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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Historical Example Population genetic theory? Population genetic theory, first developed by Sir Ronald Fisher, JBS Haldane and Sewall Wright, demonstrated how several ev
General information about disinfectants A disinfectant is a chemical that kills disease-causing agents on contact. Natural disinfection agents, such as sunlight, heat, keeping
Mention the information that the health workers get by measuring BOD of a water Body. a) Name the enzyme included in the continuous replication of DNA strand. Mention the Polar
Define the Psychological problems - Effect of Obesity? Obese people may be exposed to ridicule and discrimination in areas like employment, promotions and social interactions.
disadv of protozoa
Explain about the Weight cycling - energy balance? There are a number of obese people who keep losing and gaining weight a number of times in their lives. This is called the Yo
Describe about Abdominojugular and Hepatojugular Reflux ? The term was first coined in 1885 to test the further rise in jugular venous pressure to elicit presence of h;art fail
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All of us must have suffered from diarrhoea at least once in our lifetime. How do you feel thereafter? Well most of us must have experienced weakness, dizziness, dryness of mouth a
Normal or General Diet This diet is planned to be consistent with the Recommended Dietary Allowances (RDAs) of nutrients and is based on the food groups. It is usuall
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