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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Nutritional Requirements of Fats and Oils? As with the other macronutrients, we do compute requirements of visible fats too for all age groups and give guidelines for se
The diet of a MSUD patient (child) should therefore involve: • Measured quantities of natural protein or leucine from foods. • A BCAA free protein, vitamin and mineral supp
Explain the term Lipids? If somebody discovers that you have some knowledge of nutrition, you will be promptly shooted with two questions, first, "Oh! You really can tell how m
Since erythrocytes red blood cells do not hold any sub cellular organelles they are essentially a membranous sac for carrying haemoglobin their plasma membrane is a convenien
Q. ST Depression only on Recovery? Although ST depression during exercise often persists into recovery, it may not manifest until exercise has been terminated. The reasons for
In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t
I need to come up with a thesis topic for a 8-12 paper. It can be on anything related to Human Biology and I am struggling to come up with a topic/thesis statement. Any help will b
Determine about the term - heterogeneous mixture The solid phase made up of soil and other source material for nutrients, is a heterogeneous mixture. Each nutrient source contr
Prevention and control The organism is sensitive to penicillin, tetracycline and other broad-spectrum antibiotics. Newer generation drugs are being used for the treatment of affec
Isolation of dna fragments bound strongly with protein 1. Through pcr Pcr permits isolation of dna fragments from genomic dna through selective amplification of a spe
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