Explain the most probable number (mpn) techniques, Biology

Assignment Help:

Explain the Most Probable Number (MPN) Techniques?

Different procedures and media may be used depending on the food tested and the information sought. Media which can be used include Brilliant green lactose bile broth, Eosin methylene blue agar, lauryl sulfate tryptose broth, violet red bile agar or lactose broth etc. Presumptive count of coliforms in food is determined using Lauryl Sulphate Tryptose (LST) broth and Violet Red Bile (VRB) agar.

Former helps in detection of microbes that produces gas from lactose (gas in Durham tube) while acid production is detected by VRB agar (purple red colonies surrounded with a precipitate). Additional testing is done by inoculating from the positive presumptive tests into Brilliant Green Lactose Bile (BGLB) broth for confirmation. Gas in Durham tube confirms the test. Samples from the positive confirmed test are then streaked on to Eosin Methylene Blue (EMB) agar for completing the test. Lactose fermenters produces the black or dark center colonies (Positive result). Black colonies with green sheen are probably of E. coli.


Related Discussions:- Explain the most probable number (mpn) techniques

Describe steps of indications definitive surgical treatment, Describe steps...

Describe steps of Indications definitive Surgical Treatment? Indications for Definitive Surgical Treatment :  Surgery is indicated in all acute proximal dissections. Ind

Sql''s fifth concept - null, SQL's Fifth Concept - NULL Unfortunately...

SQL's Fifth Concept - NULL Unfortunately, SQL embraces a fifth concept, called NULL, an apparently simple little thing but one that has pervasive effects on our usual underst

Reason''s for mendel''s success, REASON'S FOR MENDEL'S SUCCESS 1.      ...

REASON'S FOR MENDEL'S SUCCESS 1.         Mendel selected only pure breeding varieties of pea ( Pisum sativum ) for his experiments. 2.         Mendel took only those traits

Name the drugs will help relieve for patient x, Patient X has blood plasma ...

Patient X has blood plasma levels of Parathyroid Hormone (PTH) that are always very high due to a tumor consisting of Parathyroid Gland cells that secrete high levels of PTH into t

Wind as a medium for dispersal, Wind as a medium for Dispersal Many mic...

Wind as a medium for Dispersal Many microorganisms, fungal spores and bacteria are freely transported over long distances even by moderate winds. In wind pollinated plants, the

Phloem, why phloem made of living cells

why phloem made of living cells

Describe how many bones are there in human foot, Describe how many bones ar...

Describe how many bones are there in human foot The human foot is a great mechanical marvel. It has 26 bones, 29 joints, and 42, various ligaments, a sensitive and protective s

Mendel''s second law not for two or more phenotypical traits, Why is not Me...

Why is not Mendel's second law always valid for two or more phenotypical traits of an individual? The Mendel's second law, or the law of the independent assortment, is valid fo

Explain taxonomists as synthesisers, Explain Taxonomists as Synthesisers? ...

Explain Taxonomists as Synthesisers? Your study of the earlier units of taxonomy, in particular its history must have made you aware that the field of taxonomy has changed cons

How is water importance to life, How is water's importance to life reflecte...

How is water's importance to life reflected in the normal range of internal pH values that living things are likely to have?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd