Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Horse diseases-borna disease, Borna disease Borna disease is fatal neurolo...

Borna disease Borna disease is fatal neurological disease of horses and sheep caused by a virus (RNA) of the family Bornaviridae and the disease is characterized by sub acute meni

What is the main cell organelle involved in cell digestion, What is the mai...

What is the main cell organelle involved in cell digestion? What are the properties of that organelle that enable it to do the task? The organelles responsible for intracellula

Chromosomes, how many arrs are present in mataphasic telocentric chromosome...

how many arrs are present in mataphasic telocentric chromosomes

What is glands, What is Glands? The central nervous system controls muc...

What is Glands? The central nervous system controls much of the endocrine system through the hypothalamus. The hypothalamus directs many of the body's functions through the "ma

Explain aspergillus - fungi, Explain Aspergillus - Fungi? Classificatio...

Explain Aspergillus - Fungi? Classification Kingdom - Mycetae Division - Amastigomycota Class - Ascomycetes Subclass - Plectomycetidae Order - Eurotiales Fami

PHYLUM ANNELIDA, GERENAL CHARACTERICS OF PHYLUM ANNELIDA

GERENAL CHARACTERICS OF PHYLUM ANNELIDA

What is genesis of coronary artery diseases, What is genesis of coronary ar...

What is genesis of coronary artery diseases ? Like any other disease conditions, origin of CAD depends on an interplay between the heredity and environment so called 'nature' a

How do derived characters help cladistic taxonomists, How do derived charac...

How do derived characters help cladistic taxonomists determine phylogenetic relationships? A derived character is found only between members of a particular group. Thus, cladi

What is electro osmosis , What is Electro osmosis ? Electro osmosis :...

What is Electro osmosis ? Electro osmosis :  Electroosmosis is the movement of charged molecules along a charged surfaces in response to a voltage difference. For example, e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd