Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Three phases into which the cell respiration is divided, What are the three...

What are the three phases into which the cell respiration is divided? The three phases of aerobic cell respiration are glycolysis, Krebs cycle and respiratory chain (also calle

Behaviour change communication - iron deficiency anaemia, Define Behaviour ...

Define Behaviour change communication - iron deficiency anaemia? In communities that are illiterate and consequently ignorant of the consequences of nutrition disorders and the

What was necessary to develop regulations, Why it was necessary to develop ...

Why it was necessary to develop regulations on the preparation and sale?

Determine the parts of stethoscope, Determine the Parts of Stethoscope ...

Determine the Parts of Stethoscope A stethoscope having of earplugs, binaural pieces, flexible tubing, a stem, and a chest piece. The earplugs are attached to springs made of s

Why does dna twist, Why does DNA twist? If it did not twist, would you...

Why does DNA twist? If it did not twist, would you expect it to fit into the miniscule cell? As we all know, if we join all of the DNA molecules from a person's body end to en

Define role of lipids in controlling gene expression, Define role of Lipids...

Define role of Lipids in Controlling Gene Expression? In the lipogenic pathway, the genes that encode the enzymes of the fatty acid synthase complex are co-ordinately expressed

Define other consideration for dietary management, Define other considerati...

Define other consideration for Nutrient Requirement and Dietary Management Oral intake is generally feasible only during the anabolic phase wherein the patient should be given

Classification scheme of protozoa, Classification Scheme of Protozoa ...

Classification Scheme of Protozoa Phylum Myxozoa Parasites of lower vertebrates especially fish and invertebrates. Phylum Microspora Parasites of inv

Sample titration for determination of iodine number of lipid, Define Sample...

Define Sample titration for Determination of the Iodine Number of Lipids? With a clean dry pipette, accurately measure 0.3 mL of oil into a clean and dry iodination flask (Dupl

Define about the polyphenols, Define about the Polyphenols? We now come...

Define about the Polyphenols? We now come to a group of compounds, which were until recently considered to adversely influence nutrient absorption or giving colour to foods e.g

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd