Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Name the gas which is used to regulate activity of the heart, In the more h...

In the more highly developed animals, like humans this gas is used to regulate activity of the heart, blood vessels and respiratory system. WORKING MUSCLES PRODUCE A LARGE AMOUNT O

Define effect of deficiency of chromiumin humans, Define Effect of Deficien...

Define Effect of Deficiency of chromiumin humans? Hallmark of marginal chromium deficiency is impaired glucose tolerance. Individuals receiving TPN without chromium have been s

Explain the process of growth factors, Explain the process by which growth ...

Explain the process by which growth factors promote angiogenesis. Add citation or links.

Show the chemical indicators used in sterilization cycle, Q. Show the Chemi...

Q. Show the Chemical indicators used in sterilization cycle? Chemical indicators indicate the presence of certain conditions during the sterilization cycle, such as the presenc

What are the five kingdoms, Q. What are the five kingdoms into which living...

Q. What are the five kingdoms into which living beings are divided? And which group of living being is out of this classification? The five kingdoms of living beings are the ki

How are platelets formed, How are platelets formed? What is the function of...

How are platelets formed? What is the function of platelets? What consequences does the clinical condition known as thrombocytopenia yield? Platelets, also called as thrombocyt

Name the disorder which is subject of ''the elephant man'', The name Joseph...

The name Joseph Merrick, subject of "The Elephant Man", is generally associated with one disorder, though it now seems likely he actually suffered from a different disorder. Name e

What is excessive fat intake, What is Excessive Fat Intake? Although mi...

What is Excessive Fat Intake? Although minimum amount of fat is essential as calculated above but upper limit of fat should be drawn due to its link with an increased risk of d

Prevention - rheumatic fever, Acute GAS pharyngitis should be treated promp...

Acute GAS pharyngitis should be treated promptly by penicillin or other antibiotics. Indian Council of Medical Research (ICMR) recommends that all cases of pharyngitis must receive

Explain the parasympathetic neurons, Which of the following serves as an ac...

Which of the following serves as an actuating signal, or as part of an actuating signal, in a negative feedback system? A. Action potentials in the fibers of the diaphragm musc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd