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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
cellis basic unit of life
Explain Generation of sound in MVP? Generation of sound in MVP: These are generated due to sudden tensing of redundant leaflets and elongated of chordae tendinae. Interventions
Lungs - Respiration Lungs can be simple, characterised by air exchange with surrounding environment by diffusion only. These are called the diffusion lungs and are present in
does jellyfish depend on solarenergy
what are the two reactants and the two products of a dehydration reaction/ What are the two reactants and products of a hydrolysis reaction
Mosaic Endosperm - Variants of Endosperm In some plants patches of two different colors appear in the tissues of the endosperm providing a mosaic design. In maize, red and whi
T ypes of hormones - (i) Releasing and Inhibitory hormones - Hormones of hypothalamus and kidney (renin) which control secretion of trophic hormones. (ii) Trophic hormon
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explain hadzi''s theory with reference to metazoa
Determine How Nutrition used to Prevent Dehydration? Maintaining optimal hydration status along with sufficient muscle glycogen stores delays the onset of fatigue and enables
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