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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Illustrate about the Intra-ocular pressure It is the pressure maintained inside the eye above the atmospheric pressure. It is higher than fluid pressure in any other tissue (2-
Given four respiratory capacities (a - d) and four jumbled respiratory volumes of a normal human adult: Respiratory Respiratory capacities volumes. 1. Residual vol
Define Improvement of cereal proteins - Mutual supplementation? Improvement of cereal proteins: Cereals, in general, are limiting in lysine and threonine while legumes, milk, m
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in oogenesis only on functional egg cell is normally formed from primary oocyte. Briefly explain
Q. What is the difference between venous and arterial blood? Arterial blood is the oxygen-rich and carbon dioxide-poor blood that irrigates the tissues. Venous blood is the oxy
What can I do with this topic to make it into a brochure
What are DNA ligases? How do these enzymes participate in the recombinant DNA technology? DNA ligases are enzymes specialized in tying the complementary DNA chains that produce
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