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Explain Transfer from Solid Culture to Liquid Media
The steps involved in this technique are included herewith:
1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.
2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.
After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth. For liquid culture, "shake water baths" can be used at appropriate constant temperature.
A solid compound was found to contain 42.11% C, 51.46%O and 6.43% H by weight. The molecular weight of the compound was 341. What is the molecular formula of the compound?
when pollen tube enters the embryo-sac,it has;
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