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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
GLUCOSE IS OXIDISED TO PRODUCE ENERGY TO GIVE ADP TO FORM ATP
Assignment of phylum chordata
Q. In what order should Personal Protection be removed? Personal protection is removed in following order: Gloves Mask Glasses Wash hands
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physical properties of protoplasm
Some ecosystems possess relatively large number of species as compared to others. This is particularly true of tropical forests which exhibit a very rich-biodiversity. For example,
What are the Results of pulmonary artery banding ? Results of surgery for ventricular septa1 defects are excellent. Though the mortality is not 0 per cent, in a large series it
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