Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Explain about the alzheimer''s disease, Explain about the Alzheimer's Disea...

Explain about the Alzheimer's Disease? Named after the German neurologist who first described it, Alzheimer's disease is the most common cause of progressive dementia, due to t

Exstrophy of the bladder, Exstrophy of the Bladder This is the most c...

Exstrophy of the Bladder This is the most common major congenital defect of lower urinary and genital tract. This is found more frequently in males than in females. Exstr

Explain about rheology of foods, Q. Explain about Rheology of Foods? Rh...

Q. Explain about Rheology of Foods? Rheology is the study of stress and strain or in other words, it is the study of flow and deformation of materials, both liquids and solids,

What do you mean by herbaria and museums, Q. What do you mean by Herbaria a...

Q. What do you mean by Herbaria and Museums? A herbarium is a collection of pressed and dried plants arranged according to some valid system of classification and available for

How do grasslands differ from savannas, How do grasslands differ from Savan...

How do grasslands differ from Savannas? Name the two main categories of plant forms that dominate the desert vegetation.

Ecological pyramids, one disadvantage of the pyramid if a tree and grass ea...

one disadvantage of the pyramid if a tree and grass each count as one organism

How do the products of meiosis i differ from of meiosis ii, How do the prod...

How do the products of meiosis I differ from those of meiosis II? In meiosis I, the offspring cells are haploid but every cell contains two copies of the chromosome because th

Realization of p-d polarity of limb, Realization of P-D Polarity of Limb ...

Realization of P-D Polarity of Limb Realization of P-D polarity of limb expressed in a specified proximo-distal sequence of differentiation of limb pans is controlled through

Gregor mendel''s law of independent segregation, Gregor Mendel's Law of Ind...

Gregor Mendel's Law of Independent Segregation referred to which of the following genetic elements? A. Alleles of two genes that reside on the similar chromosome B. Alleles

Volvocine Protozoa, Why is Volvocine Protozoa not considered an animal?

Why is Volvocine Protozoa not considered an animal?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd