Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Stomata can open and close in response to changes in the co2, Stomata can o...

Stomata can open and close in response to changes in the CO 2 concentration inside the leaf. Would you expect stomata to open or close if the CO 2 concentration decreased? Explai

Explain technical factors influencing food production, Explain Technical fa...

Explain Technical factors influencing food production? Improvement in technology has a significant impact on productivity. Improvements may occur in seed production, fertilizer

Fluids requirement in chronic diarrhoea, Q. Fluids requirement in chronic d...

Q. Fluids requirement in chronic diarrhoea? Fluids: Intake should be liberal to minimize the risk of dehydration. Remember we read about fluid management in this section before

Change in structure of a protein, Q. Is it expected that a change in the pr...

Q. Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce furthermore functional consequences? Any change of the p

Define the concept of health care, Define the Concept of Health Care? W...

Define the Concept of Health Care? We are aware of the fact that health is a fundamental human right. Thus, it becomes imperative for the State to assume responsibility for the

Pre-embryonic development, Pre-Embryonic Development We had said in t...

Pre-Embryonic Development We had said in the starting of the unit that prenatal development can be divided into two phases, embryonic and foetal. The embryonic phase of devel

How to produce a similar sickling effect, Which of the following amino acid...

Which of the following amino acids would you expect to produce a similar sickling effect if placed at position 6? Select all that apply. 1. Arginine 2. Leucine 3. Lysine

What is the kind of nitrogen waste that mammals eliminate, Q What is the ki...

Q What is the kind of nitrogen waste that mammals eliminate? Like chondrichtian fishes and adult amphibians, mammals are ureotelic that is they excrete urea. Q. How do plac

Valves of the heart, Valves of the Heart There are four valves which ...

Valves of the Heart There are four valves which are flap-like structures that function to maintain unidirectional (forward) blood flow through the heart chambers. These va

What is thermoregulation in cold, What is Thermoregulation in Cold? Hea...

What is Thermoregulation in Cold? Heat production parallels the increase in O 2 uptake, the magnitude of which depends on the muscle mass .engaged in slivering or work and the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd