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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is digestion? Digestion is the breaking down of larger organic molecules obtained from the diet, e.g. carbohydrates, fats, proteins, into smaller ones, as glucose, fatty a
In interspecific sterility, the failure in mating occurs because of inability of the sperm to reach the egg in animals and the pollen to reach ovules in plants. In plailts interspe
Results of Monohybrid Crosses Mendel got the following results from his monohybrid crosses :- 1. F 1 plants produced by a cross between two plants pure for the cont
Define the Prevalence and Incidence of bulimia nervosa? Bulimia nervosa appears to have become more prevalent during the past 30 years. We do not have much data on Indian popul
Why making a smoke print of a leaf
indicator for testing for the presence of sugar
Q. What are the major cells of the connective tissue proper? What is the name given to the intercellular material that surrounds these cells? The major cells of the connective
Amino Acids and Peptide Bonds, Protein Structure - The graduate can construct models of the structure and function of amino acids and peptide bonds, predict ionization of an amin
Which are the molecules that make possible active transport through membranes? Ans) Active transport is made by particular membrane proteins. These proteins are known as "pumps"
Explain about the Chemical Carcinogens? Chemicals have been shown to be carcinogenic. Some are naturally occurring components of plants and microbial organisms. Some are synthe
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