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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Protection of protein meals or bypass protein The protein of oil cakes, having higher protein degradability, such as groundnut cake, mustard cake are utilized in the rumen les
Which of the following are features of allosteric inhibition? During the infection, there are many different environments that P. multocida will encounter. In order to cause an inf
Fertilization - Pre-Embryonic Development Ovulation generally takes place between 9th to 14th day after the end of last menstrual period. Fertilization of oocyte initiates dev
State the ways in which (a) all plant cells, (b) some plant cells vary from animal cells. (a) All plant cells have a cellulose cell wall. (b) Some plant c
diff b/w protonephridia and metanephridia
LYSOSOMES Like mitochondrial lysosomes are also typical membrane bound and dense fluid filled sac like cytoplasmic organelles of all eukaryotic cells these however, diffe
Q. What is the function of the vitellus in the vertebrate egg? How are these eggs classified according to the amount of vitellus within them? Vitellus (yolk) is the nutritive m
It arises from a one embryonic cell known as neuroblast Types of neuron: According to number of process¬ (a) Unipolar: Only axon is there. e.g. mesencephalic nucleus. (b)
Heparinisation Blood when allowed to flow out from body to the circuit tubings can get clotted. To prevent this, heparinisation is done. Patient is administered 3 mg
Growth Regulators Besides the major five hormones, polyamines can also exert regulatory control over growth and development at micromolar concentrations. They are widespread a
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