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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Precautions for estimation of vitamin c in a solution? 1. Rinse all glassware with 3% metaphosphoric acid before you begin your practical and subsequently each time you
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The question asks . . . 18 moles of moles of maltose are completely oxidized by the reactions of the final oxidative pathways (Embden-Myerhoff Pathway and Krebs Cycle). How many AT
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Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures. Face-centered cubic (fcc) struct
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Define Factors affecting Absorption of Dietary Iron? There are mainly four factors that determine iron bioavailability absorption from the diet. These include: i) Form of ir
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