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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Nutrition and Health The effects of the socio-economic and environmental factors in which one is constrained to live will manifest in terms of the nutritional and health statu
In high altitudes is it necessary for the blood to have more or less hemoglobin? In high altitudes the air is rarefied and oxygen concentration is lower than in low altitudes.
Q. How is the yolk sac formed? What is the function of the yolk sac? The yolk sac is formed from the covering of the vitellus by few cells originated from the primitive gut.
Q. Show the Physical criteria of quality for Honey? Colour, crystallization, pH, acidity, water content are some of the criteria used for analysis of honey. These criteria are
Explain Hypertensive Disorders of Pregnancy? Hypertension may have been existing before pregnancy. Alternatively, a mother may develop hypertension during pregnancy, a pregnanc
molecular test protocol for infectious disease and full test procedure and scientific flyer
Pheromones Serving Functions Other than Reproduction Besides sexual responses as described above pheromones mediate several other types of behaviour. Alarm pheromones have bee
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Q. What is Tall T-Wave? It has been reported that tall T-wave during or after exercise indicate inferior wall ischaemia. However, Ellestad reports the findings that tall T-wav
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