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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Carbohydrate - contains Carbon , Hydrogen , and Oxygen with a general formula of C x (H 2 O) y Simple sugars à monosaccharides Oligosaccharide à sugars containing
Q. What are branchiae? What are examples of animals that "breath" through branchiae? Branchiae also known as gills are small portions of richly vascularized tissues external or
a) What are the two types of chemical compound which combine to form a lipid? b) What elements are present in a lipid? a) Lipids are formed from the combination of
what is a homologous chromosome
Explain Periimplant Marginal Tissues of Mucosal Conditions In addition to the redness and swelling of the marginal tissues, bleeding on probing (BOP), pocket formation, and sup
The vertebral bodies are much larger in the lower back than the neck. What is the functional significance of this structural difference?
Explain Nutrition or diet counseling Nutrition or diet counseling is a primary educational activity of the dietitian. It incorporates the idea of working with a patient, e
Define Metabolic Activity and Cell Constituents? Metabolic activity like amount of acid produced or CO 2 produced is directly proportional to the number of bacteria present, s
VACUOLES Some animals cells may have one to a few small fluid filled vacuoles.. Plants cells in general possess one or a few large vacuoles. Vacuoles of animals cells
Non-viral vectors Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods p
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