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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. What is Moderate level of Lactase activity? Intake of milk is restricted depending on the tolerance. Fermented and cooked form of milk should be preferred as it is better to
What is meant by bioassay? Name the two bioassays that are used to determine auxin activity in plants.
discovery of two light phase in photosynthesis
F o wl cholera Fowl cholera, a highly contagious disease of poultry caused by Pasteurella multocida, was one of the first infectious diseases to be recognized by Louis Past
Why is it rare to find hemophilic women? There are less hemophilic women than hemophilic men because women need to have two X chromosomes affected to develop the disease while
explain the genotypes for each blood type and how this is an example of codominance. Blood type- A, AB, B, 0
KREB CYCLE
Q. Which are the respiratory muscles in mammals? In mammals the muscles that participate in the breathing process are the diaphragm and the intercostal muscles, in respiratory
Hydrostatic Skeleton The functioning of the hydrostatic skeleton in an animal depends upon the musculature being arranged around an enclosed volume of fluid. After that, cont
Q. Explain Ciguatera Fish Poisoning? Ciguatera probably represents the most common cause of poisoning from fish in tropics. Ciguatera comprises over 50% of all reported case
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