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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Aplastic Anaemia Aplastic anaemia is a condition in which production of red cells in the bone marrow is reduced. It involves failure to produce adequate red cells, white cell
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what about cytoplasmic sex determination
Tertiary Prevention: In tertiary prevention, emphasis is placed on reducing disability resulting from illness. Tertiary prevention often means long term care. Disability may r
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In patients with left ventricular failure and reduced ejection fractions, the risk of LV thrombus formation and systemic arterial embolization appears to be primarily in patients
Health Education to Hospitalised Child You have unique opportunity for teaching and reinforcing good hygienic care while the child is hospitalised. Clean hair, nails, clothin
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