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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is the meaning of term - Plasticity The notion of brain plasticity has been of interest to researchers and clinicians alike for decades. The outcome of injury is the resul
External Respiration: Diffusion of oxygen into blood and carbon dioxide into alveoli (external respiration) is the diffusion of oxygen from air in the alveoli of lungs to bl
Nucleic Acids Friedrich Miescher (1868) discovered the presence of these compound in protoplasm ,but Altman (1889) introduced the term Nucleic acid. These acids are the lar
What are some industrial processes that use bacteria? Bacteria are used by industry in many ways. There are vaccines made of attenuated pathogenic bacteria or of antigens prese
Liverworts and mosses are characterized by their lack of vascular conducting tissue. These two groups of liverwort, plants and mosses, are known by which of the below terms: a)
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about how humans survive and reproduce currently. In your journal, write down three adaptations that help humans have differential survivability, and three adaptations that help hu
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Abscisic Acid (AbA) - Plant Growth Substances In 1961 Lice and Cams isolated a substance in crystalline form from mature cotton fruit. This substance stimulated the abscission
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