Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Respiration would cease even oxygen and food were supplied, If a tissue was...

If a tissue was heated to 65ºC for 10 minutes, respiration would cease even if oxygen and food were supplied. Why is this? A temperature of 65°C for 10 minutes would denature

Class of crustacea - malacostraca, Class of Crustacea - Malacostraca M...

Class of Crustacea - Malacostraca Malacostraca involves most of the larger forms such as crabs, lobsters, shrimps etc. and constitute the majority of crustacean species. The c

Explain the importance of surgery, Explain the Importance of Surgery? W...

Explain the Importance of Surgery? Well, surgery is that branch of medical science which has for its object the cure of local injuries or diseases, as wounds or fractures, trau

Adaptations to high wind velocity, Adaptations to high wind velocity Th...

Adaptations to high wind velocity The mechanical force of the wind and the grinding action of sand, dust, snow and other materials driven by it cause the plants to adapt themse

Presenting features of exstrophy of the bladder, Presenting Features ...

Presenting Features Anterior abdominal wall and anterior bladder wall is deficient and on the surface everted posterior wall of bladder with two ureteric orifices is seen.

Define feeding options for premature and lbw babies, Define Feeding Options...

Define Feeding Options for Premature and LBW Babies? Different workers have tried different method and since all methods are successful, it is dependent on the individual infan

Osmosis, everything about osmosis

everything about osmosis

Kingdom protista, how many phylum does kingdom protista has?

how many phylum does kingdom protista has?

Standard invert sugar solution, Q. Standard invert sugar solution? Rea...

Q. Standard invert sugar solution? Reagents Required 1) Standard invert sugar solution: Weigh accurately 0.985 g of sucrose and dissolve in 500 ml of water. Add 2 ml of

Tolerance model - models of succession, Tolerance Model - Models of Success...

Tolerance Model - Models of Succession In this model, the presence of early successional species is not essential, that is, any species can start succession. Some species are

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd