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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
(a) Give two examples of diseases which can be spread by contaminated drinking water. (b) How can the spread of such diseases be prevented? (a) Typhoid and cho
Q. How does the hypophysis- corpus luteum negative feedback work? What is the name given to the atrophied corpus luteum after this feedback process? After ovulation the estroge
EMBO L Y - The embolic morphogenetic movements are concerned with the inward migration of prospective endodermal and mesodermal blastomeres from the external surface of blast
Classification of Enzymes : Enzymes are classified into the following six main categories in according with the nature of reaction the catalyze: 1. Oxidoreductases
Impact of changes in diet in the management of diabetes mellitus Changes in diet are an essential component of comprehensive diabetes care and treatment. In this section you w
Explain about the Neuro Trauma? Neuro or head trauma includes brain injury, skull fractures, extraparenchymal or internal brain haemorrhage, Brain injury can be divided into th
Insulin binding to insulin receptors in the plasma membrane of a A. liver cell will lead to an increase in the intracellular amounts of cAMP in the liver cell. B. beta-islet
what so special for phylum annelida
Characters of vitamins: Vitamins are organic substances. They are micronutrients and are required in small quantities. They are not synthesised in the body and therefo
Sheep and goats The disease is readily transmissible to human being from goats, and is known as Malta fever, Mediterranean fever or undulant fever. The chief causal agent is B
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