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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Theory of Germplasm (i) 'August Weismann' (1889) suggested the theory of continuity of germplasm. (ii) He referred to the reproductive cells as germplasm and
describe each and every step in nutrition in animals?
Meaning of Statistical Investigation: An investigation means a systematic search for the knowledge or the truth. In statistical investigation the search for the knowledge is do
Explain Transfer of Culture from One Liquid Media to another Liquid Media? 1. Inoculating loop is to be held in the right hand. It is sterilized by incineration in the inner bl
Q. Explain Consciousness as inner state? Consciousness as inner state: We are conscious of thoughts, images, emotions and memories within ourselves though they may not have phy
what is the bet way to asses energy production in the body of an athlete?
Pre Pregnancy Counselling To prevent excess spontaneous abortions and congenital malformations in infants of diabetic mothers, diabetic care, education and counselling must be
What isthe Riccia
Immobilisation of cells Immobilisation of cells enables Re-use of biomass by rotation of cells Separation of cells from the medium and Leaching of metab
Define Transverse section of body of male Ascaris? 1. Body wall consists of the following: (a) Cuticle is the outermost layer. It is a thick, tough, elastic membrane
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