Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

What is glycogen metabolism, Glycogen is a multibranched polysaccharide whi...

Glycogen is a multibranched polysaccharide which serves as a part of energy storage in fungi and animals. In humans the glycogen is recognized and stored primarily in the cells of

Explain assessment of iron status - serum ferritin, Explain assessment of i...

Explain assessment of iron status - Serum Ferritin? Serum Ferritin: This method is indicative of iron stores. As we know, a long term negative iron balance first results in dep

Nature and distribution of viruses, Nature and Distribution of Viruses ...

Nature and Distribution of Viruses Although several infectious diseases of animals and plants had been found to be caused by viruses during early 20 th century, the true natur

Myocarditis, Myocarditis Myocarditis is inflammation of the myocardiu...

Myocarditis Myocarditis is inflammation of the myocardium, of the heart muscles. i) Causes Infection: . Wrus, bacteria, fungus, parasites Non-infection: Radi

Rehabilitation of cardiac surgical patients, REHABILITATION OF CARDIAC SURG...

REHABILITATION OF CARDIAC SURGICAL PATIENTS The goal of nursing care of cardiac surgical patient is to make the patient to come back to his normal activities. Cardiac surgica

What are extraembryonic membranes, What are extraembryonic membranes? E...

What are extraembryonic membranes? Extraembryonic membranes are membranous structures that appear in parallel with the embryo and play significant roles in the embryonic develo

Ilustrate the nutritional and functional role of sodium, Minerals  :-  Sodi...

Minerals  :-  Sodium Food Source      NaCl, MSG, other food additives, milk, low in most raw foods Nutritional Functional role Essential nutrient: Deficiency is ra

Describe the several steps by allosteric inhibition, Which of the following...

Which of the following are features of allosteric inhibition? During the infection, there are many different environments that P. multocida will encounter. In order to cause an inf

Pollution, Phosphate detergent as cause of water pollution

Phosphate detergent as cause of water pollution

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd