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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Transport with the Help of Membrane Protein Even though, the lipid bilayers do not permit the entry of polar molecules such as ions, sugars, amino acids, nucleotides and cell
History of evolution? Evolution is usually defined as "change over time." In spite of the incredible diversity of life found on Earth, many fundamental characteristics are shar
What are the hormones secreted by the neurohypophysis? What are their respective functions? The neurohypophysis secretes oxytocin and the antidiuretic hormone (ADH). Oxytoci
Chromosomal Type-Genetic basis of Sex Determination It refers to the condition where the genes involvcd in sex dcterrnincltion arc located on specific chromosomes known as the se
Define Sample Titration for Saponification Number of Fats? Measure 2 ml of oil with the help of a pipette and transfer this oil to a 250 ml conical flask. Add 40 ml of alcoholi
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Define the term - Cholera Cholera is caused by the gram negative, V. cholerae, which is acquired by ingesting food or water contaminated by faecal material from patients or
#question.what do u mean by parasitic habit.give account of various parasitic adoptation in animal.
What is Saliva Saliva is the colourless viscous fluid. It helps in swallowing the food by lubricating the food and the neutral pH of saliva prevents the decalcifica
Q. Show Coding for stimulus intensity? Coding for stimulus intensity: If AP's are all-or-none responses that do not vary in amplitude, how does the NS distinguish between stim
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