Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Main constituents of the earth''s atmosphere in our time, What are the main...

What are the main constituents of the earth's atmosphere in our time? The present atmosphere of the earth is constituted essentially of molecular nitrogen (N2) and molecular ox

Fresh water-types of water found on earth, Fresh Water Water, a univers...

Fresh Water Water, a universal solvent, invariably contains many soluble salts. In fresh water the total salt content remains under 15 percent. Different types of soluble salts

Explain the character of pulse, Explain the Character of Pulse ? A norm...

Explain the Character of Pulse ? A normal pulse wave has an upstroke and down stroke. Dicrotic notch present on direct arterial traces is impalpable. Figure: Arterial

What are the valvular heart diseases, Q. What are the Valvular heart diseas...

Q. What are the Valvular heart diseases? Valvular heart disease is a common clinical problem. While rheumatic mitral valve disease seen more often in the underprivileged segmen

Classification of aquatic organisms, Classification of Aquatic Organisms ...

Classification of Aquatic Organisms After discussing the common types of the aquatic ecosystem let us now study in brief the ecological classification of aquatic organisms. I

Show the recessive phenotype, A woman who is heterozygous for a particular ...

A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?

What is intermediate acting insulin, Q. What is Intermediate acting insulin...

Q. What is Intermediate acting insulin? Intermediate acting: This type works more slowly than short acting. It works hardest 8-12 hours after injection and still keeps on worki

What is phagocytosis and phagosomes?, Protozoans ingest food by phagocytosi...

Protozoans ingest food by phagocytosis, and consequently, the ingested food is surrounded by a plasma membrane creating a food vacuole, or phagosome. Digestion does not start until

Explain the reproductive cycles of female, Q. Generally how does a male ani...

Q. Generally how does a male animal realize that the female is receptive to copulation? In most vertebrate species with internal fecundation the females have reproductive cycle

Explain the digestibility coefficient - proteins, Explain the Digestibility...

Explain the Digestibility Coefficient? You have earlier learnt that dietary proteins are hydrolyzed to amino acids during digestion. The digestion begins in the stomach by the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd