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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
A container is filled to a depth of 20.0 cm with water. On top of the water floats a 30.0-cm-thick layer of oil with specific gravity 0.700. What is the absolute pressure at the bo
Q. show the Symptoms of constipation? Have you ever suffered from constipation? Do you recall the symptoms associated with the problem? Yes, the symptoms were specific to havin
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URETERS From hilum of each kidney emerges out a tube. Wall is thick. Lumen is star shaped. 28 cm. long. Transitional epithelium present in wall. Open in to urinary bla
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