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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Cilia and Flagella – Protozoans Cilia and flagella basically have a similar structure and distinction between the two on structural basis does not exist. There is a filament o
Q. How is the nervous system characterized in beings of the phylum Annelida? How can one compare cephalization in annelids to cephalization in platyhelminthes and nematodes? An
If jean-Baptiste de Lamarck was correct, than a. Natural selection would lead to the spread of favorable traits within a population b. Finches that stretched their beaks to access
Gonorrhea Single doses of cefixime (Suprax) orally or ceftriaxone (Rocephin) intramuscularly (IM) are highly effective for uncomplicated anogenital or pharyngeal infection,
how to explain an example from each class of vertebrate
Q. Dietary Recommendations during Diarrhoea? The diet should take into account the normal RDI and various adjustments made to the quantity and quality of the foods to be given.
Tetrasporic Embryo Sacs In this group neither of the meiotic divisions is accompanied by wall formation so that at the end of meiosis all the four haploid nuclei remain in a
Q. How in the respiratory chain do electrons from NADH2 and FADH2 passing through cytochromes liberate energy for the ATP synthesis? What is this ATP synthesis called? NADH2and
Define Aerobic Exercise or Aerobic Energy System? To produce necessary energy, the body uses an aerobic pathway and an anaerobic pathway. Exercise that relies heavily on oxygen
Direction of flow--either towards or away from the transducer (positive or negative Doppler shifts). Timing-instantaneous velocity and direction of flow throughout the various
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