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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Head stream of Highland brook zone This is formed by a number of small streams, which originate from a marsh, a spring or a glacier. This is a small shallow zone with an irreg
what is the function of nucleus
Place a slide of Amoeba (common species is A. proteus) under tlie lnicroscope and focus it under low power (5x x 5x), and observe it carefully. Amoeba looks like an irregular colou
Discuss about the Common migraine This is the most frequent type, occurring in more than 80% of migraine sufferers. There is no clear aura as there is in classic migraine, but
Why Fats have highest heat energy density? Fats have highest heat energy density of 9 Kcal/g. It is the major storage form of energy in body requiring least space and minimum w
electron transport chain
Q. What are the symptoms of congestive cardiac failure? Congestive cardiac failure is a progressive form of cardiomyopathy. The most classical symptom is fluid imbalance due to
Energy Metabolism In the preceding sections of the unit, you studied how the products of digestion of food, viz: amino acids, sugars and fatty acids are absorbed and transport
Explain about the Spectrophotometer? The spectrophotometer, a key instrument today in biomedical laboratories, was invented in 1939 by the American chemist Arnold O. Beckman (1
biological significance of biology
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