Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Arctic tundra, The northern most biome on our planet is the arctic tundra. ...

The northern most biome on our planet is the arctic tundra. It is a treeless wet circumpolar band between the polar ice caps and the forests to the south. The predominant plants

Define higher order structure, Which kind of weak interaction can define hi...

Which kind of weak interaction can define higher order structure and in the same time DNA.

Lactation tetany of mares (eclampsia, Lactation tetany of mares (eclampsia,...

Lactation tetany of mares (eclampsia, transit tetany) Hypocalcaemia in mares causes lactation tetany, which is characterized by abnormal behaviour, in coordination and tetany.

What kind of exoskeleton do some molluscs present, Q. What kind of exoskele...

Q. What kind of exoskeleton do some molluscs present? Some molluscs, like gastropods and bivalves secrete an external calcareous carapace (the shell). Calcium carbonate is a fo

Pulsatile perfusion-type of blood pump, Pulsatile Perfusion: Conventional...

Pulsatile Perfusion: Conventional pumps give continuous flow with very little pulsatile property. Pulsatile perfusion is likely to result in better post operative renal and cardi

Cystitis, C y s t i t i s It is the inflammation of urinary blad...

C y s t i t i s It is the inflammation of urinary bladder characterized by frequent painful urination and presence of blood and cells in urine. E t iology:

What are the archenteron and the blastopore, What are the archenteron and t...

What are the archenteron and the blastopore? What is the stage of the embryonic development in which these structures are formed? What are the destinations of the archenteron and o

Explain doctrine of signatures, Explain Doctrine of Signatures? Handed ...

Explain Doctrine of Signatures? Handed down from the ancients, and elaborated on by the herbalists, was the 'Doctrine of Signatures', which was based on features that resembled

Explain the obligatory losses of nutrients, Explain the Obligatory Losses o...

Explain the Obligatory Losses of Nutrients? Obligatory losses of nutrient are defined as 'the losses that occur when an individual is put on a diet free of that nutrient'. For

Zoology, main external morphological feature that differentiates platyhelmi...

main external morphological feature that differentiates platyhelminthes from nematodes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd