Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

What is an advantage of using proteins, Plants use proteins, lipids and sta...

Plants use proteins, lipids and starch as seed storage compounds. What is an advantage of using proteins as a storage compound that are not afforded by lipids or starch?

Explain soybean protein isolates, Soybean protein isolates:  Soy protei...

Soybean protein isolates:  Soy protein isolates are the most pure and refined soy protein available.  Isolated soybean proteins (ISP), or  soybean protein isolates, are the mos

Explain the history of darwins finches, Explain the history of Darwin's Fin...

Explain the history of Darwin's Finches? The most well known evidence upon which Darwin based his theory was the distribution pattern of the Galapagos finches. The Beagle's voy

Types of insulin, There are different types of insulin available and prescr...

There are different types of insulin available and prescribed by the doctor according to the need of the patient. Insulin is grouped according to the speed of action in the body i.

Osmosis, everything about osmosis

everything about osmosis

How is genetic engineering used to create bacteria, How is genetic engineer...

How is genetic engineering used to create bacteria capable of producing human insulin? In a production of human insulin by bacteria the human insulin gene is incorporated into

Is the edible part of the onion a fruit, Are fruits always the flesh part o...

Are fruits always the flesh part of the "fruits"? Is the edible part of the onion a fruit? In some so-called fruits the actual fruit is not the flesh part. For instance, the fl

Determine what is the Cell Cycle, The cell cycle undergoes a sequence of ch...

The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.

Isolation techniques, Differentiate between spread plating and streaking?

Differentiate between spread plating and streaking?

Determine the factors that influence manganese absorption, Determine the Fa...

Determine the Factors that Influence manganese absorption? Let us now see which factors influence Mn absorption. Major factors which may influence the absorption of this min

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd