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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What are lichens? How do fungi participate in this ecological interaction? Lichens are produced by mutualist ecological interaction among fungi and algae or among fungi and cya
a) What is meant by 'vaso-dilation'? b) What are the effects of vaso-dilation in the skin? (a) Vaso-dilation is an enhance in diameter of small arterioles and
Q. What are the complications of chronic dyspepsia? Complications of dyspepsia are listed below: • Wright loss: Since eating most of tell provokes the symptoms, patients res
pioneers of developmental biology and their contribution
What is Genetic Recombination and Evolution ? Variation among the offspring of living organisms results from genetic recombination through sexual reproduction and mutation. Va
Q. Illustrate the Methods to evaluate osseointegration? The criteria of success in implant dentistry remains complex. The vast majority of clinical reports noting success and f
Lateral Roots - Root Apex Lateral roots normally arise at a definite distance behind the tip from areas close to or opposite the points of xylem star. So, a triarch root can
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Name three characteristics of mitochondria and chloroplasts that support the endosymbiotic hypothesis of eukaryotic evolution. Both organelles replicate independently of the ce
Define the Structure of D and L isomerism? The prefix D or L are used to refer to the configuration of the carbon next to the primary alcoholic group or in other words, the con
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