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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
An ecosystem includes
S a f e ty, ethical and welfare considerations Development of transgenic organisms is proceeding in an environment of intense public debate about safety, environmental impa
Define the Historical Process of Spatial processes? In year 1952 Alan Turing developed his theory of the generation of pattern in systems of reaction diffusion equations, now k
Task: Write a maximum of five (5) pages of your research plan which should include details on the following topics as appropriate to your chosen study design: I. Background info
Which type Ribosome occurs exclusivley in Mitocondria?
phylum porifera
Terminology use in Apical Dominance Here are a few terms that will be used in discussing apical dominance. A clear understanding of these terms is needed for understanding the
Diagnosis Clinical signs: In most of the cases in initial stages like bacterial infections there is leucocytosis with neutrophilia, which at later stages of the disease may c
The entire mechanism of protein synthesis in eukaryotes is generally the same as in prokaryotes, with three phases explained as termination, elongation and initiation. Furthermore,
Types of Metamorphic Changes The process of metamorphosis includes reactivation of the morphogenetic processes. The morphogenetic changes also the mode of causation of these
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