Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

What proportion of the progeny, In holly, serrated leaves are dominant to s...

In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are

Intermediate filaments - role of cytoskeleton structures, Intermediate fila...

Intermediate filaments - Role of Cytoskeleton Structures These filaments are intermediate in size among microtubules and microfilaments and are 10 nm in diameter. Five classes

Grow Ligth Colors, What colors of light are needed in a grow light for stra...

What colors of light are needed in a grow light for strawberry development?

Transport of small molecules, The internal composition of the cell is maint...

The internal composition of the cell is maintained because the plasma membrane is selectively permeable to small molecules. A lot of biological molecules are unable to distribute b

Why are glucorticoids used in transplant patients, Q. Why are glucorticoids...

Q. Why are glucorticoids used in transplant patients? In the treatment and prevention of this common problem patients are given other immunosupressants or glucorticoids, Glucoc

Describe the advantages of sulphur, Describe the advantages of sulphur ...

Describe the advantages of sulphur Sulphur also increases the oil content of crops such as flax and soyabeans. Disulphide linkages ( -S-S-) have recently been associated with

What is the endocrine function of the placenta, What is the endocrine funct...

What is the endocrine function of the placenta? The placenta is not a permanent gland of the endocrine system but it also has endocrinal function. The placenta produces estroge

Define classification of carbohydrates - monosaccharide, Define Classificat...

Define Classification of Carbohydrates - Monosaccharide? Monosaccharides or simple sugars are those carbohydrates that cannot be hydrolyzed into simpler carbohydrates. E.g. gl

Organic chemistry and macromolecules, identify 7 speccific ways in which yo...

identify 7 speccific ways in which you can diversify carbon-containing compounds

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd