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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Tests of shelled amoebas are made from a variety of substances including sand grains, chitin and substrate, calcium salts, and even silica. These shells still accumulate in ocean d
why is it known as middle man
Define SEPSIS - Nutrition during Stress? Sepsis is defined as the presence of an infection due to an identifiable organism. Bacteria and their toxins lead to a strong inflammat
(a) For any individual : · We should check house, doors and windows and have them repaired whenever necessary. · We shou
It is found in around 20 per cent cases of ARF and it is a late manifestation occurring even 3 months after GAS pharyngeal infection. Chorea is triggered by emotional disturbances
Define Energy Requirement for Cancer Patients? It must be clear to you that cancer imposes increased energy demands because of the hyper-metabolic state of the disease process
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DIFFERENCE BETWEEN LIVING AND NON-LIVING BEINGS - S .No. C h a ra cte r L i v i n g be ings N o n - l i v i n g be
A woman is heterozygous for three X-linked loci: AaBbDd. She has seven sons with the following genotypes: two are X ABd Y three are X abD Y one is X ABD Y one is X Abd
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