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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Define about the Deficiency of cyanocobalamin? Malabsorption of vitamin B 12 can occur at several points during digestion. By far, the most important condition resulting in v
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Q. Factors Affecting Normal Blood Sugar Levels? Earlier in our discussion, we learnt that insulin is the most important factor responsible for normal blood sugar levels. Now we
As heart failure sets in, there is activation of RAS (Fig 2.1). Adrenergic stimulation of beta-1 receptors in juxtaglomerular apparatus of the kidneys results in release of renin.
Describe Splitting sequence of S 2 ? Even though the pulmonary valve closes at about 20 mm Hg and aortic valve at 100 mm Hg, the aortic closure occurs first as the impendence
living and non living
Explain the term - Migraine Perhaps the most common neurological disorder, migraine afflicts some 5% to 20% of the population at some time in their lives. The World Federation
Q. Alcohol consumption by diabetes patient? Intake of alcohol should be limited. It is high in calories, lacks essential nutrients and may therefore promote ketoacidosis, hyper
In a cohort study that found an association between alcohol consumption and bladder cancer, 20,000 middle-aged men were asked about their drinking habits and then tracked for five
Describe frequently respiratory infactions to recoganize congenital heart disease ? Frequent Respiratory Infections: Respiratory infections that are frequent, severe and diffic
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