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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Distinguish between epithelial and connective tissues with respect to their cell arrangement? PROVIDE a specific example( for both tissue types) of how the arrangement of cells hel
TYPES OF SYNOVIAL JOINT - 1 . BALL AND SOCKET JOINT - One bone forms a ball like head that fits into a socket formed in the other bone. Movement is possible in al
Cephalopods - Feeding and Digestion in Molluscs Cephalopods are carnivorous. Tentacles or arms are food capturing organs. The number of tentacles changes in different cephalop
Q. What is proteinuria? Why is proteinuria a sign of glomerular renal injury? Proteinuria means losing of proteins through urine under normal conditions proteins are too big to
Why have biologists and bioinformaticians embraced the Web as a vehicle for disseminating data so quickly, whereas clinicians and clinical informaticians have been more hesitant to
Gas Exchange Gill surface area must be large enough to provide adequate exchange of gases. Therefore, highly active fish have largest relative gill area. Figure compares highl
Charles Darwin was the first person to suggest that: a. the present forms of all existing species are the result of natural selection b. species have changed over long periods of t
Evolutionary Implications of Natural Regulation Many changes in abundance can be attributed to changes in extrinsic factors such as weather, disease or predation. But some cha
Explain Fungi - Nutritional Types of Microorganisms? Fungi are filamentous, eukaryotic microorganisms, ubiquitous in nature. These grow best in dark and moist habitats. Their h
Initiation Codon is the codon at which the translation of a polypeptide chain is initiated. This is generally the first AUG triplet in the mRNA molecule from 5' end, where the rib
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