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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
In f e c tiou s Diseases Infectious diseases inflicts major economic losses as the disease spreads from one animal to other and large number of animals are affected with t
What are the cells responsible for the production of antibodies? The cells that make antibodies, i.e., the cells of the humoral immune system, are the B lymphocytes (B cells).
Depth and Currents Depth The sea is very deep varying in different regions. Generally life extends to all depths but is confined more to the continental shelf and
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Q. What are the protective structures of the central nervous system present in vertebrates? In vertebrates the brain and the spinal cord are protected by membranes, the meninge
What is digestion? The ingested food material is broken down into smaller constituents which are assimilable by the blood. The process through which the major nutr
Photosynthesis Photosynthesis - the process by which plants utilise energy from sunlight to convert carbon dioxide and water for the synthesis of sugar. This sugar can then be
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The protein collagen has a high proportion (20% or more) of A. asparagine and glutamine B. proline and hydroxyproline C. glycine D. both A and B E. both A and C F
Multiple Fission - Types of Asexual Reproduction Multiple fission is a variation of fission where the parent divides mitotically into a number of smaller units that are the da
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