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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
classification of invertebrate
Define Recommended Dietary Allowances for Vitamin D? The recommendations of vitamin D are expressed as IU. 1 IU is defined as the activity contained in 0.025 mcg of cholecalci
State the terms complete alimentary tract, complete digestive tract and complete gut? A digestive tract which has an anal and oral opening. This adaptation allowed for a linear
What are plant cell vacuoles? What are their functions? How is the covering membrane of the vacuoles called? Ans) Plant cell vacuoles are cell structures delimited by membranes
N a ture of viral diseases Viral diseases are manifested in acute, sub-acute or chronic forms, as frank clinical cases or as latent infections, some of which are fatal. Thes
Nervous System and Sense Organs The non-chordates also perform a variety of activities such as feeding, digestion, locomotion etc. For this aim, they have corresponding organs
Define measuring body composition- Total body potassium? It applies the' concept that body cells contain a constant amount of potassium and that its measurement is theref
definition of larva
what is allochory
Ecological taxonomy notes
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