Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Define role of leptin in controlling gene expression, Define role of Leptin...

Define role of Leptin in controlling gene expression? Leptin is a protein product (containing 167 amino acids) of the ob gene expressed exclusively in the adipose tissue. This

Definition of counselling, Q. Definition of Counselling? Counselling ha...

Q. Definition of Counselling? Counselling has been defined as a process of helping/enabling a person/people solve certain interpersonal (not able to continue relationship), emo

Explain the fluoride toxicity, Explain the Fluoride Toxicity? Fluoride ...

Explain the Fluoride Toxicity? Fluoride is a cumulative toxin. Ingestion of fluoride 1.0-1.5 mg/L for several years may produce dental fluorosis, i.e. browning and pitting of t

Define disadvantages of using the plate counts method, Define Disadvantages...

Define Disadvantages of Using the Plate Counts Method? 1. It takes time, usually 24 hours or more is required to obtain visible colonies. 2. Microbial count obtained depends

Hydralazine, Hydralazine is a potent arteriolar dilator and markedly increa...

Hydralazine is a potent arteriolar dilator and markedly increases cardiac output in patients with congestive heart failure. However, as a single agent, it has not been shown to imp

Speciation, Ernst Mayr, for whom, species and species problem have been the...

Ernst Mayr, for whom, species and species problem have been the main concern of research, points out that speciation, the multiplication of species, that is division of one parent

Endothermic and exothermic reactions, List out the endothermic and exotherm...

List out the endothermic and exothermic reactions taking place in our body

Definition of probiotics and prebzotics, Definition of probiotics and prebz...

Definition of probiotics and prebzotics? An early definition of probiotics is 'organisms and substances which contribute to intestinal microbial balance'. Later the word 'subst

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd