Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Complete blood count, One may find leucocytosis with predominant polymorpho...

One may find leucocytosis with predominant polymorphonuclear cellular response in patients of ARF in presence of acute some throat. Throat Culture When patient presents as AR

Analysis of developmental potency of nuclei, Analysis of Developmental Pote...

Analysis of Developmental Potency of Nuclei The instances given above and many other observations and experimental results of this nature provide tough support to the hypothes

History of psychiatric nursing, History of Psychiatric Nursing: In 187...

History of Psychiatric Nursing: In 1873, Linda Richards, developed better nursing care in psychiatric hospitals, and organized nursing services and educational programmes  in

What is diffusion, Diffusion is the spreading of substance molecules from a...

Diffusion is the spreading of substance molecules from a region where the substance is more concentrated to other region where it is less concentrated. For example, during the boil

Describe about transposition of the great arteries, Describe about Transpos...

Describe about Transposition of the Great Arteries ? Abnormal origin of the great artesies from the ventricular complex. Pulmonary Artery arises from the morphological left ven

Explain repressors , Gene repressor proteins which inhibit the trans...

Gene repressor proteins which inhibit the transcription of particular genes in eukaryotes also exist. They may act by binding either to control parts within the promoter region nea

Lungs - respiration, Lungs - Respiration Lungs can be simple, characte...

Lungs - Respiration Lungs can be simple, characterised by air exchange with surrounding environment by diffusion only. These are called the diffusion lungs and are present in

Gene recombinations, Which of the recombination process(transformation, con...

Which of the recombination process(transformation, conjugation and transduction) would be most likely to occur in the natural environment?

Biochemical function - essential elements, Biochemical Function - Essential...

Biochemical Function - Essential Elements Elements Mg, Mn, K, Ca and Fe are cofactors for many enzymatic reactions. Fe is carrier of electrons in electron transfer chain. Phos

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd