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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain the Presumptive Test - Most Probable Number Test? The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by
Q. Explain Short term complications? Short term complications may arise due to GERD which may in turn increase the frequency or severity of this disease. One of the complicatio
Leghaemoglobin - Factors Influencing Functions of Nitrogenase Leghaemoglobin is a joint product of Rhizobium and the host. It is produced during the maturation of nodule. It i
Classification of Enzymes : Enzymes are classified into the following six main categories in according with the nature of reaction the catalyze: 1. Oxidoreductases
Single Cell Proteins (SCP) The term SCP was coined by Prof. Caroll Wilson (MIT) in 1966. It means the proteins obtained from microbial sources, i.e. algae, fungi, bacteria,
#what is naturalistic theory?
Define the effect of niacin deficiency on Skin? Dermatitis is the characteristic feature of the' disease, It is symmetrical in distribution. In early stages, a bright red eryt
Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe
ISOZYMES : an enzyme having different molecular forms but catalyzing the same reaction.
Lungs - Respiration Lungs can be simple, characterised by air exchange with surrounding environment by diffusion only. These are called the diffusion lungs and are present in
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