Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Morphology, Morphology:  It deals with external forms, structure and the re...

Morphology:  It deals with external forms, structure and the relative position of various organs of living beings. Morphology is a branch of bioscience which dealing with the study

Very low density lipoproteins, Triglyceride and cholesterol synthesized in ...

Triglyceride and cholesterol synthesized in the liver are secreted in very low density lipoprotein (VLDL) particles which serve to transport the lipids to the periphery. VLDL form

Explain tocopherols, Explain Tocopherols These are the most widely dist...

Explain Tocopherols These are the most widely distributed antioxidants in nature, and they constitute the principal antioxidants in vegetable oils.  A relatively high proportio

Can you explain nerves, Q. What are nerves? Axons extend all through th...

Q. What are nerves? Axons extend all through the body inside nerves. Nerves are axon-containing structures presenting many axons and covered by connective tissue. The nerves co

Explain about the glycemic index (gi), Explain about the Glycemic Index (GI...

Explain about the Glycemic Index (GI)? In the previous sections, we have studied that some carbohydrates are rapidly digested and absorbed, some are digested slowly while some

What is excessive cantilever, Excessive Cantilever Cantilevers in impla...

Excessive Cantilever Cantilevers in implant dentistry are commonly used especially in the mandibular arch for edentulous patients receiving implant supported prosthesis. Cantil

What is congenital heart operations and interventions, What is Congenital H...

What is Congenital Heart Operations And Interventions Aftercare of the children who have undergone cardiac procedures and surgeries is as important, and in some occasions, more

In which layer of skin are follicles usually found, 1. In which layer of sk...

1. In which layer of skin are follicles usually found? 2. How are sebaceous glands associated with hair follicles? 3. In which layer of skin are sweat glands usually located?

Animal biodiversity, why obwlia is considered to be of special interest in ...

why obwlia is considered to be of special interest in zoologyas an animal showing an intermediate grade of organisation

Nutrition, mode of nutrition in tapeworm

mode of nutrition in tapeworm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd