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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Descending type of paper chromatography? In descending chromatography, the movement or flow of mobile phase is in downward direction. In this technique the solvent is ke
The physician orders 2 liters of lactated ringers over 16 hours. The drip factor of the tubing is 20 gtt/cc. What will be the flow rate? a. 33.33 or 34 gtt/min b. 41.66 or 42
Mollusks
Characteristic features of Phylum Cnidaria All are aquatic animals. Radial or biradial symmetry around an oro-aboral axis, but no head. Diploblastic, with an epidermi
At the end of the ventricular contraction, maximum arterial pressure is attained and blood flow is most rapid. This is correctly termed the: a) Arterial maximum b) Diastolic
Coelenterata – General characters and classification.
Diploidy and Haploidy :: In the chromosomal complement given species not all the chromosomes are different from each other .In fact these are in pairs ,i.e. every two chrom
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Treatment options for ailing and failing implants are varied. The clinician should start conservatively and progress to more aggressive therapy. The overall goal of therapy is
charactoristics of offset
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