Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Influence of associated ions, Influence of Associated Ions The second i...

Influence of Associated Ions The second important factor influencing the plant uptake of a given cation is the effects of the ions held in association with it. For instance, as

Explain the general characteristics of honey, A) General Characteristics ...

A) General Characteristics 1) It should be a well-ripened natural product. 2) It should be clear and visually transparent. 3) It should be extracted with the help of a

What is the contraceptive mechanism of the iud, What is the contraceptive m...

What is the contraceptive mechanism of the IUD? The IUD (intrauterine device) is a piece of plastic coated with copper that is inserted within the uterus by a doctor. Copper is

Explain in detail about male reproductive system, Explain in detail about M...

Explain in detail about Male Reproductive System The male reproductive organ consists of the following:  A pair of testes, held in scortum; produces spermatooa and testoster

What is the significance of elastic capsule chromatophore, What is the sign...

What is the significance of Elastic capsule chromatophore? Specialized pigmented cells on surface of the cephalopods that, by changing their shape, expose differing amounts of

Shows evidence to see the in environment abd ecology, Shows evidence of the...

Shows evidence of the ability to see the interaction between environment / ecology and genetics /  evolution Some candidates may recognise that evolution can result in less no

What''s the diffrence between regeneration and repair, What's the diffrenen...

What's the diffrenent between regeneration and repair ?  Regeneration: replacement of the destructed tissue by the same type of tissue  Repair : replacement of the destru

Animal diseases, Animal Diseases - Diseases are common in domestic anim...

Animal Diseases - Diseases are common in domestic animals. Common signs of illness in animals are - 1.      Cessation of rumination. 2.      Drop in milk yield. 3.

Skeletal system - arm bones, ARM BONES - Each arm contain 30 bones as ...

ARM BONES - Each arm contain 30 bones as : humerus in upper arm, radius ulna in forearm, 8 carples in wrist, 5 metacorples  in plam, 14 phalnges in fingers. HUMERU

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd