Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

How different are pteridophytes from bryophytes, How different are pteridop...

How different are pteridophytes from bryophytes regarding substance transport? Pteridophytes are tracheophyte (vascular) plants, i.e., they have tissues specialized in conduct

Explain hypertensive disorders of pregnancy, Explain Hypertensive Disorders...

Explain Hypertensive Disorders of Pregnancy? Hypertension may have been existing before pregnancy. Alternatively, a mother may develop hypertension during pregnancy, a pregnanc

Describe in detail about retina, Describe in detail about Retina The r...

Describe in detail about Retina The retina is a highly complex layer of nervous tissue. The photoreceptors are rods and cones for scotopic and photopic vision respectively. Th

Vascular cambium, Vascular Cambium The vascular cambium usually, if no...

Vascular Cambium The vascular cambium usually, if not always, has a dual origin within the primary tissues; from provascular strands, and from the "ground" meristem tissues be

Explain the food applications of locust bean gum, Food Applications of Locu...

Food Applications of Locust bean gum Locust bean gum has multiple applications in food industry.  Let us focus on a few of the important ones: Locust bean gum has been u

Blood change during exercise, Why does oxygen concentration in the blood de...

Why does oxygen concentration in the blood decrease during exercise? Why does carbon dioxide concentration in the blood change during exercise?

Soil temperature, Soil Temperature Soil temperature is known to influe...

Soil Temperature Soil temperature is known to influence water absorption and ultimately transpiration to a considerable extent. In many plants water absorption is reduced shar

What does acute disease mean, What does acute disease mean? An sharp di...

What does acute disease mean? An sharp disease is which does not last for a longer duration n has no weakness in the body

Investigation of aortic regurgitation by serial testing, Q. Investigation o...

Q. Investigation of aortic regurgitation by Serial Testing? Serial testing is indicated in asymptomatic patients with severe AR and preserved LV function since LV dysfunction m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd