Explain the most probable number (mpn) techniques, Biology

Assignment Help:

Explain the Most Probable Number (MPN) Techniques?

Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.

Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.


Related Discussions:- Explain the most probable number (mpn) techniques

Prevention - rheumatic fever, Acute GAS pharyngitis should be treated promp...

Acute GAS pharyngitis should be treated promptly by penicillin or other antibiotics. Indian Council of Medical Research (ICMR) recommends that all cases of pharyngitis must receive

Respiration in animals, Respiration Robert Boyel and Robert Hook fir...

Respiration Robert Boyel and Robert Hook first of all explained the meaning of respiration. Main aim of respiration is to liberate energy in the form of A.T.P. Oxidati

Explain the development of cancer, Explain the Development of Cancer? A...

Explain the Development of Cancer? Are you interested in knowing how cancer develops? Well here it is. The cancer development is a process involving initiation, promotion and p

Rotential complications of post removal, Rotential complications of post re...

Rotential complications of post removal -Fracture of the tooth , -leaving the tooth unrestorable , or restored with fair prognosis -root perforation , -post breakage, and -

What are the biotolerant materials, What are the Biotolerant materials ...

What are the Biotolerant materials Biotolerant materials, are characterized by a thin fibrous tissue interface. The fibrous tissue layer develops as a result of the chemical pr

Phylum protozoa, Classification of the phylum protozoa

Classification of the phylum protozoa

Determine the food source for phosphorus, Determine the Food Source for Pho...

Determine the Food Source for Phosphorus? Phosphorus is widely distributed in food. Food phosphorus is a mixture of both organic and inorganic forms although the relative amoun

Acute bronchitis, Acute Bronchitis: Acute bronchitis is inflammation  ...

Acute Bronchitis: Acute bronchitis is inflammation  of one or more bronchi  and occurs specifically  in children younger than  four year?.  It is usually associated with previ

How does aldosterone act and where is it produced, How does aldosterone act...

How does aldosterone act and where is it produced? Aldosterone is a hormone that acts upon the nephron tubules stimulating the resorption of sodium. Thus it contributes to the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd