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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
How can a baby become infected with (a) gonorrhoea, (b) syphilis (a) During birth, a baby might be become infected with gonorrhoea bacteria as it passes by t
Binding of Pigments, synthetic dye In addition to water, lipids and volatile flavours, food proteins can bind a number of other substances by weak interactions or
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Q. Define Diabetic Ketoacidosis? Diabetic Ketoacidosis (DKA) is one of the acute complications of diabetes mellitus. The name itself implies that there is acidosis (decrease in
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Indications for Surgery : Once diagnosis of constrictive pericarditis is made and confirmed by chest X-ray, ECG, echocardiogram, CT, MRI scan, cardiac catheterisation and angio,
Q How does the excretory system of nematodes work? The metabolic residuals of nematodes are together by two longitudinal lateral excretory channels that open in one single excr
Determine about the diseases Sleep attacks These are brief, often irresistible, episodes of sleep, probably slow wave, NREM, naplike sleep that last about 15 minutes and can oc
Fertilization - Development Biology Previously you knew the process which leads to the differentiation of the male and female germ cells, the sperm and ova, respectively. In t
Endocytosis is the uptake of extracellular macromolecules across the plasma membrane into the cell. The objects to be ingested is progressively enclosed by a small portion of
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