Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

What do you mean by punch cards, Q. What do you mean by Punch cards? Pu...

Q. What do you mean by Punch cards? Punch cards are used when the number of taxa to be keyed out is large. In this type of cards the holes at numbers corresponding to a charact

Geology LAB, Can you guys help with my homework assignment for Geology?

Can you guys help with my homework assignment for Geology?

What is the full chemical equation of photosynthesis, Q. What is the full c...

Q. What is the full chemical equation of photosynthesis? The full chemical equation of photosynthesis is given below: 6 CO 2 + 12 H 2 O + light --> C 6 H 12 O 6    + 6 H 2

Pvcs during recovery, As the heart rapidly slows during recovery from exerc...

As the heart rapidly slows during recovery from exercise, PVCs commonly occur and usually have no clinical significance. This may be a time when metabolic adjustments in the heart

Explain the toxicity of nicotinic acid, Explain the Toxicity of nicotinic a...

Explain the Toxicity of nicotinic acid? Although therapeutically useful in lowering serum cholesterol, administration of chronic high oral doses of nicotinic acid can lead to h

Define instrument for absorbed radiation - spectrophotometer, Define Instru...

Define Instrument for Absorbed Radiation - Spectrophotometer? In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation

Applications of computers in medical and nursing, Applications of Computers...

Applications of Computers in Medical and Nursing Establishment As computers are heavily used for storing and retrieving data the application can be administrative, clinica

Explain composition of amino acid, Explain amino acid composition In te...

Explain amino acid composition In terms of amino acid composition, high radiation doses such as those required for sterilization (e.g. 25-27Kgy), do not change the content of c

Rational in individual patient, Thiazide diuretics have been accepted as th...

Thiazide diuretics have been accepted as the primary foundation of antihypertensive therapy. The basis of this choice is that, apart from their primary hypotensive effect, they enh

Explain the types of fats, Jillian and Michael are in their freshman year a...

Jillian and Michael are in their freshman year at college. The two have been friends since grade school and the two of them enjoy getting together for dinner. Michael typically

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd