Explain the composition of macconkey agar, Biology

Assignment Help:

Explain the Composition of MacConkey Agar?

Bactopeptone - 17.0 gm

Protease Peptone - 3.0 gm

Lactose - 10.0 gm

Bile salt mixture - 1.5 gm

Sodium chloride - 5.0 gm

Agar - 13.5 gm

Neutral Red - 0.03 gm

Crystal Violet - 0.001 gm

Distilled water - 1000 ml

pH - 7.1

MacConkey Agar is a specialized media used for selection and differentiation among different bacterial species. It contains crystal violet, which inhibits the growth of gram positive but permits gram negative to grow (selection).  Presence of lactose, bile salts and neutral red in medium allows differentiation of bacteria on basis of their ability to ferment lactose. This medium is used to differentiate members of Enterobacteriaceae, which are morphologically and biochemically related into 2 groups.


Related Discussions:- Explain the composition of macconkey agar

Pulmonary autograft and ross procedure, Pulmonary Autograft and Ross Proced...

Pulmonary Autograft and Ross Procedure :  Sir Donald Ross in 1967 introduced the concept of using patient's own pulmonary valve (autograft) for aortic valve replacement and a pul

What is difference in electrical charge between two points, The difference ...

The difference in electrical charge between two points: Select one: is called the potential difference between those points. is called the diffusion potential between those p

What does myeloid immunodeficiency cause, Myeloid immunodeficiency causes p...

Myeloid immunodeficiency causes phagocytic function, which is impaired. Those who are affected with this will undergo with enhance in susceptibility to bacterial infection.

Explain water-soluble and fat-soluble vitamins, What is the difference betw...

What is the difference between water-soluble and fat-soluble vitamins? Why can fat-soluble vitamins cause harm when ingested in excess? Water-soluble vitamins are those soluble

Why are glucorticoids used in transplant patients, Why are glucorticoids us...

Why are glucorticoids used in transplant patients? Patients with transplanted organs are prone to host versus graft rejection as their own immune system tends to attack the gr

Plasmid, Plasmid  is the class of the circular, extrachromosomal, autonomou...

Plasmid  is the class of the circular, extrachromosomal, autonomously replicating, DNA elements found in number of bacteria. Contain origins of the replication to ensure their main

Standard titration - estimation of vitamin c in lemon juice, Explain Standa...

Explain Standard titration - Estimation of Vitamin C in Lemon Juice? Pipette 5 ml of standard ascorbic acid solution into a 100 ml conical flask. Fill the burette with the dye s

Illustrate the nature of the stimulus, Q. What is the nature of the stimulu...

Q. What is the nature of the stimulus received and transmitted by the neurons? Neurons transmit and receive chemical stimuli through neurotransmitters released in the synapses.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd