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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
An interesting observation made by an American biologist H.C. Bumpus (1899) provides a good explanation for normalising selection. Bumpus collected some 136 injured house sparrows
Q. Calorie requirements for ulcerative colitis? Energy: The calorie requirements must be increased to: 1) Restore weight status and maintain ideal weight. 2) Compensate f
How is the cerebrum anatomically divided? The cerebrum is divided into two cerebral hemispheres, the right and the left. Each hemisphere is made of four cerebral lobes: frontal
Which of the following is true for active hyperemia, a local control mechanism within the circulatory system? A. There will be an increase in force developed by smooth muscles
Im learning about difference cell structures in science. I was wondering if I might be able to get some information about the epithelial tissue, and the connective tissue. Thankyou
Determine Energy demand for Vigorous or vigorously active lifestyles? These people engage regularly in strenuous work or in strenuous leisure activities for several hours. Exam
The purpose of examining the abdomen should be for detection of renal artery bruit and abnormal kidney masses like kidney tumours and polycystic kidneys. Aortic abdominal aneurysms
What is the significance of the -R group (variable radical) in an amino acid molecule? The -R group, also known as a lateral chain, is the variable part of the amino acid molec
What is difference between self pollination and cross pollination? Which of the two modes of pollination contributes more to the plant diversity? Self pollination take places w
pls explan 2 puc lesson
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