Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Explain culture characteristics of molds, Q. Explain Culture Characteristic...

Q. Explain Culture Characteristics of Molds? The gross appearance of a mold growing on a food often is sufficient to indicate its class or order. Some molds are loose and fluff

Measurement and evaluation of food texture, Q. Measurement and Evaluation o...

Q. Measurement and Evaluation of Food Texture? Kinesthetic characteristics deal with the sense of feel, just like the characteristics of appearance deal with the sense of sight

Explain measurement of cell mass - microbial estimation, Explain Measuremen...

Explain Measurement of Cell Mass - Microbial Estimation? You may recall reading earlier that filamentous bacteria and moulds cannot be counted satisfactorily by employing plate

Explain the female reproductive system, Explain the Female Reproductive Sys...

Explain the Female Reproductive System? The female reproductive system consists of the primary sex organs, the ovaries, and the accessory organs necessary to effect fertilizati

What are the general symptoms of brain tumors, What are the general symptom...

What are the general symptoms of brain tumors The general symptoms of brain tumors, which result from increased intracranial pressure, include headache, vomiting, swelling of t

Determine about the term - neuropsychology, Determine about the term - neur...

Determine about the term - neuropsychology The clinical practice of neuropsychology involves an integration of knowledge bases from the disciplines of psychology, psychometrics

Explain the parental gametes and recombinant gametes, In genetic recombinat...

In genetic recombination by crossing over what is the difference between parental gametes and recombinant gametes? Parental gametes are those gametes that keep the original lin

What is the most likely genotype of p1, In cats, curled ears (Cu) results f...

In cats, curled ears (Cu) results from an allele that is dominant over an allele for normal ears (cu). Black colour results from an independently assorting allele (G) that is domin

Father, who is the father of science

who is the father of science

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd