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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Antimicrobial Prophylaxis - Controversial The need for prophylaxis in breast surgery, herniorraphy and other "clean" surgical procedures has been controversial. Medical Letter
Q. What is the relationship between environmental resistance and the population growth according to the biotic potential curve and the real population growth curve? The differe
Gas Exchange Gill surface area must be large enough to provide adequate exchange of gases. Therefore, highly active fish have largest relative gill area. Figure compares highl
Isonzerization of glucose-6-phosphate Isonzerization of glucose-6-phosphate : This step is catalyzed by phosphoglucoisomerase to form fructose-6-phosphate.
THE CALORIE VALUE - The amount of heat liberated from complete combusion of 1gm food in a bomb caloriemeter (a closed metal chamber filled with O ) is its gross calorific
Explain the Nonpolar Molecular Attractions in basic chemistry? Nonpolar Molecular Attractions : Van der Waals forces and hydrophobic interactions are weak (not as strong
Define Principle for Estimation of cholesterol - Lipids? Zlatkis and associates have evolved a method of quantitative determination of cholesterol based on a reagent containing
Can all the four chambers of the human heart experience systole simultaneously? Describe justifying your answer. What do you call the circulatory fluid in the body of cockroach
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What is Phylum Hemichordata ? The name "hemi"; (meaning "half") provides a clue to these animals, which have some of the features of chordates, and some features that are chara
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