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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Vitamin a deficiency leads to a variety of disorders of the eyes and this effects the vision. Some of the disorders due to vitamin A deficiency are given below. 1) Night Blindne
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Oseltamivir (Tamiflu) This oral neuraminidase inhibitor, started within 36 hours of symptom onset, can decrease the severity and duration of symptoms caused by either influ
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what is advantages and disadvantages of the pour plate method
Determine about the Brain tissue Brain tissue looks solid to the naked eye (it has a consistency of stiff jelly), so 'finger-grain' investigations had to await two technologic
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