Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Assuming that the two traits assort independent, In holly, serrated leaves ...

In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are

Urolithiasis, Ur o l it h i a s i s The presence of calculi (u...

Ur o l it h i a s i s The presence of calculi (urolith) in the urinary system is termed as urolithiasis, and the disease is characterized by distension of bladder and

Mendel''s yellow peas, A plant grown from one of Mendel's yellow peas is se...

A plant grown from one of Mendel's yellow peas is selfed. Five progeny peas are obtained from this self and they are all yellow. If the original selfed plant had been homozygous, w

BiologicaL METHODS.., GIVE THE SHORT DETAIL OF HE BIOLOGICAL METHODS

GIVE THE SHORT DETAIL OF HE BIOLOGICAL METHODS

What are the functions of the osseous tissue, Q. What are the functions of ...

Q. What are the functions of the osseous tissue? The main functions of the osseous tissue that are to provide structural rigidity to the body and to delineate the spatial posit

What are the fundamental constituents of the cell membrane, Q. What are the...

Q. What are the fundamental constituents of the cell membrane? The cell membrane is formed of lipids, carbohydrates and proteins. The membrane lipids are phospholipids, a sp

Factor influencing lipid oxidation - temperature, Factors affecting lipid o...

Factors affecting lipid oxidation Temperature In general, the rate of oxidation increases as the temperature is increased. Temperature also influences the relation betw

Define principle for simple staining of bacterial cultures, Define Principl...

Define Principle for Simple Staining of Bacterial Cultures? In simple staining, a single staining agent preferably a basic dye is used. Commonly used basic stains are carbol fu

Zoology, right classification of protozoa up to class

right classification of protozoa up to class

Sulphur - mineral elements, SULPHUR The major sources of sulphur are cr...

SULPHUR The major sources of sulphur are crucifers and animal proteins. Proteins present in pulses have little sulphur. Therefore, other vegetable proteins or crucifers must

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd