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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Slight or no Metamorphosis In primitive wingless insects (Apterygota) such as spring tails, silver fish etc. and in secondarily apterous insects the young ones which hatch fr
A d v a nce d vaccine technology: Genetic engineering technology is the major scientific revolution of 20th Century. This has helped in developing vaccine
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Septa prevent oxygenated and deoxygenated blood. Give reason
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