Explain the Aseptic Technique - Sub Culturing?

Aseptic technique is a series of procedures used to prevent contamination of cultures and sterile culture media. It is one of the first methods to be learned by the students of microbiology. As microorganisms are ubiquitous, unwanted microorganisms or contaminants can enter into pure cultures from air, working bench, hands or surroundings. Therefore, it is essential to adopt microbiological techniques that are designed to avoid contaminants. We shall learn about these techniques now in this practical and then practice these techniques.