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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
How did Pasteur's experiment vary from Spallanzani's experiment? Instead of sealing the flask in the experimental group after boiling, Pasteur used a flask with a curved neck,
Explain about the Epilepsy? Epilepsy is a neuromuscular disorder in which transient seizures recur due to an abnormal brain activity. The brain, through all orderly electric ex
Define Endurance capacity by standard running tests? Endurance capacity, on the other hand, is measured by standard running tests in which time to exhaustion is taken as the en
Q. Why does thymine replace uracil in DNA? The difference between DNA and RNA is existence of a hydroxyl (-OH) group on 2' carbon of the ribose sugar in the backbone. Removal o
Explain nutritional properties of the eggs Drying of eggs under normal conditions causes little loss of the nutritional properties of the eggs. Vitamin A, vitamin B, thiamine,
How will the plant-incorporated protectants rules change EPA's current registration process? The rules clarify which plant-incorporated protectants are needed to be evaluated u
how do i find an atomic structure
You have two enzymes, 1 and 2. Both convert substance A into substance B. 1 is inhibited by B, because B partially blocks the active site and will not allow more A to enter. 2 is i
C o l l i ba c i ll o si s It is commonly seen in newly born farm animals and occurs in septicaemic and enteric collibacillosis forms. E tiology: The disea
MIDDLE LAMELLA It is thin, amorphous, intercellular matrix between 2 adjacent plant cell. The cells of plant tissue generally cemented together by an intercellular matrix
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