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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
CARE OF RECORDS Patient's/client's records are unquestionably a valuable documents. These should be made on paper of good quality and protected from soiling, and burning. Th
Class of Subphylum Uniramia - Insecta Class Insecta consists of nearly a million described species. There are more species of insects as compared to all the other species of a
Ringworms (cutaneous mycoses) Cutaneous mycoses – also called dermatomycoses, occur worldwide and represent the most common fungal diseases in humans. The dermatophytes are th
What term is used to describe the range of organisms in a habitat
CELL MEMBRANE It is biological, thin, elastic, flexible & selective permeable membrane. Term "Cell membrane" was coined by Negeli & Crammer 1855. Term " Plasma membr
Why Physical Activity is important for weight loss? You are already aware that exercise plays an important role in initiating and sustaining weight loss along with dietary and
Explain about the Holmgren's Wools This is a matching test of coloured pieces of wool. While this is not a highly favored method of testing it does show serious d
Explain the Features of Alternaria? 1. Alternaria colony is wooly and compact. Underside is very dark coloured. Colony colour is grayish green or black with gray edges rapidly
COMMON RESPIRATORY DISORDERS: Respiration is one of the most vital functions of the body. The purpose of respiration is to provide oxygen ta the body cells and to remove exc
Explain the Chemical Methods for Control of Microorganisms? Different chemicals can be used which can act as disinfectant, antiseptics or even chemical sterilants. These are:
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