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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain about the Proteins? The term protein (Greek: proleuo- to occupy first place) was first suggested by Berzelius to describe the complex organic nitrogenous substances fou
uses and advantages and disadvantages of immunolocalization
You have 200 cells and find 120 in interphase, 57 in prophase, 8 in prometaphase, and 5 in metaphase, 8 in anaphase and 2 in telophase. (None in G0 phase). it takes 18 hours to com
Explain the Presumptive Test - Most Probable Number Test? The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by
EXCRETION IN PRAWN- A pair of antennary gland or green gland and a median renal sac present. Green glands lie at the base of antennae & open out by renal aperture.
dialouge on cartoon between chlorophyll and haemoglobin
Define effect of Protein excess during pregnancy period? It is not advisable to recommend protein supplements. Reports in the literature indicate that high-protein supplements
Teaching / academic dietitians Dietitians process knowledge on all aspects 01 nutrition and dictetics. They constantly keep themselves updated in the necessary informa
Q. What do you know about Tricuspid stenosis? It is rare disease, with rheumatic etiology seen in 90 per cent of cases. In patients with rheumatic mitral stenosis only 3-5 per
How is the ovulation date estimated with the control of the woman's body temperature? One method to estimate the exact ovulation day is daily control of the body temperature ta
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