Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Explain asteroid impacts, Asteroid impacts: The hypothesis which links cer...

Asteroid impacts: The hypothesis which links certain mass extinction events with the impact of the comet or asteroid, most particularly the mass extinction 65 million years which

Cost due to injury to worker, Cost due to Injury to Worker Medical...

Cost due to Injury to Worker Medical cost - medicine, surgery, hospitalization, extended treatment. Working time lost due to injury or death. Time loss of cowork

Fundamental aspects of protein structure, Can someone describe the fundamen...

Can someone describe the fundamental aspects of protein structure? I'm already familiar with the amino acid structure, I just don't have a clear conceptual understanding of protein

Determine dominant individual fromhomozygous or heterozygous, Why can cross...

Why can crossing of an individual that manifests dominant phenotype with another that manifests recessive phenotype (for the same trait) determine whether the dominant individual i

Kingdom animalia, in what order did the shellfish belong? in what genus did...

in what order did the shellfish belong? in what genus did the shellfish belong? in what species did the shellfish belong?

Define about the excretion of calcium, Define about the Excretion of Calciu...

Define about the Excretion of Calcium? Calcium is excreted approximately in equal amounts in urine and through intestinal secretions. Bile and other secretions into the intest

Explain the properties of good prebiotic, Explain the Properties of Good Pr...

Explain the Properties of Good Prebiotic? Thus, to summarize, we can say that a good prebiotic should have the following properties: Be active at a nutritionally feasibl

Interactions between minerals, Interactions between minerals Copper de...

Interactions between minerals Copper deficiency has been identified as a serious problem for grazing ruminants. A deficiency may be due to low concentrations of Cu in forage a

Describe about the primary prevention - food allergy, Describe about the Pr...

Describe about the Primary Prevention - Food Allergy? Let us further, dwell on measures we could adopt in primary, secondary and tertiary prevention. Current research in primar

Explain genetic relatedness of the aid-giver, Aid-giving behaviour between ...

Aid-giving behaviour between genetically related individuals in animal groups is often observed in nature. This aid-giving behaviour can evolve when the advantages of helping relat

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd