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Explain the Most Probable Number (MPN) Techniques?
Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.
Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.
Explain Problems of Infants and Preschoolers Nutrition? In spite of all nutrition interventions, there are some of the common yet life threatening problems which need to be loo
a) In humans, is it the sperm or the ovum which verifies the sex of the offspring? b) Give a reason for your answer. (a) The sperm verifies the sex of the off
How much salt is in the human body? 50 Kilo human has about 7 tablespoons of salt inside there body.
Acute Mitral Regurgitation : Acute mitral regurgitation is an indication for early surgery. If patient is haemodynamically unstable, pre-operative intra aortic balloon pump
What is the structure that maintains identical chromatids bound? Ans) The structure that handles identical chromatids bound is the centromere.
What is Fermentation ? Fermentation is a process used by anaerobic organisms and certain cells of aerobic organisms, such as muscle cells deprived of oxygen, to obtain addition
List the advantages and disadvantages of stage 2 surgery using a tissue punch. Advantages - Less traumatic to surrounding tissue - Faster progress to the impression proce
Explain the Pre Pregnancy Height and Foetal Outcome? Maternal height prior to conception, determined by heredity, socio-economic environment and maternal nutritional status, is
The destruction of the ozone layer may be responsible for an increase in
Determine the Types of Exercises Exercises are generally grouped into three types: - Flexibility exercises such as stretching improves the range of motion of muscles and jo
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