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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Energy Input in Ecosystem Survival and functioning of ecosystem is dependent on the input of energy. Continuous availability of energy is essential for supporting diverse ecos
Your final assignment is to write an (~2000 words) on any drug that is currently approved by the FDA, describing how the drug works based on the mechanisms of human biology describ
Zygotic or Suspensor Polyembryony Cleavage of the apical cells of the globular or filamentous proembryo produced by the zygote may result in two or more embryos in a seed. Th
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Define regulation of L-PK enzymes by starvation & refeeding? L-PK catalyzes the conversion of phosphoenolpyruvate to pyruvate and plays an important role in directing pyruvate
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