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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is the meaning of Chelicifore? These feeding mouthparts are found on most, pycnogonads. Though the proboscis is the main feeding structure in these animals when chelicifor
what are the function of nucleus
How the needles are classified by thier point geometry Needles may also be classified by their point geometry; examples include: - taper (needle body is round and tapers smo
Q. What are the Causes of constipation? The most common causes of constipation are poor elimination habits, a lack of fibre in the diet, insufficient fluid intake, lack of exer
How Sugar is used in Bakery Application? In bakery applications, sugars are used to impart flavor, aroma and color. During the mixing process, excess gluten development can mak
What should be the minimal distance between the implants or an implant and a natural tooth. The minimal distance between the implant and the adjacent tooth should be 1.5 -2 mm
Do moulds need water for growth? Place a spoonful of dry cereal like as rice or oatmeal in a sterile culture dish. Place a like amount of the similar cereal cooked in another c
Linkage of Health With Development Health, in its comprehensive or holistic sense, refers to the state of completephysical, mental, social and spiritual well being of a person.
What is homogenization Emulsions may also be stabilized by a process known as homogenization, in which the size of the dispersed fat globules is greatly reduced to more or less
Conformational Protection - Azotobacter In conformational protection a Fe-S redox protein provides protection to the enzyme. The protein gets oxidised in the presence of oxyge
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