Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Female accessory sex organs, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Adenine and thymine in complementary strands dna molecule, A "phosphodieste...

A "phosphodiester bond" links together: Answer the 3' hydroxyl group and the N9 atom of adenine in ATP. The Phosphate group and N9 atom of adenine in ATP. Sequential nucleosides in

Explain syphillis, Syphillis  Parenteral penicillin G remains the drug ...

Syphillis  Parenteral penicillin G remains the drug of choice for treating all stages of syphilis. Primary, secondary or latent syphilis known to be of less than one year's dur

World conservation strategy proposes various steps , WORL D CONSERVATION S...

WORL D CONSERVATION STRATEGY PROPOSES THE FOLLOWING STEPS - 1.       Preservation of critical habitats. 2.       Protection of wild life in situ (in natural habitats) as we

Explain the nutritional and functional role of potassium, Minerals :- Potas...

Minerals :- Potassium Food Source      Fruits, vegetables, meats Nutritional Functional role Essential nutrient: Deficiency is rare. Salt substitute: KCl may be

What is coronary disease, What is coronary disease? Coronary disease, o...

What is coronary disease? Coronary disease, or coronary insufficiency, is a disease in which there is total or partial obstruction of one or more of the arteries that irrigate

Control and informative function of organic molecules, Q. What are the few ...

Q. What are the few examples of the control and informative function of organic molecules? Based on genetic information, organic molecules control the entire work of the cell. D

Chromosomes with spindle fiber, Chromosomes with spindle fiber: The kineto...

Chromosomes with spindle fiber: The kinetochore fibers of spindle attach to the kinetochore region (specialized area in centromere) of chromosome, and align them at the equator of

Plant might be adapted to a hot dry environment, Give three ways in which a...

Give three ways in which a plant might be adapted to a hot dry environment. A plant might be adapted to a hot dry environment by having a decreased leaf area, waxy cuticle to

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd