? The yield of RS formed in heat-moisture treatment is closely related to water content, which may be an inherent component of food or

Production of healthcare - contestability and measurability, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain primary structure of proteins, Explain primary structure of protein...

Explain primary structure of proteins The primary structure of a protein having of the order in which amino acids are bonded to one another by a peptide bond.

What are similarities between reptiles and birds, Q. What similarities do r...

Q. What similarities do reptiles and birds share regarding external coverage, reproduction and excretion? Regarding external coverage, birds are similar to reptiles as they pre

Portal circulation, POR T AL CIRCULATION When a vein branches into se...

POR T AL CIRCULATION When a vein branches into set of capillary to distribute blood in some parts, such venous circulation is called portal system. Three types of portal syst

What is the relationship between enzyme, Q. What is the relationship betwee...

Q. What is the relationship between enzyme and vitamins cofactors? Many vitamins are enzyme cofactors that cannot be synthesized by the organism and must be acquired from the d

Cell coat and its functions, CELL COAT (GLYCOCALYX) Present in Proti...

CELL COAT (GLYCOCALYX) Present in Protists and animal cells. Usually made of oligosaccharide side chains of glycolipids and glycoproteins. Cell coat has very charged t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd