Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Explain the digestibility coefficient - proteins, Explain the Digestibility...

Explain the Digestibility Coefficient? You have earlier learnt that dietary proteins are hydrolyzed to amino acids during digestion. The digestion begins in the stomach by the

Bomb calorimeter, Normal 0 false false false EN-IN X-...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain working of dietitians and nutritionists, Dietitians and nutritionis...

Dietitians and nutritionists Dietitians and nutritionists, who are associated with hospitals and clinics generally have regular work hours. At times, they may be required to wo

Endocrine glands that regulate sexual activity in males, Q. What are the en...

Q. What are the endocrine glands that regulate sexual activity in males? How does this regulation work and what are the involved hormones? In males the sexual activity is regul

Define ion exchange chromatography, Define Ion exchange chromatography? ...

Define Ion exchange chromatography? In this method a reversible exchange of ions is effected. Usually resins and ionic solutes are the two components involved in ion-exchange.

Hiv, The chances of transmission in female is mor than males

The chances of transmission in female is mor than males

Is rabies caused by virus or by bacteria?, Q. Is rabies caused by virus or ...

Q. Is rabies caused by virus or by bacteria? How is it transmitted? Which organs and tissues are affected by the disease? How is it prevented and treated? The Rabies, as well k

radioactive pollution, Radioactive pollution is a very special form of pol...

Radioactive pollution is a very special form of pollution of air, water and soil with radioactive material. Its nature of contamination and effects are different from those of othe

What volume of 1.20 m naoh solution must we add, You have 875 mL of a 0.37 ...

You have 875 mL of a 0.37 M acetic acid solution. What volume (V) of 1.20 M NaOH solution must you add in order to prepare an acetate buffer of pH = 4.36? (The pKa of acetic acid i

Which are the germ layers present in cnidarians, Which are the germ layers ...

Which are the germ layers present in cnidarians? Which tissues of the animal do they originate? These beings there ectoderm and endoderm, two germ layers. Animals with only two

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd