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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define the Microbiological Study of Water? Water is a common carrier of infectious diseases. Even clean and clear water which looks pure may be contaminated with pathogenic mic
What is NADP and NADPH? NADP is the abbreviation of the nicotinamide adenine dinucleotide phosphate cation, a hydrogen acceptor. NADPH is made when NADP binds to one hydrogen a
Biochemical Function - Essential Elements Elements Mg, Mn, K, Ca and Fe are cofactors for many enzymatic reactions. Fe is carrier of electrons in electron transfer chain. Phos
Q. What are the male pronucleus and the female pronucleus? The female pronucleus is the proper haploid nucleus of the egg cell and Male pronucleus is the haploid nucleus of the
Define Changing Body Composition and Techniques for Measuring Body Composition? Changing body composition has been suggested as a pathway to explain age-related functional decl
what are diploblastic organisms?
Insect killing jar Secure a wide-mouth glass jar with a screw top or single which closes very tightly. Place a wad of cotton in the bottom and cover it with a round piece of ca
Define Types of Root Canal Perforations According to size According to size: Small perforation: ( smaller than #20 endodontic instrument ) mechanical damage to tissue is
What is the genetic condition in which the heterozygous individual has different phenotype from the homozygous individual? This condition is called lack of dominance and it can
Define Some Caution for Inoculating Loops and Needles? 1. Heat the inoculating wire till it becomes red hot. 2. Never use hot inoculating wire for culturing otherwise cells
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