Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

How much fluids should be taken for management of obesity, How much Fluids ...

How much Fluids should be taken for management of obesity? Liberal amounts of water and zero/low calorie fluids may be included in the diet. It may be helpful to have a glass o

How can the enzymes be classified, How can the enzymes be classified? Expla...

How can the enzymes be classified? Explain giving examples. Based on structure, enzymes can be classified into monomeric enzymes and oligomeric compounds. Monomeric enzym

Define the interaction of vitamin c with other nutrients, Define the Intera...

Define the Interaction of Vitamin C with other Nutrients? Having gone through the discussion above it must be evident to you that nutrients are interdependent and are related t

Digestive system, describe secretory function of salivary gland

describe secretory function of salivary gland

Virus, assignment order

assignment order

Necrotic enteritis, N e c r otic enteritis It can be designated as ...

N e c r otic enteritis It can be designated as enterotoxaemia of chickens, turkeys and ducks caused by Clostridium perfringens. The toxin released by this spore-bearing a

Effects on weather - air pollutants, Effects on Weather - Air pollutants ...

Effects on Weather - Air pollutants Dust, smoke and other suspended particulate matter reduce visibility. Fly-ash also affects visibility by intercepting and scattering solar

Embryo., Few sentences about embryo

Few sentences about embryo

Baby delivery, some deliveries of baby occur early na. y is it so?

some deliveries of baby occur early na. y is it so?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd