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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain Hypertensive Disorders of Pregnancy? Hypertension may have been existing before pregnancy. Alternatively, a mother may develop hypertension during pregnancy, a pregnanc
Describe the Intensity of S 2 in second heart ? Loud A 2 occurs in patients with hypertension, when aorta is closer to anterior chest wall owing to root dilatation or TGA or
Low Ca ++ in the body fluid may be the cause of: 1. Tetany 2. Anaemia 3. Angina pectoris 4. Gout Tetany cause low ca ++
Explain Nutrition diagnosis Nutrition diagnosis is the identification and labelling that describes an actual occurrence, risk of, or potential for developing a nutrition probl
A haploid cross is made in which the mutant allele has GC at base position 200 in the DNA of a certain gene, and the wild type has AT. What proportion of progeny will have the hybr
In a numeric pyramid is it possible for the base to be smaller than the other levels? Ever since the numeric pyramid represents the quantity of individuals in each trophic leve
SIMPL E PROTEINS The proteins are made of amino acids only. Additional chemicals are absent. These are of two types - (i) Fibrous (ii) Globular ( i )
In a neuron with a resting potential of -65 mV, the distribution of which ion across the neuronal membrane represents the greatest potential electromotive force (EMF)? A. Potassiu
E s o pha g e a l obstruction It is also known as choke. It may be acute or chronic and is characterized by inability to swallow, regurgitation and bloat. E t
ANIMALS THAT ARE PROTOZOA????
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