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Restriction endonucleases are enzymes which:
1. make cuts at specific positions within the DNA molecule
2. recognize a specific nucleotide sequence for binding of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule
Make cuts at specific positions within the DNA molecule
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Cosmid: The type of artificially constructed vector taken in use for cloning the 35-45 kb of DNA. These are plasmids carrying the phage l cos site (which permits packaging into l
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excretory organs of protozoa
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