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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Contagious ecthyma The contagious ecthyma virus is immunologically distinct from vaccinia, but similar to the causative agent of pseudo-cow-pox. It belongs to the genus Parapoxvi
various parameters which are used in water quality measurement
A biologist examines a series of cells and counts 140 cells in interphase, 10 cells in metaphase, 4 cells in anaphase and 7 cells in telophase. if complete cell cycle requires 24 h
Results: Early mortality for tricuspid valve replacement is around six per cent. But depending on the patient's class of symptoms and the number of concomitant procedures, this c
WHAT IS THE TYPICAL REPRODUCTION CYCLE OF A DNA VIRUS
Define the Roller Drying (Drum drying) Method? One of the important techniques for drying liquid food products is the roller drier. Like spray drying, roller drying can only be
What is an ecosystem? An ecosystem is the system that composed of biotic and abiotic factors in interaction.
What are typical structures of the seed? What is the endosperm? A typical seed is the composed of the embryo, endosperm and shell. Inside seeds of angiosperms there are one or
Gibberellins - Plant Growth Substances In late 1920's Kurosawa observed that the medium in which the fungus Gibberella had grown could cause the abnormal tallness in rice seed
Q. How is the finding of ribosomes inside mitochondria and chloroplasts explained? It is a strong hypothesis that chloroplasts and mitochondria were prokaryotes that associated
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