Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

Fission - asexual reproduction, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Are there ribosomes in a plant cell, Are there ribosomes in a plant cell? ...

Are there ribosomes in a plant cell? Yes, in fact there are ribosomes in both plant and animal cells. That is right. Ribosomes are the site of synthesis of polypeptide chain

Explain the nucleic acid is the transforming agent or not, Fredrick Griffit...

Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following prevented him from being able to decl

Show the energy pyramids, Q. Can the amount of available energy in a given ...

Q. Can the amount of available energy in a given trophic level be larger than the available energy in inferior trophic levels? What does that condition means to the conformation of

Pedigree charts, i need help with to do a pedigree charts.

i need help with to do a pedigree charts.

Explain lane eynon method procedure, Q. Explain Lane Eynon Method procedure...

Q. Explain Lane Eynon Method procedure? You will be carrying out the procedure in two steps using Lane Eynon Method. Step 1 It involves the standardization of copper s

What happens to the parent cell, What is produced at the end of the cell cy...

What is produced at the end of the cell cycle? how do they compare to each other and to the parent cell? What happens to the parent cell?

Excretion, living organisms and their excretory products

living organisms and their excretory products

Explain denaturation and coagulation, Explain Denaturation and coagulation ...

Explain Denaturation and coagulation Denaturation and coagulation of the proteins are considered to be the chemical changes, since they involve the unfolding of proteins, whic

Show characteristics of food products, Q. Show characteristics of food prod...

Q. Show characteristics of food products? These sensory characteristics of food products. Food quality, as such, can be divided into three main categories i.e. i. Appeara

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd