Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Mycotic aneurysms, They result from septic embolization of vegetations to t...

They result from septic embolization of vegetations to the arterial vasa vasorum or the intraluminal space, with subsequent spread of infection through the intima and outward throu

Interdependance, Give an example of how two organisms are interdependent.

Give an example of how two organisms are interdependent.

Future - development biology, Future - Development Biology Fertilizati...

Future - Development Biology Fertilization in flowering plants is essential for sustaining life on earth. Production of most crops depends on the effectivity of the fertilisat

Lead, Le a d It is common cause of poisoning in cattle. Lead poisonin...

Le a d It is common cause of poisoning in cattle. Lead poisoning in other species is limited by reduced accessibility, more selective eating habits, or lower susceptibility.

Effects on plants of air pollutants, Effects on plants of Air pollutants ...

Effects on plants of Air pollutants SO 2 , O 3 and NO 2 are strong oxidants and can bring about significant changes in plant cell chemistry. The general effects of pollutant

Explain colloidal state, Explain colloidal state Foods contain a high ...

Explain colloidal state Foods contain a high percentage of water in which other nutrients present are dispersed. The existence of the colloidal state was first recognized by T

Define measuring body composition - underwater weighing, Define Techniques ...

Define Techniques for Measuring Body Composition - Underwater weighing? Underwater weighing (densitometry): It applies the Archimedean principle of water displacement by the fu

Define sample preparation for haemoglobin content in blood, Define Sample P...

Define Sample Preparation for Haemoglobin Content in Blood Wipe the index finger or middle finger with a cotton swab soaked in spirit. Prick the finger with a lancet op

Explain about the diabetes mellitus, Explain about the Diabetes mellitus? ...

Explain about the Diabetes mellitus? You are aware that diabetes may be diagnosed as an exaggerated response in blood glucose concentration following ingestion of a fixed amou

Why is a leguminous crop rotation used in agriculture, Why is a leguminous ...

Why is a leguminous crop rotation used in agriculture? Leguminous crop rotation and other crop rotations are used in agriculture because in these plants many bacteria significa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd