Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Name mimicry by which insect mimics insec for protection, From the followin...

From the following, name the type of mimicry by which a palatable, acceptable insect mimics an unfavorable, noxious insec for protection. a) Batesian mimicry (pron: BATE-see-an

Explain in detail about the optic nerve, Explain in detail about the optic ...

Explain in detail about the optic nerve The optic nerve contains more than one million axons that initiate in the ganglion cell layer of the retina. This structure originatcs a

Define classification of lipids, Define Classification of Lipids? Chemi...

Define Classification of Lipids? Chemically, lipids are the organic molecules poor in oxygen content, soluble in organic solvents but insoluble in water. They are classified as

What is the etiological agent of cutaneous leishmaniasis, Q. What is the et...

Q. What is the etiological agent of cutaneous leishmaniasis? How is the disease transmitted and what are its typical manifestations? The protozoan Leishmania braziliensis is et

How genes can be exchanged between chromatids, Q. How Genes can be exchange...

Q. How Genes can be exchanged between chromatids? When two homologous chromosomes physically exchangecorresponding segments during prophase I of meiosis, geneticists call it cr

What are the stages into which photosynthesis is divided, What are the stag...

What are the stages into which photosynthesis is divided? Photosynthesis is separated into the photochemical stage, or light reactions, and the chemical stage. Photosynthesi

What is phylum platyhelminthes - flatworms, What is Phylum Platyhelminthes ...

What is Phylum Platyhelminthes - Flatworms ? This Class consists of the free-living flatworms, the best known of which is the Genus Planaria. Most of the species in this class

Difference between the concepts of karyotype and genome, What is the differ...

What is the difference between the concepts of karyotype and genome? Genome is the set of DNA molecules that characterizes each living being or every species. The concept then

What are the techniques of pumonary embolism operations, What are the techn...

What are the techniques of pumonary embolism operations ? Technique of Operation :  Patients who are very unstable will have to be put on bypass by cannulation of femoral art

How many distinct hexapeptides made from 20 amino acids, How many distinct ...

How many distinct hexapeptides can be made from 20 amino acids?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd