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Explain the Turbidity Measurement?
Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).
The sugar carried in the blood is glucose; the normal value of sugar in the human bloodstream is approximately 90mg per 100 bloods. Sugar is absorbed from the small intestines and
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Mechanical removal of Gutta percha - use k file to create channel then removed by H file Remove gutta percha by rotary ( Gates Glidden = GG ) - use suitable size and remo
why is obelia considered to be of special interest in Zoology as an animal showing an intermediate grade of organisation
Explain the Cultural Practices of single crop Continuous cultivation of a single crop over years on the same site causes accumulation of a particular group of microbes, which d
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Q. Chronic complications of diabetes? These occur gradually when the diabetics do not monitor the blood sugar and they are careless about eating, exercise and the medications a
Explain some Do's and Don'ts when working in Laboratory? 1) Always wear a lab coat when working a laboratory. 2) Ensure that no harm is caused to yourself or the people work
FUNCTIONS OF E.R. (1 ) Mechanical support - E.R. participates in formation of cytoskeleton with microfilaments, microtubules and intermediate filaments. (2 )
A drug has a molec. weight of 200. How many grams would you use to make 100 ml of a solution that is isotonic (for man)? Assume that the diluent is distilled water and that the dru
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