Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Principle of alkaline copper reduction method?
All sugars which contain a free aldehyde group undergo enolization when placed in hot alkaline solution. This property is utilized in the quantitative determination of sugars. The sugar solution is heated with alkaline copper reagent in a Folin's tube. The solution is made to react with arsenomolybdate solution to produce a blue colour. The intensity of this colour is measured colorimetrically.
For blood sugar estimation, proteins are precipitated from the sample and protein-free filtrate is heated with alkaline copper solution and reduction of alkaline copper solution follows. It is then treated with a special arsenomolybdate reagent which forms a blue colour. The colour developed is measured colorimetrically and compared with glucose standards.
To overcome some of the difficulties that arise due to the fading of colour, the more common method used for blood glucose estimation is the modification of the Nelson Somyogi method (Astoor and King, 1954 method). In this method, the reducing effect of reducing agents like glutathione are avoided by putting whole blood in isotonic solution of copper sulphate and sodium sulphate and preventing hemolysis. The red cells are not hemolysed and preserved in an unlaced condition. The glucose diffuses out of the cells while the nitrogenous reducing substances remain in the cells. They are carried along with the precipitate during deproteinization and are removed by centrifugation. The values obtained with this method are closer to true glucose values.
Diagnostic Clues 1) When pallor, decrease in temperature, and numbness are the only complaints, viability of the limb usually not threatened. A condition of pain, pallor,
Q. Fiehes test and Aniline chloride test? Determine the adulteration in the given honey sample by Fiehe's test and Aniline chloride test This activity will help you to: •
Respiratory Care on Admission (First two hours) Patient is incubated and ventilator-dependent. Monitor blood gases hourly and take corrective action immediately.
Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4
Food Gathering and Hunting : Food Gathering and Hunting In order to live, man needed to eat and to protect himself from the weather and animals. For both purposes he found it
Explain Precautions for Estimation of Sucrose by Fehling Soxhlet Method? 1. Maintain proper temperature of the water bath for complete hydrolysis. 2. Add distilled water to
order of the phylum protozoa
Define about the Centrifuge? Centrifuge is an apparatus consisting essentially of a compartment spun about a central axis to separate contained materials of different specific
Which of the following groups is NOT ionizable? Select one: a. Guanidinium b. Imidazole c. Phosphoryl d. Amine e. Aldehyde
Define the Prevention of iron deficiency anaemia? As in the case of vitamin A deficiency, correction and prevention of dietary inadequacy of iron are important sustainable meth
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd