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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is the structural flat representation of an amino acid molecule? An amino acid has a central carbon to which a carboxyl group binds on a side and to which a -R (variable r
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#question.endocrine organ in crustaceans
What do you mean by Advanced characters The traits, or characteristics, that an animal has that are not ancestral to the taxon. They appear later in the evolutionary history of
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State about Dental implant reconstruction Dental implant reconstruction requires a thorough knowledge and understanding of the various strategically important anatomic landmark
what is the difference between neurological and neurovascular observations
Q. What are the Communication process steps?Communication process steps: 1. Rapport Building 2. Finding the Problem 3. Planning 4. Implementation of the Plan 5. Ter
In designing an experiment to find out whether light is required for photosynthesis (a) what is the principle of the design (b) what control would you use?
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