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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Question 1 Write a short note on the following- Structure of DNA. Satellite DNA Transcription. pBR 322 vector Cytokines Microarrays Question 2 What is
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S c inti g r ap h y or nuclear medicine: Scintigraphy was introduced in veterinary medicine in 1970s. It is highly sensitive and can detect bone dama
Explain Ventricular septal defects VSD Chsude Technique ? Ventricular septal defects are closed on cardiopulmonary bypass. Approach is through median stemotomy. Ascending aort
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Is it more indicated for a geneticist desiring to map the X chromosome of the mother of a given family (the researcher does not have access to her DNA, only access to the genetic m
- Oogenesis involves the formation of haploid cells from an original diploid cell, called a primary oocyte, through meiosis. - The female ovaries contain the primary oocyte. The
Tracheoles - Respiration The tracheal system consists of an air filled system of tubes - the tracheal tubes that branch repeatedly until they become fine as capillaries, and
what does ICZN stand for
For convenience, living things are placed into different groups. Taxonomic breakdown of living things comprises the following categories: Family, Class, Genus, Phylum, Order, K
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