Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Explain inborn errors of metabolism, Q.  Explain Inborn Errors of Metabolis...

Q.  Explain Inborn Errors of Metabolism? Some of the disorders caused by inborn error of metabolism namely phenylketonuria, tyrosinemia, maple syrup urine disease, homocystinur

Sds page, describe sds page for the proteins

describe sds page for the proteins

Ecology, What is the concept of minimum and maximum way of tolerance

What is the concept of minimum and maximum way of tolerance

What are the two big groups into which cells are classified, Cells are clas...

Cells are classified into eukaryotic or prokaryotic. Prokaryotic cell is that with no a delimited nucleus. Eukaryotic cells are those with nucleus delimited by membrane.

St depression at rest, Q. What is ST depression at rest? As early as 19...

Q. What is ST depression at rest? As early as 1965 Ostrand called attention to the syndrome of short PR interval without accelerated conduction and it's association with ST dep

Byproducts of low digestibility high in nitrogen, Byproducts of the food i...

Byproducts of the food industry, such as coffee residues, fruit pulp and cocoa meal are of low energy value, but are relatively high in nitrogen. In additional, animal excreta tend

What is the spinal cord, What is the spinal cord? Of which elements is the ...

What is the spinal cord? Of which elements is the spinal cord constituted? The spinal cord is the dorsal neural cord of vertebrates. It is the kind of the central nervous syste

Muscle, In a word, what process provides the energy for muscle contraction

In a word, what process provides the energy for muscle contraction

Which germ layer originate liver and the pancreas, Q. From which germ layer...

Q. From which germ layer do the liver and the pancreas originate? What are other organs and tissues made from that germ layer? The pancreas and the liver are originated from th

Describe the structure of alginate, Describe the structure of alginate ...

Describe the structure of alginate The commercially available alginates are white to cream coloured powders. 1% alginic acid solution gives a pH value of 2.9 while it is 2.3 an

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd