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Explain the Most Probable Number (MPN) Techniques?
Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.
Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.
Describe Pathophysiology of tetralogy of fallots ? The PS causes concentric hypertrophy of right ventricle without enlargement. The right ventricular pressure are equal to or h
How Phosphorus in Blood is presented? Phosphorus in Blood is Present as: 1. Inorganic phosphorus in the form of HPO 4 or H 2 PO 4 2. Organic or ester phosphorus such
a) The image above is part of a DNA sequencing gel. Assuming this is the DNA coding strand and you are reading 5' -> 3' what are ALL the possible polypeptides this sequence cou
Define Fats required for underweight - nutritional care? Fats: We know that fats are concentrated source of energy (lg = 9 Kcals). Fats are capable of increasing the energy val
Define Functions of Protein? Each of the various proteins in the body serves a specific function in the maintenance of life. Any loss in body protein, in reality, means a loss
aspergillosis
Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-
Coronary Vasoconstriction 1) Coronary flow limiting stenoses are caused by concentric or eccentric artherosclerotic plaques, with or without potential for local vasomotor change
Gum Tragacanth Gum tragacanth, the exudation of Astragalus species, is defined as the "dried gummy exudate" obtained from Astragalus gummifer Labillardiere. Gum tragacanth is
What do you understand by Mesocoel? The middle of three coelomic spaces found in tripartate body plan characteristic of deuterostome lineage of animals. Other coelomic compartm
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