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Explain Transfer from Solid Culture to Liquid Media
The steps involved in this technique are included herewith:
1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.
2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.
After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth. For liquid culture, "shake water baths" can be used at appropriate constant temperature.
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Define the term - Cholera Cholera is caused by the gram negative, V. cholerae, which is acquired by ingesting food or water contaminated by faecal material from patients or
Explain Observation or Inference required for osazone test? 1. Crystals of different shapes will be seen. Glucose and fructose give needle shaped crystals and galactose gives f
In what ways does over-grazing lead to soil erosion?
Define Role of Buffer in Electrophoresis? Buffer ions have a two-fold purpose in electrophoresis, they carry the applied current, and they fix the pH at which electrophoresis i
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What are the two divisions of the angiosperms? The angiosperms are separated into monocotyledonous and dicotyledonous. (These categories are defined later in this text.) Pla
The trp (tryptophan) operon which is shown in the figure will have five structural genes encoding enzymes for tryptophan biosynthesis with an upstream Ptrp (trp promoter)
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