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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
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can you give brief diagram of the tracheal system of cockroach?
Define Abbe Condenser of Microscope? It is present beneath the stage, as shown in Figure. It collects and focuses a cone of light on the slide. Its position can be adjusted ver
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General Characters-
What are the major functions of the organic molecules for living beings? Organic molecules, such as proteins, lipids and carbohydrates, perform numerous functions for living or
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Explain Direct Microscopic Count - Measurement of Microbes? The most obvious way to determine microbial number is through direct counting under a microscope. In this method, a
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