Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Coordination, how does impuses reaches the brain

how does impuses reaches the brain

What are cotyledons, What are cotyledons? Cotyledons, or seed leaves, a...

What are cotyledons? Cotyledons, or seed leaves, are structures formed by the embryo of angiosperms to absorb nutrients from the endosperm and to keep and transfer these nutrie

Define precaution for estimation of vitamin c in lemon juice, Define precau...

Define precaution for estimation of vitamin c in lemon juice? 1. Rinse all glassware with 3% metaphosphoric acid before you begin your practical and subsequently each time you

Define some texturization processes, Known physicochemical basis of some of...

Known physicochemical basis of some of these texturization processes are presented below: 1.Thermal Coagulation and Film Formation: Concentrated soy proteins can be thermally

Explain arterial switch operation surgery, Explain Arterial Switch Operatio...

Explain Arterial Switch Operation Surgery? This is the operation of choice for simple transposition of the great arteries as it ensures anatomical correction. The approach is t

Poultry and duck diseases-avian influenza, Avian influenza The etiologi...

Avian influenza The etiological a gentisa via ninfluenz a type A virus und e r the family Orthomyxoviridae. Avian influenza pandemics in Pakistan, Nepal and Myanamar (1995) and

Water cycle-hydrological cycle, Water Cycle (Hydrological Cycle) The mo...

Water Cycle (Hydrological Cycle) The movement of water on the earth is continuous and forms many complex inter-related loops (Figure shown below). Cycling of water involves atm

Sectioned and formed meats, S e c t i o ned and Formed Meats Thes...

S e c t i o ned and Formed Meats These are technically speaking restructured products, since they are partially disassembled and then reassembled to form products resembl

Define carbohydrates needs in postoperative nutritional care, Define Carboh...

Define Carbohydrates needs in Postoperative Nutritional Care? Carbohydrates ensure the use of protein for tissue synthesis and energy required for increased metabolic demands.

Starch can be used by the germinating seed, Starch is one of the most commo...

Starch is one of the most common storage products in seeds. What happens to the starch before it can be used by the germinating seed? Starch is insoluble and has to be changed,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd