Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Define changes associated with the skeletal system - ageing, Define Changes...

Define Changes associated with the skeletal system - Ageing? Skeletal bone loss occurs with ageing and may have serious consequences among the elderly. With ageing, there is so

Aortic valvotomy-surgical techniques, Aortic Valvotomy :  These days ...

Aortic Valvotomy :  These days aortic valvotomy even in neonates and critically ill infants is done under cardio pulmonary bypass. Through a median stemotomy, ascending aorta

What is the endocrine function of the placenta, What is the endocrine funct...

What is the endocrine function of the placenta? The placenta besides being the organ by which the exchange of substances among the mother and the fetus is done also has the fun

What is blood typing?, What is blood typing? The Blood typing is the de...

What is blood typing? The Blood typing is the determination, by means of tests, of the classification of a blood sample concerning blood group systems (specially the Rh system

Races of modern man, RACE S OF MODERN MAN Australoids Negroids ...

RACE S OF MODERN MAN Australoids Negroids Caucasoids Monogoloids American Indian Polynesian All human races have the same number of chromosomes (46) and b

Explain reality theory, Reality Theory Developed in  the 1960s by  Will...

Reality Theory Developed in  the 1960s by  Willian glasser,  a  psychiatrist,  reality  therapists  view human nature in terms of behaviour. They believe that human behaviour i

What is the difference among dna and rna, Concerning the nitrogen-containin...

Concerning the nitrogen-containing bases that participate in nucleotides, what is the difference among DNA and RNA? In DNA nucleotides can be formed of adenine (A), thymine (T)

Medical science in ancient india, MEDICA L SCIENCE IN ANCIENT INDIA D...

MEDICA L SCIENCE IN ANCIENT INDIA Dhanvantri -"God of Medicine". Ashvini Kumar - Famous medical man of vedic times. Susruta - First Indian to study human anatomy;

Evaluation of disabilities, Problem: Evaluation of disabilities due to ...

Problem: Evaluation of disabilities due to occupational diseases (a) Describe the following terms: "impairment", "disability "and "handicap" (b) Show how the evaluation o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd