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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Brucellosis Brucellosis among cattle, buffaloes, sheep, goats, pigs produces heavy economic losses due to abortions in late pregnancy, infertility and reduction in productivit
Bond specificity Some enzymes act on a particular bond (glycosidic, peptide, ester etc.). Examples: pepsin, trypsin, chymotrypsin etc. are all only acting on
Define protein requirements of Preschoolers? For Preschoolers the protein requirements of children of various age groups are computed by the factorial method as employed by FAO
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Assume that you have 1 mL of a solution amylase (an enzyme) at a concentration of 15 mg protein/mL. Calculate the volume of diluting buffer that you would have to add to 1.0 mL of
The various types and their description of Heart failure are as follows: Left Sided Versus Right Sided Heart Failure Predominantly left sided failure is seen in left ventr
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Q. What is the main molecule responsible for the absorption of photic energy for photosynthesis? And where is that molecule located in photosynthetic cells? The chlorophyll mol
explain satellite chromosomes
Define interaction of vitamin k with Calcium? Since vitamin D functions have an impact on calcium metabolism, an inter-relationship exists between vitamin IS-dependent proteins
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