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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
BLOOD COAGULATION - DEFINITIO N - The property of blood to change from fluid to gel state within a few minutes of its coming in contact with air is called blood coag
A 15 mM solution of potassium chloride is needed for and experiment. The only liquid stock that is a available is a 1 M solution. Calculate how much of the stock solution is needed
Gum Karaya Gum karaya (sterculia gum) is the dried gummy exudate from Sterculia urens Roxburgh and other species of Sterculia (Family: Sterculiaceae) or from Cochlosperm
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can u show the different activities on the topic
Describe about the therapies of illicit drugs do to the brain Despite recent availability of new therapies for addiction, only a small proportion of people who need treatment
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