Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Clinical use of brain imaging in disease management, Determine the Clinical...

Determine the Clinical use of brain imaging in disease management Clinical use of brain imaging in disease management has also grown, driven by broader availability of scannin

Coelomoducts in polyplacophora, Coelomoducts in Polyplacophora In Poly...

Coelomoducts in Polyplacophora In Polyplacophora the coelomoducts divide in the region of coelomostome and the gonadal cavities become closed off from pericardial coelom. The

Explain ribavirin, Ribavirin (Copegus, Rebetol, and others)  Combinatio...

Ribavirin (Copegus, Rebetol, and others)  Combination treatment of HCV with peginterferon alfa and oral ribavirin has produced higher sustained response rates than peginterfero

Explain fats, Explain Fats Fats: As you are already aware,  fats are ...

Explain Fats Fats: As you are already aware,  fats are reservoir  of  calories. In  addition  to carbohydrates,  fats can  also be  an important  source of  needed calories.

Induction in metamorphosis, Induction in Metamorphosis Some of the mo...

Induction in Metamorphosis Some of the morphogenetic changes throughout metamorphosis are found fo be quite independent of hormone action. For instance, generally during meta

Explain the interaction of vitamin a with vitamin e, Explain the Interactio...

Explain the Interaction of Vitamin A with Vitamin E? Of the various nutrients, the interaction of vitamin E, proteins, zinc and iron with vitamin A is of significance. Know? Le

Fertilization - development biology, Fertilization - Development Biology ...

Fertilization - Development Biology Previously you knew the process which leads to the differentiation of the male and female germ cells, the sperm and ova, respectively. In t

Plant responses to light-dark cycles, Plant Responses to Light-Dark Cycles ...

Plant Responses to Light-Dark Cycles Based on their requirement for day length (number of hours of light) for flowering plants have been grouped under three major categories,

Implications of pharmacogenomic research, Problem 1: Describe the funct...

Problem 1: Describe the functions of: a. Glutathione transferases b. Sulfotransferases. -Describe the functions of: a. Glutathione transferases Describe the functions of

Planning and implementation of nursing care - haemophilia, Planning of Nurs...

Planning of Nursing Care Prevent and control  bleeding. Administer replacement therapy of factor VIII and IX as advised.  Educate and support the patient and family.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd