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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Which type of reproductive structure has a better chance of survival if there was a sudden temperature change in its environment?
Explain the Importance of Biochemical Tests? Specific series of biochemical tests have been developed for fast identification of microorganisms in laboratories. These biochemic
Rifaximin (Xifaxan) An anon-absorbed oral antibiotic, was about as effective as ciprofloxacin for treatment of travelers' diarrhea, mostly caused by E. coli. It is not effecti
Explain Electron Volt Electron Volt: Amount of kinetic energy obtained by an electron, initially at rest, after undergoing an acceleration by a one volt potential difference.
Define Conservation genetics and hybridization? Is the genetic integrity of European wolves threatened by hybridization along with free-ranging dogs? To ascertain the extent of
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Explain the types of foods used in Space? The various types of foods are enumerated herewith. Thennostabilized (T): Heat processed foods ("off-the-shelf' items) in aluminium
How is the nervous system characterized in beings of the phylum Annelida? How can one compare cephalization in annelids to cephalization in nematodes and platyhelminthes? Annel
Define requirements of Sodium during pregnancy period? As you know, sodium plays a role in fluid balance. Sodium metabolism is altered during pregnancy under the stimulus of a
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