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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain about the Importance of Vitamins? Vitamins are the organic substances that act as coenzyme and/or regulator of metabolic processes. There are 13 known vitamins, most of
why can only one kind of cellulose be formed?
What are the morphological similarities and differences among nematodes and annelids? Nematodes, like annelids, have a cylindrical elongated body. Annelids differentiate from
Importance of vitamin D Vitamin D is mainly involved in the calcium and phosphate metabolism. It usually promotes absorption of calcium and inorganic phosphate from the intest
SIGNIFICANC E OF GASTRULATION - It results in the formation of three germ layers. It also resutls in the formation of different organs & organ systems. Gastrulation is e
Q. Symptoms and Complications of atherosclerosis? Symptoms Excessive weight, hypertension, high levels of cholesterol and triglycerides. Complications Myocardia
Q. Explain Spoilage of Sweetened condensed milk? The sweetened condensed milk contains about 8% milk fat, 23% total milk solids and sweetened with the addition of a sweetener,
Explain Antimicrobial Prophylaxis Antimicrobial prophylaxis can decrease the incidence of infection, particularly surgical site infection, after certain operations, but this be
Another possible way to classify organisms would be to separate them into unicellular and multicellular organisms. Explain why this is not a useful classification system. Other
Uterine Torsion Incidences of uterine torsions in cattle and buffaloes are not common. However, they are very common in heavy breeds and buffaloes and animals in slopy terrain
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