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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Whenever a pair of alleles has different alleles is there dominance between them? Not in all cases of a gene includes two different alleles is the dominance complete. There are
The importance of the brain in our everyday lives can never be underestimated. The brain has physical properties that are in a constant state of flux. The brain never rests totally
The treatment of hyperlipidemia requires two approaches: Therapeutic lifestyle changes (TLC) and medications. To achieve target LDL levels, most patients need both approaches si
Define Determinants of Food Security - Food Access? Food access is linked to its affordability. Food access is ensured when households and all individuals within them have adeq
Define needs of Vitamins arid Minerals in Postoperative Nutritional Care? Vitamins are required for wound healing after a surgery. Vitamin C provides the cementing material of
COMMON EQUINE DISEASES - 1 . INFLUENZA OR PINK EYE - Pink eyes. Swallon eye lids. 2. STRANGLES - Fever, dullness, depression, nasal discharge, lumpy a
Q. Etiologic factor of Congestive Cardiac Failure? Congestive heart failure develops over a period of time when the necrotic tissues are not replaced by functional connective c
a) How is the milk production regulated by hormones in human female? Define. b) Explain the importance of the auxin / cytokinin ratio in plant tissue culture.
what cell organelle does photosynthesis take place in?
Aeration is the process on which water and air come into contact. It is carried out by spraying water in the form of spray or fountain over air. Aeration achieves the following obj
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