Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

What is coronary bypass graft, Q. What is coronary bypass graft? The Co...

Q. What is coronary bypass graft? The Coronary artery bypass graft is a kind of surgical myocardial revascularization that is a way to provide blood to a myocardium whose blood

Birth control - temporary methods, BIRT H CONTROL - Birth control meth...

BIRT H CONTROL - Birth control methods which deliberately prevent fertilization are contraception. These methods may be temporary or permanent. 1 .       TEMPORARY MET

Define about the nutritional deficiencies, Define about the nutritional def...

Define about the nutritional deficiencies? The nutritional deficiencies which have been identified in the group of elderly are of total calories, protein, vitamin A, vitamin C,

Retina, Retina As invagination of the optic vesicle carries on to form...

Retina As invagination of the optic vesicle carries on to form the double walled optic cup the two layers of the optic cup start to differentiate in different directions. Pigm

What is the difference among plasma membrane and cell wall, Plasma membrane...

Plasma membrane and cell wall is not the similar thing. Plasma membrane, also known as cell membrane, is the outer membrane common to all living cells and it is made of a phospholi

Explain the uses of isp in meat products, Explain the Uses of ISP in meat p...

Explain the Uses of ISP in meat products Meat products  The main application of ISP in connection with meat and related products is based on the use of texturized ISP, in

Fibre requirement in chronic diarrhoea, Q. Fibre requirement in chronic dia...

Q. Fibre requirement in chronic diarrhoea? Fibre: Insoluble fibre in the form of skins, seeds and structural plant materials should be strictly avoided to minimize on the irrit

Absorption of glucose, Normal 0 false false false EN-IN...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Describe the frequency of low-risk coronary patients, Describe the Frequenc...

Describe the Frequency of low-risk coronary patients? For many low-risk coronary patients, particularly those following myocardial revascularization procedures, rehabilitation

Indian tick typhus, Indian tick typhus Indian tick typhus (Mediterrane...

Indian tick typhus Indian tick typhus (Mediterranean spotted fever) is a tick-borne rickettsial infection caused by Rickettsia conori and is characterized by fever and a chara

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd