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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Radiobiology : This is the study of effects of radiations on living organisms. Radiobiology is a branch of science which concerned with the action of ions. Radiobiology is the radi
Q. What are the major events of the final mitotic period? The final mitotic phase is the telophase. In the telophase the following events occur decondensation of chromosomes ea
1. Using specific examples describe how variations in DNA sequence between individuals can lead to risk of disease. Describe how a range of techniques have been adapted to detect s
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It is important to recognise underlying causes and precipitating factors of heart failure for its appropriate management. That would also help in prevention and treatment of heart
Milkers’ nodules Milkers’ nodules are caused either by cowpox virus, an orthopoxvirus or pseudocowpox virus, a parapoxvirus. These are relatively benign lesions that occur most co
The sequence for the Beta Actin gene (ACTB) is given below. Which pair of primers (designed to amplify the entire sequence below) could be used to successfully produce the beta act
Tests of shelled amoebas are made from a variety of substances including sand grains, chitin and substrate, calcium salts, and even silica. These shells still accumulate in ocean d
lichens
distinguish between striated and cardic muscles
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