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Freezing by Contact with a Cooled Solid-Plate Freezing
In this method, the product is sandwiched between metal plates and pressure is usually applied for good contact. Plate freezers are only suitable for regular-shaped materials or blocks. When the product has been frozen, hot liquid is circulated to break the ice seal and defrost. Spacers are used between the plates during freezing to prevent crushing or bulging of the package.
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 d
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