Explain the primary stain - ziehl-neelsen method, Biology

Assignment Help:

Explain the Primary Stain - Ziehl-Neelsen Method?

Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.


Related Discussions:- Explain the primary stain - ziehl-neelsen method

How can a child become infected with gonorrhoea, How can a baby become infe...

How can a baby become infected with (a) gonorrhoea, (b) syphilis   (a)  During birth, a baby might be become infected with gonorrhoea bacteria as it passes by t

Explain binding of pigments - proteins, Binding of  Pigments, synthetic dy...

Binding of  Pigments, synthetic dye In  addition  to water, lipids and  volatile  flavours,  food proteins  can  bind  a number of other  substances  by  weak interactions  or

Define diabetic ketoacidosis, Q. Define Diabetic Ketoacidosis? Diabetic...

Q. Define Diabetic Ketoacidosis? Diabetic Ketoacidosis (DKA) is one of the acute complications of diabetes mellitus. The name itself implies that there is acidosis (decrease in

Blood coagulation factor - circulation, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Indications for surgery-pericardial diseases, Indications for Surgery :  O...

Indications for Surgery :  Once diagnosis of constrictive pericarditis is made and confirmed by chest X-ray, ECG, echocardiogram, CT, MRI scan, cardiac catheterisation and angio,

How does the excretory system of nematodes work, Q How does the excretory s...

Q How does the excretory system of nematodes work? The metabolic residuals of nematodes are together by two longitudinal lateral excretory channels that open in one single excr

Determine about the diseases sleep attacks, Determine about the diseases Sl...

Determine about the diseases Sleep attacks These are brief, often irresistible, episodes of sleep, probably slow wave, NREM, naplike sleep that last about 15 minutes and can oc

Fertilization - development biology, Fertilization - Development Biology ...

Fertilization - Development Biology Previously you knew the process which leads to the differentiation of the male and female germ cells, the sperm and ova, respectively. In t

What is endocytosis , Endocytosis is the uptake of extracellular macr...

Endocytosis is the uptake of extracellular macromolecules   across the plasma membrane into the cell. The objects to be ingested is progressively  enclosed by a  small  portion  of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd