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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are
Ur o l it h i a s i s The presence of calculi (urolith) in the urinary system is termed as urolithiasis, and the disease is characterized by distension of bladder and
A plant grown from one of Mendel's yellow peas is selfed. Five progeny peas are obtained from this self and they are all yellow. If the original selfed plant had been homozygous, w
GIVE THE SHORT DETAIL OF HE BIOLOGICAL METHODS
Q. What are the functions of the osseous tissue? The main functions of the osseous tissue that are to provide structural rigidity to the body and to delineate the spatial posit
Q. What are the fundamental constituents of the cell membrane? The cell membrane is formed of lipids, carbohydrates and proteins. The membrane lipids are phospholipids, a sp
Factors affecting lipid oxidation Temperature In general, the rate of oxidation increases as the temperature is increased. Temperature also influences the relation betw
Define Principle for Simple Staining of Bacterial Cultures? In simple staining, a single staining agent preferably a basic dye is used. Commonly used basic stains are carbol fu
right classification of protozoa up to class
SULPHUR The major sources of sulphur are crucifers and animal proteins. Proteins present in pulses have little sulphur. Therefore, other vegetable proteins or crucifers must
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