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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Physiological and Socio Psychological Factors - public nutrition? Food related behaviour depends on a combination of biochemical factors, mainly, physiological aspects a
Circulatory Assist Devices : Intra aortic balloon pump (IAUP) was introduced by Kantrowitz (19hX). It is also known as Counter pulsation or Diastolic augmentation.
Define Requirements for underwater weighing method? • The equipment required to perform hydrostatic measurements is bulky and maintenance intense. • A large tank of water, usu
STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERMS
What are the main functions of (a) the basal (Malpighian) layer, (b) the cornified layer of the skin? (a) The basal (Malpighian) layer makes new skin cells and the pigment
Define about the Glutathione peroxidases - Selenium? The role of selenium in the cytosolic enzyme, glutathione peroxidase (GSHPx), was first illustrated in 1973. Four selenium
Malarial disease Malaria is a disease known since ancient times almost from 5th century B.C. The word malaria actually means bad air. It was believed that malaria is c
How to reduce the stresses on the implant i) There is need to reduce the stresses on the implant - ii) Frequent recall for maintenance or hygiene appointments. iii) Gingi
Define respiration in animals
Social Determinants of Health - Unemployment That unemployment gives rise to social insecurity and psychological stress is well recognised. Fears and uncertainties are known t
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