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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
In native-valve IE, acute CHF occurs more frequently in aortic-valve infections (29 per cent) than with mitral (20 per cent) or tricuspid disease (8 per cent). CHF may develop acut
Cell secretion is the elimination to the exterior of substances formed by the cell (for example, hormones, mucous, sweat, etc.)
Q. Silent Myocardial Ischaemia? Ans. This deals primarily with those who have never had symptoms recognized as being of cardiac origin. Others, who have had recognized myo
Explain the Sensory Respiratory center - Feature of Medulla Respiratory center: The center for regulating rate and depth of respiration is present in the medulla. Here, nerve i
OFF PUMP SURGERY : In spite of great advancements in techniques of cardio pulmonary bypass, it is still not physiological. There can be various complications related to perfusion
Please help with the following: 32P is an unstable isotope of phosphorus that has a half-life of 14.29 days. Atoms of this isotope undergo beta decay in which a neutron decays i
Vasa are a maternal-effect gene whose product functions in development of the posterior end of the Drosophila embryo. runt is a non-maternal-effect gene whose product functions in
Studying life histories of insects Cut large rectangular holes in the sides of a big cardboard container and cover them with muslin folded over at the edges and gummed or paste
Accident Proneness The close observation of accident causation theory brings out this fact that most accidents can be traced to error on the part of a number of individual em
Describe Pressure Controlled Ventilation (PCV) This is a pressure cycled mode (machine delivers a set pressure - the tidal volume generated depends on how compliant the lungs
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