Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Classification of coal by rank, Coal can be classified into following categ...

Coal can be classified into following categories in the order of rank. Peat:  peat is a brown and fibrous mass. It is the first stage of coalification. It is not used as

Explain composition of human milk, Explain Composition of Human Milk? R...

Explain Composition of Human Milk? Research clearly shows that each type of mammalian milk is unique and consists of a highly complex mixture of organic and inorganic compounds

What is the change in allele frequency in a population, Microevolution is: ...

Microevolution is: the change in allele frequency in a population. Cannot occur if genetic equilibrium is reached. Results in the formation of a new species. Is influenced by mutat

Determine factors affecting zn absorption - micro minerals, Determine Facto...

Determine Factors affecting Zn Absorption - Micro Minerals? In the last unit you have studied that absorption of various minerals (bioavailability) is influenced by number of f

Deficiency diseases-selenium and vitamin e deficiency, Selenium and vitamin...

Selenium and vitamin E deficiency Selenium is an essential micro-mineral required for various physiological functions and for immunity against a variety of diseases. It is an i

How many cellular nuclei does the pollen tube of angiosperms, Q. How many c...

Q. How many cellular nuclei does the pollen tube of angiosperms have? What is ploidy of each of these nuclei? The pollen tube explicitly the mature male gametophyte of angiospe

Define transcellular fluid compartment - extracellular fluid, Define transc...

Define transcellular fluid compartment - extracellular fluid? The transcellular fluid is a small compartment that represents all those body fluids which are formed from the tra

Explain the ecological niche modelling, Explain the Ecological niche modell...

Explain the Ecological niche modelling? The geographic range of organisms frequently reflects their fundamental niche, the range of physical conditions where they can live. Qua

Measuring biodiversity, Diversity is defined as the no of species present i...

Diversity is defined as the no of species present in a community termed as "species richness". We can represent species biodiversity at different geographical scales as alpha, beta

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd