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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain about the Secondary Protein Derivatives? These are the products of further hydrolytic cleavage of the protein molecule, Examples include: a) Proteoses: Soluble in wa
List the advantages and disadvantages of stage 2 surgery using a tissue punch. Advantages - Less traumatic to surrounding tissue - Faster progress to the impression proce
While working in the lab, you isolate a cell from a mouse and a cell from a human and fuse them together to form a live, hybrid cell. You then use an antibody that recognizes all m
RESPIR A TIO N IN INSECT (COCKROACH) - Respiration is direct. So metabolic rate is high. This system is related to each cell of the body so respiratory pigment is ab
Define Significance and Impact of Plants and Animals on Human Life? Plants and animals both have a great significance and unparalleled impact on human life. Most of our needs a
What is yersiniosis? Give its symptoms. Yersiniosis caused by the bacteria Yersinia enterolytica. • Causes severe abdominal pain, fever and diarrhoea.
What is the karyotype found in Down syndrome? Down syndrome is the aneuploidy that is a numeric alteration of chromosomes within the cells compared to the normal number of chro
STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERMS
Explain the OBJECTIVE of Cardiac Rehabilitation ? After going through this unit, you should be able to: 1 identifies the eligible candidate for cardiac rehabilitation and be
Q. What is Fenugreek seeds? Fenugreek seeds: 'Commonly known as methi seeds in Hindi. They are commonly used in Indian cuisine in chutney and even in pickles and several dishes
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