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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
list the long-term and short-term effects of forest destruction (a)on hillsides,(b)in the tropics?
Define Tests for the Presence of Exoenzymatic Activity? Microorganisms require various micro- and macro-nutrients for energy production and growth. These are obtained from the
Q. How are the major classes of arthropods classified according to the presence of larval stage in their embryonic development? In crustaceans there are others with indirect an
What is the Results of Congenital Pulmonary Stenosis? Early mortality for open pulmonary valvotomy in neonates varies between 6 and 10 per cent. For infants and children, surgi
If a cell were placed in a hypertonic solution what could it do to protect itself?
What is L forms of bacteria?
Explain solid salt and saturated aqueous solution? In this example, the equilibrium system consists of crystalline PbCl 2 and an aqueous phase containing the species H 2 O, P
Define Health and Feeding Problems among Elderly? Malnutrition, both obesity and under nutrition, are common problems linked with elderly. Some of the common disorders such as
what are the component of hemoglobin
Explain The Estimated Average Intake (EAR)? Considering that the nutrient requirements follow a normal distribution, the EAR is defined as 'the median usual intake that meets t
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