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The ultimate aim of somatic gene therapy is to alter the genetic material of living cells which involves transfer of DNA. Genetic material is transferred by various methods like use of viral or non viral vectors. The choice of an appropriate vector depends on several factors like ability of stimulating immune response, duration for which the transgene is expressed, efficiency in targeting the dividing or quiescent cells, efficiency of integration with the host genome without producing safety issues, the length of trangene which can be transported etc (Gould and Favorov, 2003).
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Transplantation - Clonal Propagation The shoots or plantlets multiplied on a medium containing organic nutrients, show poor photosynthetic capability. Moreover, in these pla
Suppose that a portion of double-stranded DNA in the middle of a large gene is being transcribed by an RNA polymerase. As the polymerase moves through the sequence of six bases sho
Define Nutritional Requirements and Recommended Allowances for Proteins and Amino Acids? The FAO/WHO Committee (1973) expressed the protein requirements in terms of egg or milk
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Trypsin Trypsin is secreted by the pancreas in an inactive form trypsinogen. It is activated by enterokinase secreted by the glands in the intestinal wall. As trypsin is forme
Depth and Currents Depth The sea is very deep varying in different regions. Generally life extends to all depths but is confined more to the continental shelf and
Chromosomes are the condensed, fibrillar, self- replicating genetic structures of cells containing the cellular DNA which bears in its nucleotide sequence the linear array of gene
What is the compound that is phosphorylated for ATP formation? What is the resulting compound when ATP liberates energy? ATP, or adenosine triphosphate, is produced after the
Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiol
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