Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Paper points & chemicals removal of gutta percha, Paper points & chemicals ...

Paper points & chemicals removal of gutta percha: -  Flushing action: residual of qutta- percha and sealers chemically solvent by floating pulp chamber with chloroform. -  Chem

Evolutionary advantage of the occurrence of sperm cells, What is the evolut...

What is the evolutionary advantage of the occurrence of sperm cells and larval stage in the life cycle of sponges? The sexual reproduction in sponges, in addition to contributi

Excretory organs, why the excretory organ of prawn is called ''green gland'...

why the excretory organ of prawn is called ''green gland''?

Nursing interventions in rheumatic fever, Nursing Interventions Pr...

Nursing Interventions Primary goals in acute rheumatic fever are:  Control and eradication of the infecting organism.  Prevent cardiac complications.  Relieve

What are the male and the female gonads in humans, Q. What are gonads? What...

Q. What are gonads? What are the male and the female gonads in humans? Gonads are the organs that produce gametes they contain the germ cells that undergo division and generate

Explain about the duration of exercise for diabetics patient, Explain about...

Explain about the duration of exercise for diabetics patient After selecting an exercise, it is very important to know the vigorous nature, duration and timings of the exercise

Microfilaments - cytoskeletal structures, MICROFILAMENTS Discovered ...

MICROFILAMENTS Discovered by Pelvitz. These are smallest cell structure. These are non-living structures. These are solid structures, consists of actin protein (c

Explain the term- loss-of-function mutation, Where would a predicted silent...

Where would a predicted silent mutation have to be situated to actually result in a loss-of-function mutation (and potentially lead to the onset of disease)? A. Intron-exon jun

Tissue distribution and regulation of calcium concentration, Define Tissue ...

Define Tissue Distribution and Regulation of Calcium Concentration? As already discussed, development and preservation of bone mass is quantitatively an important function of c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd