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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is signified by saying that in relation to a given trait conditioned by a gene with two different alleles the gametes are always "pure"? To say so that the gametes are pur
Explain the Membrane Equilibria? A semipermeable membrane used to separate two liquid phases can, in principle, be permeable to certain species and impermeable to others. A mem
SCOPE OF BIOLOGY- Present century is witnessing explosion of information in modem biology directly related to life and health. Issues like global warming, pollution, populat
Determine the reliability of the battery The issue of reliability of the battery has been addressed, with reasonably successful results. Clinical interpretation of the battery
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how efect of gravity on roots?
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what are the general characteristicss of porifera
The oxidation of glucose produces NADH that is oxidized by the electron transport chain. What compound is the terminal electron acceptor of the electrons carried by NADH? -O2
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