Explain the decolourizing agent - ziehl-neelsen method, Biology

Assignment Help:

Explain the Decolourizing Agent - Ziehl-Neelsen Method?

Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allowed to cool to make waxy substances hardened. As primary stain is more soluble in cellular waxes than in decolourizing solution, acid-fast bacteria retain the primary stain. However, all non-acid fast bacteria loose primary stain because of the lack of cellular waxes.


Related Discussions:- Explain the decolourizing agent - ziehl-neelsen method

Define the advatages of potassium, Define the advatages of Potassium Th...

Define the advatages of Potassium The gross impact of these effects on crop production is manifested  in several ways. Perhaps the first visible indication of a potassium defic

Plant reproduction, How does being able to reproduce asexually benefit the ...

How does being able to reproduce asexually benefit the plant?

Bioenergetics, Energy Cycle  of Living Being Source of energy :All  mat...

Energy Cycle  of Living Being Source of energy :All  matter  locks  energy in the  from of   bonds  between component  molecules and   atoms. This  energy is the chemical  ener

How much sds need for 50 ml, How much SDS do you need for 50 ml of a 20% SD...

How much SDS do you need for 50 ml of a 20% SDS solution in water(weight/ volume)? SDS is a solid.

Transport in phloem, Transport in Phloem The basic necessities of plan...

Transport in Phloem The basic necessities of plants, water, are taken up by the roots. Another purpose served by the roots is to absorb water soluble mineral nutrients from th

Name two features of eukaryotic cells, How are the organelles of a single c...

How are the organelles of a single cell like the organs of a multicellular organism? Name two features of eukaryotic cells that prokaryotic cells lack.

Tracheostomy care, Tracheostomy Care A tracheostomy is an external ope...

Tracheostomy Care A tracheostomy is an external opening made into the trachea in order to provide an artificial airway (an opening is made in the 2nd and 3rd or 4th trach

Determine the process of rna editing, Which of the following is a true stat...

Which of the following is a true statement regarding the process of RNA editing? A. RNA editing alters the sequence of the mRNA message thereby permitting for an enhance in pro

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd