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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
List er i o si s The disease is caused by Listeria monocytogenes - Gram positive small non spore- forming bacilli seen in soil, silage, sewage and feces of animals and b
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Explain the Marasmus - protein energy malnutrition? Marasmus, the other end of the same spectrum as kwashiorkor, is common in children below the age of 2 years. The characteris
Test of Deep vein Thrombophlebitis Place blood pressure cuff on unaffected calf and inflate until pain is elicited (usually greater than 150 mm Hg). Place blood press
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