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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
what are the phase of alpha taxonomy?
Measurement of Nitrogenase Activity There are various methods to find out whether an organism is a N 2 -fixer or not. If an organism can grow in normal atmosphere without any
Explain the Etiology and Clinical Features of Parkinson's disease? The cause of Parkinson's is unknown. Genetic predisposition (in most cases the reason for the death of these
A cat has just arrived to the veterinary clinic with low blood pressure. The cat weighs 5 kg, and as the on call Vet you want to use a bag of saline to prepare a dopamine drip for
Define Nutrition Support in elderly - Parenteral, Enteral and Oral? Under nutrition either due to dietary deficit a due to a medical complication may arise in the elderly. The
Q. Why can mitochondria be considered the power plants of the aerobic cells? Mitochondria are the "power plants" of aerobic cells because within them the final stages of the ce
subject for botany assignment
CELL SURFACE JUNCTIONS (1 ) Desmosomes (Maculae adherence) - Thickened circular areas on P.M. of adjacent cells with radiating fibrils in cytoplasm. These fibrils calle
Why can it be said that each glucose molecule runs the Krebs cycle twice? Every glucose molecule "cycles" the Krebs cycle twice because after glycolysis each used glucose has g
Enterocoelous pattern – Coelom In enterocoelous development of coelom the mesoderm arises in the embryo as paired lateral pouches growing out from the archenteron. The pouche
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