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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define Assessment of iron status - Haemoglobin and Haematocrit? In the final stages of iron deficiency, anaemia occurs. Haemoglobin and Haematocrit levels indicate prevalence o
An adequate level of pressure is needed in the arteries to keep the blood flowing through the cardiovascular system. The chief determinant of arterial pressure is the volume of blo
what is the structure of hiv virus?
Intracellular Digestion We all know that unicellular organisms do not have a separate alimentary canal system. All the functions of life are carried out inside a single cell.
A person is declared to be dead upon the irreversible cessation of spontaneous body functions; brain activity, or blood circulation and respiration. However, only about 1% of a per
What is monera? One of the 5 main kingdoms contains bacteria and blue/green algae. Does NOT have a cell membrane, or in other words, is made of prokaryotic cells. Actually,
Two major objectives must be achieved to treat IE effectively. The infecting micro-organism in the vegetation must be eradicated. Also, invasive, destructive intracardiac and foca
What is the typical localization of the tropical forests regarding latitude? Tropical rain forests, as the Amazon forest and the Congo forest, are typically located in low lati
Let us learn about the factors that affect metabolism: 1) More energy is required when muscular work is carried out. 2) The larger the surface area of the body, more heat is
Q. What do you know Zoological Parks? Zoological park has two faced aspects, the relationship between man and beast. There is awe and there is slaughter. There is worship and t
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