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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Determine the term - Leuconoid sponge. The most complex of the three different sponge architectures. Choanocytes are found in chambers, and there is not any spongocoel. Water e
Plot the data as a suitable graph. Join the points with the best-fitting smooth curve.Temperature oC(5,10,11,13,17,19) Time taken for eggs to hatch/h 230 90 70 30 25
Poultry Mycoplasma gallisepticum causes chronic respiratory disease (CRD) in chickens. The incubation period is 1-3 weeks. In young birds, the disease is less severe than adult
What is the positive atom referred to in ionic bonds and write a monovalent and divalent example.
Q. Explain causes of Shigellosis? Shigellosis is caused by the genus Shigella and is a common enteric (intestinal) infection. The genus has four serological groups,
Atopic individuals are those who are having abnormal high levels of circulating IgE and more than normal number of oesinophils.
How to increase in the amount of glucokinase in liver Consumption of a meal rich in carbohydrate or administration of insulin initiate an increase in the amount of glucokin
Q. Is all the cartilage in the body the same? There are 3 types of cartilage in the human body: 1) hyaline cartilage; 2) elastic cartilage and 3) fibrocartilage. Hyaline cartil
Q. Concerning their size and basic morphology how and why do the male and the female gametes differentiate from each other? The female gametes are big cells full of vitellus (n
Define Role of Glucose-6-phosphate dehydrogenase - Gene Expression? Glucose-6-phosphate dehydrogenase (G6PD) is another enzyme involved in lipogenesis that is regulated by diet
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