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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is Phospholipids ? Phospholipids : Phospholipids are similar to triglycerides, except that they contain only two fatty acid, and one or both of the fatty acids have
Determine the different types of sensory receptors There are different types of sensory receptors as follows: Visual receptors in the eyes for vision. Auditory receptor
Q. What is the life cycle of the schistosome? Female and Male adult schistosomes live within blood vessels of the human intestines. The females discharge eggs that trespass the
Define the Concept of Health Care? We are aware of the fact that health is a fundamental human right. Thus, it becomes imperative for the State to assume responsibility for the
Oxidised starch Oxidised starch finds a number of uses in the food industry where a neutral tasting, low viscosity 'body builder' is required as in lemon curd manufacture, in s
ENZYME INHIBITION Enzymes are often inhibited by the presence of suitable inhibitors. Much of current drug therapy is based on this. Basically, there are three major cla
excretory product of camel
Q. What do you understand by global warming? The Global warming is the increase in the temperature of the planet due to accumulation of some gases in the atmosphere, especially
Q. Conditions required for Mimicking Pericardial Effusion? In 2DE few conditions can mimic PE. a) Pericardial fat-Mostly localized anteriorly. Absence of fat in the posterio
Explain the De Bakey Type I Dissection? De Bakey Type I Dissection : Initial steps of the operation are same as previously described. Provision for deep hypothermic ci
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