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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
how pollination occurs in Salvia?
What proportion of children with Down syndrome do you expect when women with Down syndrome have children with men who have 46 chromosomes justify your answer?
Scrapie This is a chronic fatal disease of sheep and occasionally of goats caused by a Prion agent similar to BSE. The disease is reported from Europe, North America and in a f
Anima l Nutrition Nutrition is the procurement of substances necessary for growth, maintenance and activities of a living body. The substances used for nutrition are called
what is a homologous chromosome
Database Search: Once an open reading frame or the partial amino acid sequence has been pre-determined, the investigator compares sequence with others in the databases using a com
A person with a cough takes a patent cough syrup. In three days, the cough is better. Does this mean that the syrup has cured the cough? Justify your answer. There is insuffic
Q. Why are glucorticoids used in transplant patients? In the treatment and prevention of this common problem patients are given other immunosupressants or glucorticoids, Glucoc
What capacity does the brain have to repair itself after injury or disease? It has long been accepted dogma that central nervous system doesn't regenerate itself after traumati
Q. Besides vertebrates two invertebrate phyla contain species that make pulmonary respiration. Which are these phyla? Terrestrial molluscs and the arachnid arthropods are the i
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