Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Define fish as a rich source of protein, Define Fish as a rich source of pr...

Define Fish as a rich source of protein? The edible portion is skeletal muscles of the body. Even though the skeletal muscles of different animals are basically similar, fish s

Define cercaria larva, Define Cercaria Larva? Cercaria  larva is the f...

Define Cercaria Larva? Cercaria  larva is the fourth  larval stage  in  the life-cycle of F, hepatica. It is a free living stage produced  by  the  redia  larva. 1. It has

Explain functions of ascorbic acid, Explain Functions of Ascorbic Acid? ...

Explain Functions of Ascorbic Acid? Vitamin C is easily oxidized, and the majority of its functions in vivo rely on this property. It plays a key role in the body's synthesis o

Define seminal plasma in human male, Seminal plasma in human males is rich ...

Seminal plasma in human males is rich in : 1. fructose and calcium 2. glucose and calcium 3. DNA and testosterone 4. ribose and potassium Fructose and Calcium

Diagrammatic representation of implant placement, Diagrammatic representati...

Diagrammatic representation of Implant placement Implant placementIt is located directly apical to the tooth it is replacing and not in an embrasure space. The angulati

Describe the regulation of pyruvate dehydrogenase, Describe the regulation ...

Describe the regulation of pyruvate dehydrogenase through covalent modification. PDH exists in 2 forms :  Inactive, phosphorylated and Active, dephosphorylated. The active form

Psychology, Which theory focuses on how people are different than animals? ...

Which theory focuses on how people are different than animals? Functionalism Eclecticism Humanism Behaviorism

Osteogenesis - production of bone on soft tissue, Osteogenesis: Production ...

Osteogenesis: Production of Bone on Soft Tissue Now that we know the players in bone adaptation, we will look at the ways in which bone may be created and modified. There are t

Induction of disease resistance in plants by heat-shock, Induction of disea...

Induction of disease resistance in plants by heat-shock The fungus Cladosporium cucumerinum infects cucumber and causes disease in the plant. However, if seedling of cucumber

How are antivenoms produced, How are antivenoms produced? Why are antivenom...

How are antivenoms produced? Why are antivenoms an example of passive immunization? Antivenoms are getting by the following process: the venom (antigen) is inoculated into othe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd