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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Precautions for Quantitative Determination of Viable Microbes? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Asep
Characteristics of Soil Profile The profile characteristics studied in the field consist of locating the soil hor izons (based on its colour description that includes the col
Explain Water as a lubricant? All fluids have lubricating as they can make it easier for the solid materials to slip over one another. Water-based fluids act as lubricants in
Q. What are the Pseudo-yeasts? These are like true yeasts but do not form spores. All the members of this group are particularly unsuitable for fermentation purposes as they p
Q. Measurement and Evaluation of Food Texture? Kinesthetic characteristics deal with the sense of feel, just like the characteristics of appearance deal with the sense of sight
Q. Vitamins and minerals requirement in diabetes? The recommendation of vitamins and minerals is the same as for general population. However, lower levels of magnesium are ass
Define Dietary Goals in Parkinson's Disease? The main goals include: - Maintain desirable weight - Promote absorption of anti- Parkinson's drug levodopa - Lessen swall
Q Show Geniculate Bodies of the thalamus? THALAMUS - two large circular shaped masses at "roof" of 3rd brain ventricle. Contains many different nuclei which function as relay
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Define Regulation of Water Balance? The input of water, as well as, its loss can be highly variable due to individual habits and environmental factors; in spite of this, the to
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