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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Adult Botulism The symptoms of botulism may develop between 12 and 72 hours after the ingestion of toxin containing foods. Symptoms include nausea, vomiting, fatigue, dizzine
An ecosystem consists of two types of components: (a) Abiotic components: This includes the non-living components of the ecosystem. These are physical and chemical fact
What is the difference between xylem and phloem? Both xylem and phloem are vascular tissues found in a plant. Xylem is a tubular structure, which is liable for water transport
Define the Process of Digestion of Proteins? The daily protein intake (of about 50-100 g) and the protein of enzymes, sloughed (bled or drop off) epithelial cells and mucins, w
classification on protozoans
Ecosystem Control Another important aspect of ecosystem functioning that is how it maintains its ecological balance. By now, it must be obvious to you that an ecosystem is a d
What is the role of Linked Traits in genetics? In his study of inheritance in the fruit fly, Morgan observed that certain genes are distributed together, or linked, rather than
How does Respiration in animals
Describe Class Ophiuroidea in general way? Brittle stars have a central disc with five long, thin, flexible arms that can be detached from the body at will in times of danger.
Explain the Dietary Management in Trauma? Trauma As a result of metabolic responses to injury, there is an increase in the energy expenditure. Oxidation of body fat stores take
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