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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. What is the difference between micronutrients and macro? The classification criterion of nutrients into micronutrients and macro has no relation to the size of the molecul
EPIDIDYMIS - All seminiferous tubules form short straight tubules as tubuli recti, open into wider network of rete testis. From rete testis 15-20 fine convoluted tubules,
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#question.3 different genera of invertebrates
Q How is the large size of some cephalopods related to the kind of circulatory system they present? In cephalopods the circulatory system is closed and this provides more press
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Determine the types of symbols used in Soil Horizons Three kinds of symbols are used in various combinations to designate horizons. These are capital letters, lower case lett
Altitudinal Variations We know that temperature decreases with increasing altitude. This is mainly due to convection currents in the troposphere - the lowermost (and most dense
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