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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Explain the Process of Canning? The basic principles of canning have not changed dramatically since Nicholas Appert and Peter Durand developed the process. Heat sufficient to d
TEN EXAMPLES OF ZOOFLAGELLATES
DB is a standard abbreviation used for the quantitative expression of: 1. the density of bacteria in a medium 2. a particular pollutant 3. the dominant Bacillus in a cultu
How plants solve the problem of transporting substances throughout their tissues? In the bryophytes the substance transport is done by the diffusion. In the tracheophytes (pter
what are pathogenic characteristics of protoctista?
Steps for Prevention Industrial Accidents These steps are as follow: Substitute a less hazardous equivalent. Remove worker from hazard (e.g. automate the sys
Practical Points in Preparation of Nursing Care Plan You first review the nursing diagnosis, then assign this priority or the order in which these are to be met. Alo
Degrade a monoglyceride that has an 18-carbon fatty acid attached to it by Ester bonds. You will have to degrade the glycerol component followed by the fatty acid in presence of O2
Ask question what is classification of livibg thingsMinimum 100 words accepted#
Braxy The causative agent of braxy is Cl. septicum. It usually affects lambs. The agent is a normal inhabitant of soil and is frequently found in the faeces of herbivores. Bra
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