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Explain Transfer from Solid Culture to Liquid Media
The steps involved in this technique are included herewith:
1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.
2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.
After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth. For liquid culture, "shake water baths" can be used at appropriate constant temperature.
The holoenzyme is denatured by heat at very high temperature. It is found that two polypeptides, although denatured, contains chains that remain together as a unit, while two other
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