Explain three parts of homogenate, Biology

Assignment Help:

Differential centrifugation divides cellular components based on the differences in their size and / or density. A tissue sample was homogenized and three parts (aliquots) of this homogenate were subjected to differential centrifugation using three dissimilar methods as shown below:

Method I: Aliquot 1 was centrifuged at 200,000g for 2 h.

Method II: Aliquot 2 was centrifuged at dissimilar speeds and for variable durations as shown below:

Step 1: 1,000g for 10 min

Step 2: 20,000g for 20 min

Step 3: 80,000g for 1 h

Step 4: 200,000g for 2 h

Method III: Aliquot 3 was processed in the same manner as aliquot 2, except that the sediment and supernatant were divided after each step.

 Cellular components can be obtained as separate fractions from which of these methods?

a.  Only method I 

b.  Only method II

c.  Only method III 

d.  Both methods II and III

 


Related Discussions:- Explain three parts of homogenate

Diversity is good axioms of conservation biology, "Diversity is good" is on...

"Diversity is good" is one of the axioms of conservation biology. However in absentia of historical information, why is noting higher diversity in a system NOT the best way to eval

How do homeotic genes regulate development in drosophila, How do homeotic g...

How do homeotic genes regulate development in Drosophila? Homeotic genes code for regulatory proteins that are thought to control the rate of cell division in various body area

Define interaction of vitamin k with vitamins a and e, Define Interaction o...

Define Interaction of Vitamin K with Vitamins A and E? Excess vitamin A interferes with vitamin K absorption while the a-tocopherol or vitamin E, as we have already studied ear

What do you mean by cold deserts, What do you mean by Cold Deserts? Col...

What do you mean by Cold Deserts? Cold deserts cover a vast area north of the Himalayan ranges forming an ecosystem with exceptionally low temperatures which may reach - 75°C a

Define the process of feeding 0-6 months infant, Define the Process of Feed...

Define the Process of Feeding 0-6 Months Infant? The term exclusive breast feeding is used when all fluids, energy and nutrients are provided by breast milk, with the possible

Venous drainage of the heart, The coronary sinus is the main vein of the he...

The coronary sinus is the main vein of the heart and is about 3 cm. long. It lies in the coronary sulcus at the posterior surface of  the heart in the posterior atrio-ventricular g

Names and uses of the various laboratory tools, What are the names and uses...

What are the names and uses of the various laboratory tools? Tools are beakers, microscopes, tweezers, hot plates, lasers, test tubes, voltmeters, Erlenmeyer flasks, thermomete

Why he literally paralyzed the cilia, Your patient has a respiratory diseas...

Your patient has a respiratory disease that has literally paralyzed the cilia. Explain why this patient would be at an increased risk for a respiratory infection.

Definition of osseointegration in microscopic biology, Q. Definition of Oss...

Q. Definition of Osseointegration in microscopic biology? From a view point of macro and microscopic biology and medicine. Osseointegration of a fixture in bone is defined as t

Hollow restorations for re-lining, A hollow restoration may be fabricated b...

A hollow restoration may be fabricated based on the original form determined by the diagnostic preview. This restoration can be re-lined on completion of the surgical phase of impl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd