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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Complete feed system in ruminants Complete feed system for feeding dairy animals is a departure from conventional individual rationing method. It involves the self-feeding of
What are zymogens? Zymogens, or proenzymes, are enzymes secreted in inactive form. Under some conditions a zymogen shifts to the active form of the enzyme. Zymogen secretions i
Debate over ethics of using gene therapy for treating human beings started since the discovery of recombinant DNA technology. The mere fact that it involves the alteration of DNA w
why obelia is of special interest in zoology
What is lyophobic If the affinity of the dispersed phase to go into or to remain in colloidal dispersion is slight, the dispersed phase is said to be lyophobic (solvent repelli
How sugar used in Flavors and Mouthfeel? In frozen desserts, sugars balance flavor and mouthfeel. Since low temperatures tend to numb the taste buds, sugars enhance flavors, th
Q. What are the sources of dietary fibre in our diet? The sources of dietary fibre include whole grain cereals, legumes, whole pulses, leafy vegetables, vegetables like peas,
What is the structural representation of a carboxyl group? Carboxyl groups have a carbon attached to single hydroxyl group by a simple bond and to one oxygen by a double bond.
A solid compound was found to contain 42.11% C, 51.46%O and 6.43% H by weight. The molecular weight of the compound was 341. What is the molecular formula of the compound?
Contamination of the Implant Body before Insertion The implant may be contaminated by manufacturing error, by the operator, from non titanium instrumentation and by the bacteri
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