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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Why is the cytoplasm of sperm cells very reduced? Why do mitochondria of sperm cells concentrate in the base of the flagellum ? The decreased cytoplasm of sperm cells reduces th
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It contain four steps. They are: Attachment to host Proliferation Invasion of host tissue Toxin-induced damage to host cell
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Many of the free energy free during the oxidation of glucose to CO 2 is retained in the decreasing coenzymes NADH and FADH 2 producing while glycolysis and the citric acid cycle.
Skeletal muscle A. The myosin head is activated (energized) by the conversion of GTP (that is bound to myosin) to GDP and Pi (that are bound to myosin). B. Detachment of the
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You are studying a gene, and its effects on skin cells. You want to find out A) If the protein made encoded by this gene binds to DNA in a particular place B) if the protein binds
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