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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
what is vaso-constriction
As in several fields of molecular biology studies of E. coli have gives the model for subsequent investigations of transcription in eukaryotic cells. The basic mechanisms by that t
Elaborates Class Trematoda and Monogenea - Flukes? Members of this Class are commonly known as the tapeworms. They resemble a long flat tape, or ribbon, and are parasitic, livi
Explain the Regulation of Blood Glucose Concentration? A number of mechanisms function to maintain blood glucose at remarkably constant level of 70-100 mg/dl under fasting cond
The Polymerase Chain Reaction Kary Mullis established the technique of the polymerase chain reaction (PCR) in the year of 1983. He won a Nobel Prize for the procedure in the ye
ECG: Myocardial ischemia is indicated by T-wave inversions. Left ventricular hypertrophy (LVH) criteria: R in aVL >13 mm R voltage in L1+ S III >25 mm S v1 + R v5 or v6
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The Physical Environment The actual hospital environment is another factor that may be stressful for the child and family. There are many unfamiliar sights, sounds and smells
Which of the following best explains the reason why DNA ligase is needed to complete the replication of internal chromosomal segments? A. DNA ligase is able to delete the last
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