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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
These are safe and free of systemic side effects. However, gastrointestinal side effects are common, and compliance is poor. The average LDL decreases by approximately 15 per cent
Q. The vascular lesions caused by leeches upon the blood vessels of their host cause blood naturally to coagulate. How does the leech solve this problem since it could be expected
Define Voriconazole It is metabolized in the liver by CYP2C19, CYP2C9 and CYP3A4. CYP2C19 is genetically variant (about 3% to 5% of Caucasians and African-Americans and about 1
Define Calcium, Phosphorus & Vitamin D required for Elderly? As mentioned earlier, due to the change in skeletal system, vitamin D and calcium needs increase with age. Bone min
Q. Morphology in relation to taxonomy? Morphology is the basic tool of taxonomy, because identification is primarily based on the characters of the plant. The morphological cha
What are the hormones produced by the testicles and the ovaries? The testicles make androgenic hormones, the major of them being testosterone. The ovaries make estrogen and pro
Concomitant or Non-Paralytic Squnit This type of squint usually occurs when there is an obstacle in the sensory pathway of binocular reflex such as refractive errors, opacities
How can denaturizing be classified regarding its reversibility? Ans) Protein denaturizing can be a reversible or irreversible process, i.e., it can be possible or impossible to
Summary of the Phylum and protozoa and the important
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