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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Vitamin B 2 (Riboflavin) Riboflavin is a yellow to orange-yellow, crystalline powder of faint odour and intensely bitter taste. Its solubility in water is very slight and depe
Importance of fat synthesis and storage : As is clear from above account, all surplus nutrients, digested and absorbed from above the immediate need of body, are readily
How are human voices created? Human voice is formed with the help of muscles in the neck and the vocal cords. The tighter the vocal cords the higher the pitch of voice. This is
PAEDIATRIC NURSING Parent education, family health promotion and health maintenance have been the concern of yesterday's and today's nurses. Florence Nightingale over a hu
What is reflexive memory? Reflexive memory relies on the cerebellum and amygdala. Compare among aerobic respiration and anaerobic respiration. Aerobic respiration happens in th
Phenoxyethanol is dangerous and can be absorbed through the skin - official sites for toxicity data, though, show little toxicity in man and some toxicity (irritation) with high do
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State the term in detail hydrostatic skeleton. Formed from a fluid-filled and closed cavity surrounded by a body wall comprising muscles oriented in different directions. Muscu
A simple osmometer Remove the bottom from a small glass bottle about 2.5 cm in diameter. Fit a one-hole stopper tightly into the bottom and put a 50 cm length of glass tubing o
Necessity of interdependence of Volvox organisms in the colonial existence
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