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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
Influence of Associated Ions The second important factor influencing the plant uptake of a given cation is the effects of the ions held in association with it. For instance, as
A) General Characteristics 1) It should be a well-ripened natural product. 2) It should be clear and visually transparent. 3) It should be extracted with the help of a
osmoregulation in non chordates
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