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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain briefly how computed tomography (CT) helps the doctors in pinpointing the defects in the patient's body
Determine Pull-ups test for check the body strength? This test is also done to measure upper body strength or endurance by maximum number of pull-ups completed. Subject hangs f
Q. Bacteria are prokaryotic cells, i.e., they don't have a membrane-delimited nucleus. Eukaryotes have cells with a delimited nucleus. Where in these kinds of cells can DNA are fou
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