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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Deoxyribonucleic Acid (DNA) - polymer of nucleotides containing genetic information that codes for proteins Nucleotide - a monomer of DNA consisting of a ribose/deoxyribose sug
Using the words 'cilia' and 'mucus', describe, very briefly, how the body gets rid of dust which enters the lungs. The lining of the air passages makes mucus which traps dust p
How sugar is used in Puddings, Sauces and Pie Fillings? When dry starch is added directly to a hot liquid, the particles on the outside tend to cook first, lumping the raw star
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Q. Which kind of chemical bond maintains the pairing of each chain in the DNA molecule? To form the DNA molecule, purine bases bind to pyrimidine bases by intermolecular bonds
Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made
Another important genus of mycotoxin producers is Fusarium, many species of which produce members of the trichothecene family of mold metabolites like deoxynivalenol, neosolaniol a
Dengue Dengue fever is a viral disease transmitted by mosquito bites that occurs worldwide in tropical and subtropical areas. Major epidemics have occurred in recent years in
What are the types of brain?
Sarcodine protozoans are amoebas, and there are two types of them: shelled and naked. As you examine the prepared slides you should be able to see water expulsion vesicle, nucleus,
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