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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
1. Explain how bats determine the distance (range) of their target using echolocation. Be sure to describe the properties of their call that make it possible (use a sketch), and ho
A decrease in parasympathetic discharge to the heart leads to A. a decrease in the conductance of F-channels in SA node cells. B. an increase in the conductance of potassium
Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitroc
Which is the brain region that receives conscious sensory information? Which is the brain region that triggers the voluntary motor activity? In the brain conscious sensory info
Nervous System of Echinoderms Previous to studying the nervous system of echinoderms, you have to bear in mind the peculiar organization of these animals. Here as well, the ne
Mutations are the only kind of variability generating mechanism in prokaryotes and asexually reproducing organisms. But in eukaryotes where sexual recombination occurs, even in one
Illustrate the Applications of Gellan gum Gellan is a highly versatile food ingredient and has numerous applications in foods. The combination of gelatin with gellan, by compar
The risk for IE among IV drug abusers, 2 to 5 per cent per patient- year, is estimated to be several fold greater than that of patients with rheumatic heart disease or prosthetic v
how is an increase in glucose concentration controlled in the human body?
Formation of pronuclei : Information of pronuclei, micronucles which is one of the conjugants, plays an important role. Micronucleus undergoes meiosis and forms four haplo
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