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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. Which type of cell came first in evolution - the eukaryotic cell or the prokaryotic cell? This is an interesting problem of biological evolution. The majorly accepted hypoth
How are the fruits formed? The fecundation is in angiosperms triggers the release of hormones that act upon the ovaries. The ovary wall after that it develops into a fruit that
Explain Differential Media - Culture Media? It allows differentiation among morphologically and biochemically related group of organisms. It contains certain ingredients which
ROLE OF FAT OR LIPIDS - Made up of fatty acids & glycerol. Linked by ester bond. Maximum quantity of energy is librated. Helpful in temperature regulation. As stored f
What is a test tube and how is it used? A test tube is a clear, cylindrical glass tube generally open at one end and rounded at the other, used in laboratory experimentation.
Digestive Enzymes Now let us consider the general principles of digestion that are applicable t6all animals. We will start with the digestive enzymes that breakdown the large
Explain Photolithoautotrophs - Nutritional Types of Microorganisms? These microorganisms use light as an energy source, inorganic substance as electron source and CO 2 as the
Vitamin A (Retinol) Purified vitamin A is a viscous yellowish oil. It is freely soluble in most organic solvents such as methanol, ether, acetone, chloroform and petroleum ethe
If a person has a stomach ulcer (that is aggravated by excessive hydrochloric acid {HCl} secretion ), why on earth would he or she be drinking milk to feel better, when milk is als
genetic reason.medications
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