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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. Explain about S-Shaped incision for a mini pedicle flap? The S - shaped incision is indicated where a papilla needs to be developed and was first described by Palacci. This
S e c t i o ned and Formed Meats These are technically speaking restructured products, since they are partially disassembled and then reassembled to form products resembl
The human skeleton has evolved from that of four-legged animals. Unfortunately, the adaptation is far from perfect; thus, our upright posture causes problems like back age and the
What is the structural difference between plasmalogen and other glycerophospholipids? What physiological advantage arises from this structural difference?
Select all that are true or correct regarding a buffer: alternately donating and accepting ions a set of chemicals that work together to resist pH change often a weak acid or base
Explain the interaction of vitamin a with Proteins? Proteins: The protein status of an individual influences vitamin A status and transport because an inadequate protein intake
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Explain Requirements for Simple Staining of Bacterial Cultures Culture: 24 hours bacterial culture of E. coli and Bacillus on nutrient agar or in broth. Reagents: Methylene
Determine the nutritional needs of humans? In the previous unit, we saw how involvement in sport or vigorous activities can affect the body's nutrient needs. In this unit, we w
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