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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What are the endocrine glands involved in the menstrual cycle? What are the hormones in action? The endocrine glands that secrete hormones included in the menstrual cycle are t
how do i find an atomic structure
Explain E. coli - Microbiological Study of Water? It is always present in human intestine so its presence indicates the faecal pollution and possible presence of other human or
Who is known as Father of English Botany? William Turner (1515-1568), whose 'A New Herbal' printed in English appeared in 1551 (first part), 1562 (second part) and 1568 (third
how does sodium and potassium ions maintain the resting petential of neurons
Define Physiological and Socio Psychological Factors - public nutrition? Food related behaviour depends on a combination of biochemical factors, mainly, physiological aspects a
Q. How Boiling used for sterilization? Though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often. Boiling water in hot wa
Write short note on glycogen storage diseases. Glycogen storage diseases are caused by genetic defects that result in deficiencies in certain enzymes of glycogen metabolism.
Occurrence Pantothenic acid Pantothenic acid is a ubiquitous active principle occurring in free as well as in the bound form. In the bound form, pantothenic acid is incorpor
Planes of Cleavage The ova of most of the animal groups (except some specific cases like insects) are spherical or nearly spherical having their own actual centre comparable
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