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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is the goal of risk assessment? The goal of risk assessment is to provide risk managers, who might be, for example, government regulatory officials, industry health a
a) The West of Scotland Coronary Prevention Study (WOSCOPS): The study randomized healthy men between the ages of 45 and 64 years with TC levels higher that 252 mg/dL and LDL chole
Hepaticae (Liverworts) The gametophyte is thalloid body or simple stem and leaves, with aquatic or semi-terrestrial habit. Rhizoids are multiceillular. Some examples : Mar
Why is holophytic nutrition a mode of autotrophic nutrition
Ciliates - Parasitic Protozoan The only ciliate of medical and veterinary importance is Balantidium coli which inhabit the large intestine of man, apes, monkeys and pigs. B. c
Q. Symptoms of inflammatory bowel disease? Inflammatory bowel diseases are associated with: • Abdominal cramping, diarrhoea • Steatorrhoea • Obstruction caused due t
Define Glycogenolysis Glycogenolysis : degradation of glycogen.
Define water losses by visible perspiration? The water losses by visible perspiration are highly variable; the amount could be as high as 4L in hot climate or during strenuous
life cycle of plathyhelmenthes
Define Diagnostic Criteria for Bulimia Nervosa? 1. Recurrent episodes of binge eating. An episode of binge eating is characterized by both of the following a) Eating, in a d
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