Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Immediate post-operative care to a cardiac patient, Immediate Post-operativ...

Immediate Post-operative Care The patient is accompanied to the ICU by a surgeon, anesthetist and the nurse who assisted for the surgery with portable ventilator and ECG

What are the implant selection errors, Implant Selection Errors There a...

Implant Selection Errors There are a plethora of commercially available implant systems and designs. Qualitative and quantitative nature of the available bone should be careful

What are some evolutionary advantages of animals, What are some evolutionar...

What are some evolutionary advantages of animals with complete digestive tube? The complete digestive tube permits animals to continuously feed themselves without waiting for r

Lumpy skin disease, L u mp y skin disease The disease, reported from...

L u mp y skin disease The disease, reported from Sudan in 1970 and Egypt in 1988, is caused by a member of the Capripox virus. It affects cattle and is restricted to African

Pulmonary valvotomy with infundibular resection, Pulmonary Valvotomy with I...

Pulmonary Valvotomy with Infundibular Resection :  Infundibular obstruction in cases of pulmonary valvar stenosis could be primary or secondary. 11' this obstruction is signi

Importance of counselling for diabetic patient, Q. Importance of counsellin...

Q. Importance of counselling for diabetic patient? Diabetes is a life-long illness. Life style modification and precautions taken by the patient can prevent complications and i

Sporophytic incompatibility, Sporophytic Incompatibility The recogniti...

Sporophytic Incompatibility The recognition and rejection reactions in a SSI system occur on the stigma surface, placing barriers for pollen germination or penetration of poll

Explain process of stress testing in women, Q. Explain process of Stress Te...

Q. Explain process of Stress Testing in Women? Estrogen has been implicated as a cause of ST depression. For years it seemed that estrogen protect women from coronary artery di

What is the normal duration of the menstrual cycle, What is the normal dura...

What is the normal duration of the menstrual cycle? How does the calendar contraceptive method work? The normal duration of the menstrual cycle is 28 days but it can vary betwe

What kind of structure is the crystalline lens, Q. What kind of structure i...

Q. What kind of structure is the crystalline lens? What is its function? The crystalline is a converging spherical lens this natural lens has the function to project images of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd