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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain Results of abdominal aneurysm surgery? Results: For ascending aorta replacement, the hospital mortality varies from 0 to 9 per cent. Aortic arch has higher risk of
Chiasmata formation takes place where crossing over occurs. Here Chromatid segments are exchanged which contributes to genetic variability. The 46 homologous chromosomes arrange at
Determine the Signs and Symptoms of Bulimia? 1. Binges, minimum twice a week for three months 2. Purging a Menstrual irregularities 3. Swollen glands Frequent fluct
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Candidiasis Candidiasis (Moniliasis) is a disease of man, animals and birds and is caused chiefly by Candida albicans. Crop mycosis (thrush) of poultry is caused by C. albicans
Q What is the alternative means for transport of substances in animals without a circulatory system? Why is blood important for larger animals? In animals that don't present th
S-Adenosyl methionine serve as a donor of methyl sets in numerous biological reactions instance for in the formation of creatine phosphate and in the synthesis of nuc
What are the soil-forming factors There are many degrees or variations of soil-forming factors, and the potential for creating different kinds of soil is enormous. However, whe
How would you prepare 90 ml of .01 M potassium dichromate dissolved in water? Molecular mass of potassium dichromate is 294 g/mol.
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