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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is mucoperiosteal flap Silk is easy to handle, ties with a slip knot, and is relatively inexpensive compared with other nonabsorbable suture materials currently available.
Arrange the following processes sequentially to define the translocation of food through sieve tubes. i. Unloading of sugar in sink cells (or cells of root). ii. Uptak
Under which conditions do aerobic cells carry out fermentation? Some cells that as a rule get energy from aerobic cellular respiration can carry out fermentation when oxygen is
Protein Digestion Enzymes that digest proteins are divided into two groups endopeptidases and exopeptidases according to site of their action in the protein molecule. Endopept
Results : In critical pulmonary stenosis in infancy, surgical mortality is 6 per cent. For children and adults with isolated pulmonary valve stenosis, the mortality approaches 0
Ask questionLIMITATION OF ECOLOGICAL PYRAMIDS #Minimum 100 words accepted#
Blood functions to maintain homeostasis in the human body through all but which of the following: Answer moving carbon dioxide away from cells following completion of aerobic metab
write six characteristics of bacteria
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Contrast sense Sense of contrast is the ability to perceive slight changes in luminance between regions which are not separated by definite borders. This is as important as to
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