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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. How is the gymnosperm seeds formed? What are the ploidies of the structures that compose the seeds? Their seeds are produced from differentiation of the megasporangia in the
Primary effects 1. Nuclear contamination 2. Biological contamination 3. Chemical contamination 4. Destruction of infrastructure facilities. 5.
What is NAD? What is the role of the NAD molecule in glycolysis? NAD (nicotinamide adenine dinucleotide) is a hydrogen acceptor essential as reductant (to receive hydrogen) in
Figure shows the flow of energy through the trees in a forest ecosystem.The numbers represent inputs and outputs of energy in kilojoules per m 2 per year. (a) (i) On Figure,
Adverse Effects of Famciclovir Famciclovir has been generally well tolerated. Headache, nausea and diarrhea have been reported. Like acyclovir, famciclovir has been associated
In Dental Traumatology Traumatic injuries in young kids (8 to 14 years old male > female) are commonly nowadays. Dental traumatic injuries are mostly in anterior teeth
pls explan 2 puc lesson
Q. What is the estimated percentage (in mass) of water in the human body? Is this percentage expected to be larger in the adult or in the old individual? Approximately 65% of t
Define Reaction of Fehling Soxhlet method? The reducing sugars formed are acted upon by the alkali of Fehling's solution to form enediols. These enediols are very unstable and
Q. Zonation in the ocean? The oceans play a major role in determining the climate and sustaining life on earth. Oceans help to redistribute the solar energy, through ocean curr
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