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Explain Methods for Coliform Detection in Water?
Sanitary condition of drinking water can be determined by counting the coliform bacteria. It is a reliable and reproducible indicator of faecal contamination in all public water supplies. Both qualitative and quantitative methods can be used. Commonly used methods for coliform detection in water samples are:
i) Most probable number procedure, and
ii) Membrane filtration procedure
Other tests like presence-absence test, coli test, defined substrate test and molecular techniques can be used to detect coliforms in water and other environment. In a well regulated water supply system, coliform tests should be negative. Positive tests indicates a breakdown in purification or distribution system.
Blotting is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove
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