Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Explain uristix benedicts test, Q. Explain Uristix Benedicts test? No s...

Q. Explain Uristix Benedicts test? No sugar/glucose is excreted in the urine in normal individuals. In diabetics, glucose is excreted by the kidneys when the blood sugar levels

5, Air Microbes

Air Microbes

#tzoology, #affinities of ctenophora with platyhelmintheson..

#affinities of ctenophora with platyhelmintheson..

Nucleic acids, Deoxyribonucleic Acid (DNA) - polymer of nucleotides contai...

Deoxyribonucleic Acid (DNA) - polymer of nucleotides containing genetic information that codes for proteins Nucleotide - a monomer of DNA consisting of a ribose/deoxyribose sug

What is the full chemical equation of photosynthesis, Q. What is the full c...

Q. What is the full chemical equation of photosynthesis? The full chemical equation of photosynthesis is given below: 6 CO 2 + 12 H 2 O + light --> C 6 H 12 O 6    + 6 H 2

Geographic speciation, In almost all animal groups, the mode of speciation ...

In almost all animal groups, the mode of speciation is geographic speciation. It is essential that a population which is a prospective new species is geographically isolated from t

Define prevention of iron deficiency, Define Prevention of Iron Deficiency?...

Define Prevention of Iron Deficiency? Iron deficiency anaemia accounts for approximately one-half or more of all the anaemia's seen worldwide. Iron deficiency without anaemia a

Dominance-structure of community , Dominance In each community, a few o...

Dominance In each community, a few over topping species are present in greater bulk. By their greater number or biomass (living weight) the dominant species modify the habitat

Hearing equilibrium, EQUILIBRIU M - Cristae and maculae are concerned ...

EQUILIBRIU M - Cristae and maculae are concerned with equilibrium. 1 .       DYNAMIC EQUILIBRIUM - Cristare detect turning or rotational movement of head ( angular ac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd