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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
How different are fecundation in osteichthyes and in chondrichthyes? In chondrichthyes fecundation is internal by means of copulation. In osteichthyes fecundation generally is
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Determine the intracellular concentration of TRVA Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectly c
ANIMALS THAT ARE PROTOZOA????
Inter specific Incompatibility This type of incompatibility is characterized by the prevention of fusion of gametes between members of different species. The incompatibility
Q. How does the pulmonary ventilation affect the carbon dioxide concentration in blood? What happens to the carbon dioxide concentration and to the blood pH when the respiratory fr
Explain Identifying features of Rhizopus? Macroscopically Rhizopus appears as a white cottony mass (look at rhizopus growth on media above) growing rapidly and spread over enti
Explain Micronutrient deficiencies during kwashiorkor? Almost all the children manifest anaemia (due to iron deficiency) of some degree. Eye signs of vitamin A' deficiency are
Suppose that a portion of double-stranded DNA in the middle of a large gene is being transcribed by an RNA polymerase. As the polymerase moves through the sequence of six bases sho
Apical Ectodermal Ridge (AER) We have described earlier that the AER persists at the tip until the last phalangeal cartilage begins differentiation. The following experiments
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