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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. How is digestion performed in protozoans? Digestion in protozoans is intracellular digestion organic material degraded inside the cell and is internalized. Protozoans get
These are safe and free of systemic side effects. However, gastrointestinal side effects are common, and compliance is poor. The average LDL decreases by approximately 15 per cent
Explain about the Observation and Classification of Arthropoda? Introduction In the previous unit you have examined the representatives of artliropoda. In this unit, you wi
What is the valve that separates? What is its function? The valve that divides the stomach from the duodenum is the pylorus. It has the function of keeping the food bolus within
The nominalists deny the existence of Plato's "types". For them only individuals exist. Species are man-made artificial abstractions. But naturalists, the biologists who have actua
Q. What is Gastritis? Gastritis is an inflammatory lesion of the gastric mucosa, (the inner lining of the stomach). The problem is seen in two forms: a) Acute gastritis
ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.
Some hyperthermophilic organisms that grow in highly acidic (pH2) habitats belong to the two groups: 1. Eubacteria and archaea 2. Cyanobacteria and diatoms 3. Protists and
Others Factors Affecting Occupational Health The list of factors which affect the occupational health and that need standard organising, scrutiny, inspection, surveillance and
Explain Casein Casein is an important example of protein, which can be boiled without apparent change in stability. The exceptional stability of casein makes it possible to boi
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