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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
steps in urine formation and the organs/tissues involve in such process?
All of the following illustrate why the bile acids are much better suited then cholesterol for use as emulsification agents for fats except: -cholesterol contains a hydroxyl gro
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Cry o Preserved Allografts (Homograft) : As these are not mounted, a free hand suturing in two layers has to be done. The valve is thawed by protocol. The septal muscle i
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Little lipophilic (lipid-soluble) hormones distribute across the plasma membrane and then interact with intracellular receptors in the nucleus/cytosol. The receptor
What is the mode of nutrition in fish,human,amoeba,scorpian & toad ?
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