Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Hyperlipidemia, Dyslipidemia is an important correctable factor for Coronar...

Dyslipidemia is an important correctable factor for Coronary Artery Disease. There is a strong, independent, continuous, and graded relation between total cholesterol (TC) or low-

Contagious bovine pleuropneumonia, C o n t a g i o u s bovine pleu...

C o n t a g i o u s bovine pleuropneumonia It is a disease of bovines characterized by high rise of body temperature and difficulty in respiration. E t iolo

Define the indications for root-end resection apicoectomy, Define the Indic...

Define the Indications for Root-End Resection Apicoectomy a) Persistent..... "Exactly the SAME of Curettage except the biopsy. b) When the apical portion of the root canal s

Benthic zone - organisation of the marine ecosystem, Benthic Zone - Organis...

Benthic Zone - Organisation of the Marine Ecosystem The benthic zone is divisible into sub zones horizontally. These are depicted in a cross section portion of the marine habi

Describe the technique of food processing, Technique of food processing ...

Technique of food processing Freezing is another ancient technique of food processing, which allows the transportation of perishable food items for long distances from producti

Skin., what is the difference between fair skin and dark skin?

what is the difference between fair skin and dark skin?

Deoxyribonucleic acid (dna), Deoxyribonucleic acid (DNA) is the nucleic ac...

Deoxyribonucleic acid (DNA) is the nucleic acid composed of two polynucleotide strands wound around the central axis to form a double helix; the repository of genetic information.

Eye lens, LEN S -  In lumen of eye ball, a large colourless, transp...

LEN S -  In lumen of eye ball, a large colourless, transparent, more convex on back side, lens present covered by lens capsule. When white tissues are formed in it, it b

Explain briefly nucleic acids dna and rna, Explain briefly Nucleic Acids (D...

Explain briefly Nucleic Acids (DNA & RNA)? Nucleic Acids (DNA & RNA) :  Nucleic acids are the molecules that contain genetic information that is inherited, and are responsibl

Define how to prevent the cancer, Define How to Prevent the Cancer? A c...

Define How to Prevent the Cancer? A continuing and urgent need is there to take all steps to prevent cancer. Cancer rates are set to increase another 50 percent by the year 202

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd