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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
SE X DIFFERENTIATION MECHANISM - Primitive gonads are identical in both sexes upto 6 weeks of gestation pressure. This gonad is known as bipotential primordial gonad. It
A container is filled to a depth of 20.0 cm with water. On top of the water floats a 30.0-cm-thick layer of oil with specific gravity 0.700. What is the absolute pressure at the bo
C h ic k e n infectious anemia (CIA) Chicken infectious anemia virus (CIAV), a member of genus Gyrovirus of the family Circoviridae, is the causative agent of chicken infe
Define Behaviour modification - combat from obesity Problem? It is very important. A heavy parental hand and parent attitude and behaviours towards child's eating, interferes i
HEAR T OUTPUT The amount of blood pumped by heart per minute. Heart beat is 72. Pump out blood is 70 ml. This 72 × 70 = 5040 ml. ( 5 litres) blood is pumped in a minute.
Give the introduction to Congenital Heart Disease ? The past 3 or 4 decades have witnessed a revolution in pediatric cardiac sciences. A number of conditions previously conside
Explain Precautions for Determination of Fungal and Yeast Count? 1. Maintain aseptic conditions. 2. Keep alcohol away from flame. 3. To avoid infections and allergies, ca
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