Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

What is the characteristic of cardiac muscle, What are the characteristics ...

What are the characteristics of cardiac muscle that allow it to be highly resistant to fatifue?

Is there a exchange of cells between the mother, Is there a exchange of cel...

Is there a exchange of cells between the mother and the fetus through the placenta? Under normal conditions, there is not a passage of cells across the placenta during gestati

Vertebrate, Basic characters of vertebrate classes

Basic characters of vertebrate classes

Actions of estrogens, Actions of Estrogens The most general effect ...

Actions of Estrogens The most general effect of the estrogens is to promote tissue growth. It is more pronounced in the accessory sex tissues. Estrogens stimulate cell divi

Consumptive use values, This means the non-market value of natural products...

This means the non-market value of natural products such as firewood, game and fodder that do not pass through a market or product preparation. Indigenous people in developing cou

Enhance the expression of the sefp testing, Baculovirus is widely used for ...

Baculovirus is widely used for expressing recombinant proteins. Structural of baculovirus is a rod-shaped nucleocapsid containing genomic DNA. The baculovirus Autographa California

Mode of hormone action through extracellular receptors, MOD E OF HORMONE A...

MOD E OF HORMONE ACTION THROUGH EXTRACELLULAR RECEPTORS - The molecules of amino acid derivatives, peptides or polypeptide (protein) hormones bind to specific receptor molecul

Determine about the scales developed by golden, Determine about the scales ...

Determine about the scales developed by golden Several other scales have been developed by Golden and various collaborators, all of which are based on different ways of scoring

Explain the parental gametes and recombinant gametes, In genetic recombinat...

In genetic recombination by crossing over what is the difference between parental gametes and recombinant gametes? Parental gametes are those gametes that keep the original lin

Explain cap site, Cap site Two usages are given below: 1] In eukaryotes...

Cap site Two usages are given below: 1] In eukaryotes, the cap site is position in the gene at which transcription begins, and really should be called the "transcription initia

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd