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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Genetics of Nitrogen-fixation The genetics of nitrogen-fixation is known in detail in Klebsiella pneumoniae. There are twenty genes required in organising the complete N 2 -fi
Coiling of garden pea tendrils around any support is an example of: 1. Thigmotaxis 2. Thigmonasty 3. Thigmotropism 4. Thermotaxis Thigmotropism
There are several methods by which a disease-causing organism can be transferred from the reservoir to the host organism. Direct contact : Disease - causing organism may be tr
mORPHOLOGY OF AMOEBA AND ITS ADAPTATIONS
Define Sit and Reach Flexibility component in the humans? The subject sits on a mat with legs extended straight ahead. Legs should be at right angles to a taped the r box, with
Q. Fluids requirement in ulcerative colitis? Fluids: A liberal intake of fluid should be given to prevent dehydration. The passage of at least 1200 ml of urine indicates that a
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State about visual system It is important for our visual system to adapt to recognize objects clearly in differing conditions of light. The main mechanisms concerned with
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Explain assessment of iron status - Transferrin saturation? Transferrin saturation: As iron deficiency progresses, there is a decline in transferrin saturation. With deficiency
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