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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Consequences of Aging It is hard to enumerate the general consequences of aging in different animals. Gradual loss of reproductive ability, neuromuscular coordination and not
Calculate the osmolarity of a solution of 5% dextrose in water (dextrose = glucose, molec. Wt. = 180).
List out the endothermic and exothermic reactions taking place in our body
Q What are the major ecological roles of bacteria? Bacteria are responsible for the decomposition process at the end of food webs and chains in this process they also liberate
Explain the Faecal Coliforms - Microbiological Study of Water? The faecal coliforms include a wide range of bacteria, many of which are not primarily the intestinal bacteria. T
Q. What is H shaped incision and what is it's purpose? This type of incision is especially useful for: The anterior maxilla or mandible. Accurately identified implant
Q. Plant classification according to Ancient Greeks and Romans? Hippocrates, "The Father of Medicine" (460-377 B.C.) is reputed to have been one of Democritus 's disciples. He
Orientation to the Paediatric Unit When the family arrives at the pediatric unit, they should be shown the room where their child will stay and the toilet facilities, the fan
J-shape d - It shows 2 phases - (i) Lay phase (ii) Exponential phase
The most abundant from of phosphorous has 15 protos, 16 neutrons and 15 electrons. Which of the following is an isotope of pgisphorous? (make all correct choices) A. an atom wit
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