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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Write the positive ion isoelectronic with neon The positive ion isoelectronic with neon must have 10 electrons and 11 protons, and thus must have an atomic number = 11. This i
Define Derived proteins - Primary Protein Derivatives? Derived proteins are the derivatives of the protein molecule, apparently fonned through hydrolytic changes in the molecu
State about Electric Field Lines Electric Field Lines (lines of force) - show direction of force on a positive test charge from a distribution of charges. The electric field
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A cleavage furrow appears in the middle of the cell, deepens gradually and divides the cell into two. Thus, at the end of second meiotic division, four daughter cells are formed
Process of Regeneration in Planarians Regeneration in planarians has been broadly studied and considerable information regarding this phenomenon is now available. Let us see w
Name three diseases caused by viruses. There are many diseases caused by virus. Virus diseases include colds, influenza, herpes, mumps, measles, chicken pox, rubella, hepati
Which one of the following is not a lateral meristem? 1. Intrafascicular cambium 2. Interfascicular cambium 3. Phellogen 4. Intercalary meristem Intercalary m
Explain Procedure for Spore Staining in a Given Bacterial Culture Carry out the exercise following the steps enumerated herewith 1. Take a clean, non-greasy slide. Prepare b
Q. What is an example of a lab experiment that shows the variation of the photosynthesis effectiveness in relation to different photic energy frequencies to which the reaction is e
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